Effect of ambient temperature variations on an indigenous microalgae-nitrifying bacteria culture dominated by Chlorella

González-Camejo, J.; Aparicio, S.; Ruano, M.V.; Borrás, L.; Barat, R.; Ferrer, J.

doi:10.1016/j.biortech.2019.121788

Cited by 54 publications

(37 citation statements)

References 48 publications

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“…AOB competition for ammonium uptake had significant effect on MPBR performance, as suggested byGonzález-Camejo et al (2019c). For this reason, another PLS analysis was carried out to consider NOxR as a response (see section 3.4).Many other variables appeared to be correlated with MPBR performance, which agrees withCho et al (2019) and corroborates the difficulty of controlling outdoor microalgae cultivation.…”

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confidence: 67%

“…The competition between microalgae-nitrifying bacteria for ammonium uptake has been identified as a highly relevant factor in the performance of a mixed microalgae culture (Galès et al, 2019;González-Camejo et al, 2019c), so that determining the most important parameters in nitrifying bacteria activity would help to maintain bacteria growth at a minimum and favour microalgae growth.…”

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confidence: 99%

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Continuous 3-year outdoor operation of a flat-panel membrane photobioreactor to treat effluent from an anaerobic membrane bioreactor

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A membrane photobioreactor (MPBR) plant was operated continuously for 3 years to evaluate the separate effects of different factors, including: biomass and hydraulic retention times (BRT, HRT), light path (Lp), nitrification rate (NOxR), nutrient loading rates (NLR, PLR) and others. The overall effect of all these parameters, which influence MPBR performance had not previously been assessed. The multivariate projection approach chosen for this study provided a good description of the collected data and facilitated their visualisation and interpretation. Forty variables used to control and assess MPBR performance were evaluated during three years of continuous outdoor operation by means of principal component analysis (PCA) and partial least squares (PLS) analysis. The PCA identified the photobioreactor light path as the factor with the largest influence on data variability. Other important factors were: nitrogen and phosphorus recovery rates (NRR, PRR), biomass productivity (BP), optical density of 680 nm (OD680), ammonium and phosphorus effluent concentration (NH 4 , P), HRT, BRT, air flow rate (F air) and nitrogen and phosphorus loading rates (NLR and PLR). The MPBR performance could be adequately estimated by a PLS model based on all the recorded variables, but this estimation 2 worsened appreciably when only the controlled variables (Lp, F air , HRT and BRT) were used as predictors, which underlines the importance of the non-controlled variables on MPBR performance. The microalgae cultivation process could thus only be partially controlled by the design and operating variables. A high nitrification rate was found to be inadvisable, since it showed an inverse correlation with NRR. In this respect, temperature and microalgae biomass concentration appeared to be the main factors to mitigate nitrifying bacteria activity.

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confidence: 67%

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confidence: 99%

Continuous 3-year outdoor operation of a flat-panel membrane photobioreactor to treat effluent from an anaerobic membrane bioreactor

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“…Generally, most of the nitrogen originating from sewage water is in the form of ammonium, nitrite or nitrates [49]. Initially, it is usually found that ammonium is converted into nitrite and then nitrate after a conventional biological treatment in the presence of nitrifying bacteria in a period of 24 h, or it can be consumed in the form of ammonium [50][51][52]. Once the nitrogen is in the form of nitrate, it can be uptaken by the microalgae to proceed to the bioremediation of the water [53].…”

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confidence: 99%

Cultivation of Microalgae and Cyanobacteria: Effect of Operating Conditions on Growth and Biomass Composition

