Effect of Altered Sterol Composition on Growth Characteristics of
            <i>Saccharomyces cerevisiae</i>

Thompson, Elizabeth I.; Parks, Leo W.

doi:10.1128/jb.120.2.779-784.1974

Cited by 41 publications

(8 citation statements)

References 30 publications

(36 reference statements)

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“…One easily testable reaction is the transmethylation event that takes place in yeast mitochondria (20). We made a partially purified lipid-free preparation of this enzyme (21) and proceeded to test chemically synthesized (13) zymosteryl-oleate as a substrate. Because we have previously reported three separate enzyme activities for this enzyme (2), we tested all three with similar results.…”

Section: Resultsmentioning

confidence: 99%

Yeast sterol esters and their relationship to the growth of yeast

Bailey¹,

Parks²

1975

J Bacteriol

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Variation in the percentage of sterols esterified to long-chain fatty acids during cellular growth has been examined. Under all conditions, a constant percentage of sterol esters was maintained during exponential growth. This maintenance level was found to vary with different growth conditions. A sharp increase in the rate of esterification was observed upon entry of the culture into the stationary growth phase. The minor cellular sterol components were found to accumulate after this period of rapid sterol ester synthesis, with a relative decrease in the size of the ergosterol pool. Evidence is presented that sterol esters of ergosterol precursors are unable to be metabolized to ergosterol. Once esterified, the fatty acids do not appear to be scavenged during starvation conditions.

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Section: Resultsmentioning

confidence: 99%

Yeast sterol esters and their relationship to the growth of yeast

Bailey¹,

Parks²

1975

J Bacteriol

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“…Previous studies showed that another statin, lovastatin, caused a similar lowering of ergosterol levels (Lorenz & Parks, 1990; Baranova et al , 1996; Bibikova et al , 2004). The reduced ergosterol levels may lead to altered activities of numerous membrane‐localized enzymes (Thompson & Parks, 1974; McLean‐Bowen & Parks, 1981); however, in general, the effect of statins on mevalonate biosynthesis is expected to have broad consequences (Fig. 1).…”

Section: Discussionmentioning

confidence: 99%

Simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtDNA inCandida glabrata

Westermeyer

Macreadie

2007

FEMS Yeast Research

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Statins are widely used for lowering cholesterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase. Yeasts use HMG-CoA reductase for the same enzymatic step as humans, but in yeasts the main end-product of the pathway is ergosterol rather than cholesterol. We considered that insights into the effects of statins in humans could be gained by examination of the effects of simvastatin on the petite-positive yeast Candida glabrata. Simvastatin was found to inhibit growth, and this was associated with lower ergosterol levels. As simvastatin-treated cultures of yeast were passaged, the frequencies of petite cells (respiratory-deficient yeast mutants with deletions in the mitochondrial genome) increased with time and with simvastatin concentration. DNA staining of the petite mutants showed that they were devoid of mtDNA, suggesting a defect in the maintenance of mtDNA. These observations in C. glabrata may provide further insights into the molecular effects of statins in humans undergoing treatment for hypercholesterolemia. In addition, if C. glabrata is a valid model for studying statin treatments, it would be very useful for the preliminary screening of agents to reduce statin side-effects.

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“…More dramatic were the changes in the fatty acids of the phospholipids. The membranes of sterol mutants do appear, however, to have different properties than those of wild types (3,19,20,21,23,48,49…”

Section: Pcmentioning

confidence: 99%

Sterol methylation in Saccharomyces cerevisiae

McCammon¹,

Hartmann²,

Bottema³

et al. 1984

J Bacteriol

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Various nystatin-resistant mutants defective in S-adenosylmethionine: A24-sterol-C-methyltransferase (EC 2.1.1.41) were shown to possess alleles of the same gene, erg6. The genetic map location of erg6 was shown to be close to trpl on chromosome 4. Despite the single locus for erg6, S-adenosylmethionine: A24sterol-C-methyltransferase enzyme activity was found in three separate fractions: mitochondria, microsomes, and the "floating lipid layer." The amount of activity in each fraction could be manipulated by assay conditions. The lipids and lipid synthesis of mutants of Saccharomyces cerevisiae defective in the A24_

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Effect of Altered Sterol Composition on Growth Characteristics of Saccharomyces cerevisiae

Cited by 41 publications

References 30 publications

Yeast sterol esters and their relationship to the growth of yeast

Yeast sterol esters and their relationship to the growth of yeast

Simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtDNA inCandida glabrata

Sterol methylation in Saccharomyces cerevisiae

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