Effect of ammonium supplementation on production of poly-B-hydroxybutyric acid byAlcaligenes eutrophus in batch culture

Bitar, Ahed; Underhill, S E

doi:10.1007/bf01030753

Cited by 23 publications

(13 citation statements)

References 8 publications

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“…Similar effect was observed by Bitar and Underhill (1990) in their batch culture of Alcaligenes eutrophus. They noted that without the ammonium supplementation at the beginning of PHASCL accumulation phase, the PHASCL accumulation rate (g PHA L -1 h -1 ) was below 0.1 g L -1 h -1 .…”

Section: Kinetics Of Phamcl Biosynthesis In Batch Fermentationsupporting

confidence: 79%

Production of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed-Batch Fermentations

Annuar¹,

Tan²,

Ibrahim³

et al. 2006

MJM

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The kinetics of medium-chain-length poly(3-hydroxyalkanoates), PHAMCL production by Pseudomonas putida PGA1 in batch and fed-batch fermentations were studied. With saponified palm kernel oil (SPKO) supplying the free fatty acids mixture as the sole carbon and energy source, PHAMCL accumulation is encouraged under ammonium-limited condition, which is a nitrogen stress environment. The amount of PHAMCL accumulated and its specific production rate, qPHA were influenced by the residual ammonium concentration level in the culture medium. It was observed that in both fermentation modes, when the residual ammonium was exhausted (< 0.05 gL -1 ), the PHAMCL accumulation (11.9%) and qPHA (0.0062 h -1 ) were significantly reduced. However, this effect can be reversed by feeding low amount of ammonium to the culture, resulting in significantly improved PHAMCL yield (71.4%) and specific productivity (0.6 h -1 ). It is concluded that the feeding of low ammonium concentration to the culture medium during the PHAMCL accumulation has a positive effect on sustaining the PHAMCL biosynthetic capability of the organism. It was also found that increasing SPKO concentration in the medium significantly reduced (up to 50%) the volumetric oxygen transfer coefficient (KLa) of the fermentation system.

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Section: Kinetics Of Phamcl Biosynthesis In Batch Fermentationsupporting

confidence: 79%

Production of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed-Batch Fermentations

Annuar¹,

Tan²,

Ibrahim³

et al. 2006

MJM

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“…Production of PHA is most frequently studied under nitrogen starvation despite the fact that addition of a nitrogen source during the production phase has been shown to increase both the productivity and the substrate conversion yield for PHB (Suzuki et al 1986a(Suzuki et al , 1986bBitar andUnderhill 1990: Lee et al 1993). Copolymer production by A/ca/~Tenes SH-69 using glucose as the sole carbon source, has been shown by Rhee et al pcrmanenl addrcss: Dept of Chcmical Enginccring -UFSC-Florian6polis-S. C.…”

Section: Introductionmentioning

confidence: 99%

Maintaining a controlled residual growth capacity increases the production of polyhydroxyalkanoate copolymers by Alcaligenes eutrophus

et al. 1996

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The manner in which copolymer poly(HB-co-HV) production was influenced by different methods of limiting cell proliferation during the production phase was examined. Polymer production was significantly improved in fermentation strategies in which some growth was maintained, either by linear or exponential nitrogen source feeding as compared to cultures in which nitrogen supply was totally interrupted. Improved volumetric productivities were obtained in cultures fed with NH4OH and the proportion of 3HV incorporated was approximately twofold higher in these cultures. These performance improvements were due to higher specific rates of glucose and propionate consumption in cultures in which true growth capacity was maintained

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“…The presence of ammonium as a source of nitrogen is an important requirement during the growth phase in order to maximize the concentration of biomass responsible for accumulation of PHB. This nitrogen source has been studied by several researchers vis-à-vis its impact on maximizing the production of PHB by A. eutrophus (3,15,21,23). The ammonium salts mainly used for the growth of A. eutrophus were (NH 4 ) 2 SO 4 (14,15,20) and NH 4 Cl (3,21).…”

mentioning

confidence: 99%

“…This nitrogen source has been studied by several researchers vis-à-vis its impact on maximizing the production of PHB by A. eutrophus (3,15,21,23). The ammonium salts mainly used for the growth of A. eutrophus were (NH 4 ) 2 SO 4 (14,15,20) and NH 4 Cl (3,21). Bitar and Underhill (3) studied the effect of ammonium chloride supplementation in the presence of glucose on the production of poly-␤-hydroxybutyric acid by A. eutrophus; the maximum specific growth rate was obtained with 0.5 g of NH 4 Cl per liter, and low levels of NH 4 ϩ supplied to the culture during the PHB accumulation phase resulted in higher PHB production.…”

mentioning

confidence: 99%

Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate

Beaulieu¹,

Beaulieu²,

Melinard³

et al. 1995

Appl Environ Microbiol

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The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammonium sources such as sulfate, nitrate, phosphate, and chloride salts and those of different ammonium sulfate concentrations were evaluated. The best growth and PHB production were obtained with ammonium sulfate; however, NH 4 ؉ concentrations between 0.5 and 1.5 g/liter showed no significant difference. Ammonium sulfate was therefore used as the sole source of NH 4 ؉ for experiments with cane molasses as the growth activator. Optimal growth and PHB production were obtained with 0.3% molasses. However, the yields of biomass (39 to 48%) and PHB (17 to 26%) varied significantly among the different ammonium substrates and cane molasses concentrations.

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Effect of ammonium supplementation on production of poly-B-hydroxybutyric acid byAlcaligenes eutrophus in batch culture

Cited by 23 publications

References 8 publications

Production of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed-Batch Fermentations

Production of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed-Batch Fermentations

Maintaining a controlled residual growth capacity increases the production of polyhydroxyalkanoate copolymers by Alcaligenes eutrophus

Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate

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