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The purpose of this work is to define optimal growth conditions to maximise biomass for batch culture of the cyanobacterium Arthrospira maxima and the microalgae Chlorella vulgaris, Isochrysis galbana and Nannochloropsis gaditana. Thus, we study the effect of three variables on cell growth: i.e., inoculum:culture medium volume ratio (5:45, 10:40, 15:35 and 20:30 mL:mL), light:dark photoperiod (8:16, 12:12 and 16:8 h) and type of culture medium, including both synthetic media (Guillard’s F/2 and Walne’s) and wastewaters. The results showed that the initial inoculum:culture medium volume ratio, within the range 5:45 to 20:30, did not affect the amount of biomass at the end of the growth (14 days), whereas high (18 h) or low (6 h) number of hours of daily light was important for cell growth. The contribution of nutrients from different culture media could increase the growth rate of the different species. A. maxima was favoured in seawater enriched with Guillard’s F/2 as well as C. vulgaris and N. gaditana, but in freshwater medium. I. galbana had the greatest growth in the marine environment enriched with Walne’s media. Nitrogen was the limiting nutrient for growth at the end of the exponential phase of growth for C. vulgaris and N. gaditana, while iron was for A. maxima and I. galbana. The growth in different synthetic culture media also determines the biochemical composition of each of the microalgae. All species demonstrated their capability to grow in effluents from a wastewater treatment plant and they efficiently consume nitrogen, especially the three microalga species.

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“…Anaerobic membrane bioreactor (AnMBR) effluents have appeared to be ideal medium to enhance microalgae growth since they contain all the macro and micronutrients needed for microalgae growth and low amounts of solids and organic matter (Ruiz-Martínez et al, 2012). However, in large-scale outdoor microalgae cultivation systems, microalgae often coexist with other microorganisms that can act as competitors (Gonçalves et al, 2017;González-Camejo et al, 2019a). Since AnMBR effluents usually present high ammonium loads and low organic matter concentration (Seco et al, 2018), the competition between microalgae and ammonium oxidising bacteria (AOB) for ammonium uptake is likely to occur (Molinuevo-Salces et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria

et al. 2020

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Outdoor microalgae cultivation systems treating anaerobic membrane bioreactor (AnMBR) effluents usually present ammonium oxidising bacteria (AOB) competition with microalgae for ammonium uptake, which can cause nitrite accumulation. In literature, nitrite effects over microalgae have shown controversial results. The present study evaluates the nitrite inhibition role in a microalgae-nitrifying bacteria culture. For this purpose, pilot-and lab-scale assays were carried out. During the continuous outdoor operation of the membrane photobioreactor (MPBR) plant, biomass retention time (BRT) of 2 d favoured AOB activity, which caused nitrite accumulation. This nitrite was confirmed to inhibit microalgae performance. Specifically, continuous 5-d lab-scale assays showed a reduction in the nitrogen recovery efficiency by 32, 42 and 80% when nitrite concentration in the culture accounted for 5, 10 and 20 mg N•L-1 , respectively. On the contrary, short 30-min exposure to nitrite showed no significant differences in 2 the photosynthetic activity of microalgae under nitrite concentrations of 0, 5, 10 and 20 mg N•L-1. On the other hand, when the MPBR plant was operated at 2.5-d BRT, the nitrite concentration was reduced to negligible values due to increasing activity of microalgae and nitrite oxidising bacteria (NOB). This allowed obtaining maximum MPBR performance; i.e. nitrogen recovery rate (NRR) and biomass productivity of 19.7 ± 3.3 mg N•L-1 •d-1 and 139 ± 35 mg VSS•L-1 •d-1 , respectively; while nitrification rate (NOxR) reached the lowest value (13.5 ± 3.4 mg N•L-1 •d-1). Long BRT of 4.5 d favoured NOB growth, avoiding nitrite inhibition. However, it implied a decrease in microalgae growth and the accumulation of nitrate in the MPBR effluent. Hence, it seems that optimum BRT has to be within the range 2-4.5 d in order to favour microalgae growth with respect to AOB and NOB.

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Effect of ambient temperature variations on an indigenous microalgae-nitrifying bacteria culture dominated by Chlorella

Cited by 54 publications

References 48 publications

Continuous 3-year outdoor operation of a flat-panel membrane photobioreactor to treat effluent from an anaerobic membrane bioreactor

Continuous 3-year outdoor operation of a flat-panel membrane photobioreactor to treat effluent from an anaerobic membrane bioreactor

Cultivation of Microalgae and Cyanobacteria: Effect of Operating Conditions on Growth and Biomass Composition

Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria

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