Histopathological effects of waterborne silver nanoparticles and silver salt on the gills and liver of goldfish Carassius auratus

Abstract

This study aimed to compare histopathological effect of waterborne silver nanoparticles and silver salt (AgNO 3 ) on the gills and liver of Carassius auratus. Therefore, one hundred and five live specimens of goldfish were obtained and treated in five aquariums with 0, 0.01, 0.025, 0.05, and 0.1 ppm of AgNO 3 and 0, 0.1, 0.5, 1, and 5 ppm of Ag nanoparticles (mean particle size of 5 nm). Fish were sampled after 14 days of exposure. Results showed that the kinds of pathologies observed with Ag NPs were broadly of the same type as AgNO 3 including hyperplasia, edema and lifting of the gill epithelium, and lamellar fusion of the gills, and hemosiderosis, hemorrhage, hydropic swelling, and pyknotic nuclei of the liver. Overall, the data showed that although Ag nanoparticles and AgNO 3 pathology were similar, but Ag nanoparticles caused less injury than AgNO 3 in the gills and liver of goldfish. Therefore, it is more proper to use nanoform of Ag in industrials.

Histopathological impairment of common carp (Cyprinus carpio) induced through povidone‐iodine exposure

“…Beneficial role of histopathological biomarker in monitoring of pollutants in aquatic ecosystem has been confirmed in previous studies (Abarghuei et al, ; Banaee, Sureda, Mirvagefei, & Ahmadi, ; Camargo & Martinez, ). Due to entrance of disinfectants materials to aquatic ecosystems, betadine penetrates to water and it had been shown that is toxic for carp, so this study is done to determine the possible effects of betadine solution on the liver and gill tissue of common carp.…”

“…For this study120‐carp (17 ± 3 g) were kept for 14 days in the 100‐l aquarium. For adaptation to experimental conditions, fish in photoperiod of 13 hr of darkness and 11 hr of light were kept and maintained twice a day at a rate of 2% of body weight with a functional commercial diet (Abarghuei et al, ). Water replaced every 24 hr during the experimental period, Water physicochemical parameters measurements include (21 ± 1°C: water temperature, pH 6.7–7.9, dissolved oxygen concentration: 7–9 mg/L and water hardness: 210 mg calcium carbonate per liter).…”

“…Histological and pathological lesions have been widely applied as biomarkers in monitoring of aquatic condition exposed to pollutants (Hedayati & Hassan Nataj Niazie, ). Histopathological biomarkers in environmental monitoring includes examining specific purpose organs, such as kidney, liver, and gills, that are responsible for biotic functions, such as repulse, breathing and the cumulation and biotransformation of xenobiotics in the fish (Abarghuei, Hedayati, Ghorbani, Kolangi, & Bagheri, ). Fish gills are responsible for gas exchange, osmotic adjustment, acid–base regulation, disposal of waste nitrogen, the endocrine system and have a thin epithelial layer and wide range of setting, so could be acclaimed as an important organ which are exposed to direct toxins and pollutants, therefore structural changes examination of the gill is a suitable indicator of toxins or pollutants stress conditions (Vajargah, Mohamadi Yalsuyi, Hedayati, & Faggio, ).…”

Effect of long‐term exposure of silver nanoparticles on growth indices, hematological and biochemical parameters and gonad histology of male goldfish (Carassius auratus gibelio)

“…Toxicity of silver to different fish in vivo has been investigated by researchers (Abarghoei, Hedayati, Ghorbani, Miandareh, & Bagheri, ; Janes & Playle, ; Wood, Playle, & Hogstrand, ; Yue et al, ; Zhou, Nichols, Playle, & Wood, ). In aquatic environment, Ag‐NPs release silver ions, which is can cause toxicity to fish by blocking the Na + K + ATPase and disturbing the ion regulation of Na + and Cl − ions across the gills (Bury & Wood, ).…”

I SEMAVE - Semana Acadêmica de Medicina Veterinária da UNIR

“…Além disso, o parênquima hepático apresentava alto número de células de Kupffer, degeneração hialina, dilatação do espaço sinusoidal, vacuolização e retração de hepatócitos, estas alterações também foram descritas no peixe Acipenser baerii expostos à nanopartículas de prata (OSTASZEWSKA et al, 2016). Nas brânquias foi observado necrose lamelar com perda do epitélio lamelar por descolamento (Figura A3), edema e infiltrado polimorfonuclear, alterações também observadas por Abarghoei et al (2016) em Carassius auratus expostos a AgNP. No rim foi observado, perca do espaço de Bowman, exsudato inflamatório, necrose glomerular, necrose tubular (Figura A4), congestão e infiltrado polimorfonuclear e melanomacrófagos.…”