Effect of amine groups in the synthesis of Ag nanoparticles using aminosilanes

Kumar

2017

Asian J Pharm Clin Res

Objective: This study demonstrates a simple, cost-effective protocol for biosynthesis of stable silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) from Hibiscus rosa-sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: AgNPs and AuNPs were biosynthesized from H. rosa-sinesis. The antibacterial activities of biosynthesized AgNPs (bio-AgNPs) and biosynthesized AuNPs (bio-AuNPs) were evaluated against 9 pathogenic bacterial species Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Biological synthesized AgNP/AuNPs were characterized by an ultraviolet-visible (UV-Vis) spectrophotometer, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The existence of silver and gold ions in the extracts was conformed (primary confirmation) through UV-Vis and the maximum plasmon peak was observed at 402 and 530 nm of bio-AgNPs and bio-AuNPs, respectively. Bio-AgNPs were obtained in 13.01-28.14 nm size range, whereas bio-AuNPs were in 6.32-18.19 nm size range after analysis of TEM images. The results of FTIR spectra indicate that the bio-AuNPs were bound to amine groups and bio-AgNPs to carboxylate ion groups. The antibacterial activities of AgNP/AuNP, the zone of inhibition significantly increased with the increases of concentrations of bio-AgNPs in all pathogenic bacterial species except in the case of S. epidermidis at 50%, Streptococcus aerogenes and A. hydrophila at 70%, whereas in the case of bio-AuNPs antibacterial activities were displayed only against B. subtilis at 20% and 100% concentration. Conclusion:This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from H. rosa-sinensis leaf extract and insights to their potential applicability as an alternative antibacterial agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria.

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Metal Nanoparticles Synthesized From Hibiscus Rosa Sinesis and Their Antibacterial Activity Estimation Against Nine Pathogenic Bacteria

Kumar

2017

Asian J Pharm Clin Res

Int. J. Environ. Sci. Technol.

“…In addition, relatively recently, a new form of Ag metal has been engineered comprising of Ag nanoparticles (Ag NPs) that can have novel and size-related physicochemical properties differing significantly from those from larger particles (Fabrega et al 2011). Ag NPs are widely used in medicine, cosmetics, environmental remediation or electronic devices (Fabrega et al 2011) and have distinctive physicochemical properties, including surfaceenhanced Raman scattering, high electrical and thermal conductivity, chemical stability, catalytic activity, and nonlinear optical behavior (Capek 2004;Frattini et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Histopathological effects of waterborne silver nanoparticles and silver salt on the gills and liver of goldfish Carassius auratus

Abarghoei

Hedayati

Ghorbani

et al. 2016

This study aimed to compare histopathological effect of waterborne silver nanoparticles and silver salt (AgNO 3 ) on the gills and liver of Carassius auratus. Therefore, one hundred and five live specimens of goldfish were obtained and treated in five aquariums with 0, 0.01, 0.025, 0.05, and 0.1 ppm of AgNO 3 and 0, 0.1, 0.5, 1, and 5 ppm of Ag nanoparticles (mean particle size of 5 nm). Fish were sampled after 14 days of exposure. Results showed that the kinds of pathologies observed with Ag NPs were broadly of the same type as AgNO 3 including hyperplasia, edema and lifting of the gill epithelium, and lamellar fusion of the gills, and hemosiderosis, hemorrhage, hydropic swelling, and pyknotic nuclei of the liver. Overall, the data showed that although Ag nanoparticles and AgNO 3 pathology were similar, but Ag nanoparticles caused less injury than AgNO 3 in the gills and liver of goldfish. Therefore, it is more proper to use nanoform of Ag in industrials.

“…The exceptional broad-spectrum bactericidal activity of silver and the relatively low cost of manufacturing AgNP have made them extremely popular in medicine and a broad range of consumer materials, including plastics, soaps, pastes, metals and textiles [2][3][4]. Silver nanoparticles exhibit a wide range of unique properties, i.e.…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticle accumulation by aquatic organisms – neutron activation as a tool for the environmental fate of nanoparticles tracing

et al. 2014

Abstract. Water environments are noted as being some of the most exposed to the infl uence of toxic nanoparticles (NPs). Therefore, there is a growing need for the investigation of the accumulation and toxicity of NPs to aquatic organisms. In our studies neutron activation followed by gamma spectrometry and liquid scintillation counting were used for studying the accumulation of silver nanoparticles (AgNPs) by freshwater larvae of Chironomus and fi sh Danio rerio. The infl uence of exposition time, concentration and the source of nanoparticles on the effi ciency of AgNP accumulation were studied. It was found that AgNPs are effi ciently accumulated by Chironomid larvae for the fi rst 30 hours of exposition; then, the amount of silver nanoparticles decreases. The silver content in larvae increases together with the NP concentration in water. Larvae which have accumulated AgNPs can be a source of nanoparticles for fi sh and certainly higher levels of Ag in the trophic chain. In comparison with water contamination, silver nanoparticles are more effi ciently accumulated if fi sh are fed with AgNP-contaminated food. Finally, it was concluded that the applied study strategy, including neutron activation of nanoparticles, is very useful technique for tracing the uptake and accumulation of NPs in organisms.