Effect of Amelotin on Bone Growth in the Murine Calvarial Defect Model

Tanaka, Daiki; Ikeda, Yuichi; Ikeda, Eri; Yokose, Mako; Ganß, Bernhard; Iwata, Takanori

doi:10.1007/s10439-021-02867-z

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…Amelotin (AMTN) is specifically expressed by maturation stage ameloblasts (Iwasaki et al, 2005), is detectable in the ameloblast‐derived junctional epithelium (JE) (Moffatt et al, 2006; Somogyi‐Ganss et al, 2012) that seals the periodontal ligament to the root, and is expressed de novo at the gingiva/tooth interface during later stages of wound healing (Nishio et al, 2010). As the adherence of collagen membranes to dentine is enhanced by rh‐AMTN and induces bone repair by promoting hydroxyapatite mineralization in vivo (Tanaka et al, 2021), we examined the effect of AMTN on reattachment. Finally, as an alternative to suturing mucoperiosteal flaps, we examined whether calcium phosphate cement promotes attachment of the collagen membranes to the root surface.…”

Section: Introductionmentioning

confidence: 99%

Screening of functionalized collagen membranes with a porcine periodontal regeneration model

et al. 2022

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ObjectivesCurrent methods for periodontal regeneration do not promote collagen fiber insertions into new bone and cementum. We used a pig wound model to screen different functionalized collagen membranes in promoting periodontal reattachment to root surfaces.MethodsTreatment groups included (1) control with no membranes, (2) collagen‐coated membranes, (3) membranes with insulin‐like growth factor‐1 (IGF‐1), (4) membranes with amelotin, or (5) membranes attached with calcium phosphate cement (CPC), or with CPC combined with IGF‐1. Flap procedures were performed on mandibular and maxillary premolars of each pig.ResultsHistomorphometric, micro‐CT, and clinical measurements obtained at 4 and 12 weeks after surgery showed cementum formation on denuded roots and reformation of alveolar bone, indicating that the pig model can model healing responses in periodontal regeneration. Calcium phosphate cement simplified procedures by eliminating the need for sutures and improved regeneration of alveolar bone (p < 0.05) compared with other treatments. There was a reduction (p < 0.05) of PD only for the IGF group. Large observed variances between treatment groups indicated that a priori power analyses should be conducted to optimize statistical analysis.ConclusionsPigs can model discrete elements of periodontal healing using collagen‐based, functionalized membranes. Screening indicates that membrane anchorage with calcium phosphate cements improve regeneration of alveolar bone.

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