Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate

Beaulieu, Martin; Beaulieu, Y.; Melinard, J; Pandian, S; Goulet, J.

doi:10.1128/aem.61.1.165-169.1995

Cited by 76 publications

(32 citation statements)

References 20 publications

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“…During the fermentation, a pH drop resulted due to the production of H 2 SO 4 . Similar observations have been reported for different bacteria (Repaske and Repaske 1976;Beaulieu et al 1995). The ef®ciency of PHB synthesis obviously depends on the type of nitrogen source used (Bormann et al 1998a).…”

Section: Discussionsupporting

confidence: 81%

The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017

2002

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Aims: To optimize the nutritional and environmental conditions for growth of and poly±b-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. Methods and Results: An isolate, identi®ed as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammonium sulphate were found to be the most suitable for growth and PHB accumulation. The overall maximum value of PHB (%) in cells, PHB yield (Y p/s ) and productivities (Q p and q p ) were 69á4 0á4% dry cell weight (DCW), 0á21 g p g S ±1 , 0á104 0á012 g p l ±1 h ±1 and 0á03 g p g x ±1 h ±1, respectively when grown in a medium containing 20 g s l ±1 sucrose, supplemented with di-ammonium sulphate. The addition of beef extract increased the value of PHB (%) in cells, PHB yield and productivities by 17á58 0á3, 23á8, 19á23 0á3 and 13á8 0á2%, respectively. The overall maximum values of PHB (% DCW), PHB yield and productivities were obtained at pH 7á0 0á1, temperature 30 0á5°C, agitation 650 rev min ±1 and oxygen transfer rate 3á8 mmol O 2 l ±1 h ±1 . Conclusions: Sucrose, glucose and fructose were found to be more suitable for cell growth and PHB accumulation, but sucrose was less expensive than glucose. Among the nitrogen sources, beef extract and di-ammonium sulphate promoted PHB synthesis. The accumulation of PHB was observed to be growth associated. Signi®cance and Impact of the Study: Gram-positive bacteria have not been reported to accumulate large amounts of polyhydroxyalkanoate and hence have not been considered as potent candidates for industrial production. A number of Bacillus spp. have been reported to accumulate 9±44á5% DCW PHB. By comparison, Bacillus RLJ B-017 contained 69á4 0á4% DCW PHB. Therefore, this strain has been considered as a potent organism for industrial interest. A relatively high yield of PHB was obtained in this wild strain and PHB synthesis was independent of nutrient limitation. The conditions for the higher PHB yield and productivity will be optimized in the next phase using fed-batch culture.

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Section: Discussionsupporting

confidence: 81%

The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017

2002

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“…Because molasses is a runoff syrup of a byproduct from the final stage of crystallization in the sugar production process, it is one of the most economical carbon sources in microbial industry. Therefore, molasses has been widely used as a substrate in fermentations such as in the production of lactic acid (12), polyhydroxybutyrate (13), ethanol (14), pullulan (15), and xanthan gum (16). However, no BC production using molasses as a carbon source has been reported.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose Production by Fed-Batch Fermentation in Molasses Medium

2004

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Batch and fed-batch fermentations for bacterial cellulose (BC) production using molasses as a carbon source by Acetobacter xylinum BPR2001 were carried out in a jar fermentor. For improvement of BC production, molasses was subjected to H2SO4-heat treatment. The maximum BC concentration by this treated molasses increased 76%, and the specific growth rate increased 2-fold compared with that by untreated molasses. In batch fermentation, when the initial sugar concentrations of H2SO4-heat-treated molasses were varied from 20 to 70 g/L, the highest value of maximum BC concentration of 5.3 g/L was observed at 20 g/L. BC production in intermittent fed-batch (IFB) fermentation was conducted referring to the data in batch fermentation, and the highest BC production of 7.82 g/L was obtained when 0.2 L of molasses medium was added five times. When continuous fed-batch (CFB) fermentations were conducted, maximum BC concentration was obtained with a feeding rate of 6.3 g-sugar/h, which was derived from the optimal IFB experiment.

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“…However, it is toxic to microbial cells causing decreased growth . PHB production was high when using ammonium sulfate as nitrogen source due to the uptake of ammonium at a high rate in the presence of sulfate anion .…”

Section: Discussionmentioning

confidence: 99%

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

2018

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Polyhydroxyalkanoates (PHAs) has been paid great attention because of its useful thermoplastic properties and complete degradation in various natural environments. But, at industrial level, the successful commercialization of PHAs is limited by the high production cost due to the expensive carbon source and recovery processes. Pseudomonas mendocina PSU cultured for 72 h in mineral salts medium (MSM) containing 2% (v/v) biodiesel liquid waste (BLW) produced 79.7 wt% poly(3-hydroxybutyrate) (PHB) at 72 h. In addition, this strain produced 43.6 wt% poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with 8.6 HV mol% at 60 h when added with 0.3% sodium propionate. The synthesized intracellular PHA granules were recovered and purified by the recently reported biological method using mealworms. The weight average molecular weight (M ) and number average molecular weight (M ) of the biologically extracted PHA were higher than that from the chloroform extraction with comparable melting temperature (T ) and high purity. This study has successfully established a low-cost process to synthesize PHAs from BLW and subsequently confirmed the ability of mealworms to extract PHAs from various kinds of bacterial cells.

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Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate

Cited by 76 publications

References 20 publications

The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017

The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017

Bacterial Cellulose Production by Fed-Batch Fermentation in Molasses Medium

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

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