Sterol methylation in Saccharomyces cerevisiae

McCammon, Mark T.; Hartmann, Marie-Andrée; Bottema, C. D. K.; Parks, Leo W.

doi:10.1128/jb.157.2.475-483.1984

Cited by 62 publications

(13 citation statements)

References 46 publications

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“…Nevertheless, it was obvious that strains tend to group together either into set 1 or set 2. This gives support to the generalisation made by Holub & Lands 11 and McCammon et al 21 , that fatty acid composition of phospholipids is more susceptible to modification than is the class composition. Thus, in this study, inter-strain variation was less than interbatch variation because strains were so similar, except for the differences described above.…”

Section: Phosphatidylcholine Acyl Constituentssupporting

confidence: 76%

Polar Lipids of Brewer's Yeasts

Tosch

Geiger

Stretz

et al. 2005

Journal of the Institute of Brewing

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Both traditional and DNA-based methods sometimes fail to differentiate between closely related strains of commercial interest in the brewing industry. The aim of this study was to compare species and sub species of Saccharomyces cerevisiae on the basis of their polar lipid chemistry using chromatographic methods. Six isolates were studied after propagation under batch conditions. Polar lipids were then extracted from lyophilised cultures and analysed by TLC in order to separate phospholipid families. TLC showed that the major phospholipid classes present were PC > PE > PG. Two unidentified phospholipids were found, one only in strain 34 /70. The major peaks detected by GLC were identified as methyl esters of palmitic acid and palmitoleic acid. The fatty acid composition of PC varied between strains and novel data on lecithin acyl constituents were observed. The polar lipid method succeeded in differentiating strain 34 /70 -one of the most commonly used brewer's lager yeast -from strain 34 /78 and other species tested. The presence of unusual polar lipids in Saccharomyces sensu stricto yeasts may be useful in distinguishing between other closely related strains.

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Section: Phosphatidylcholine Acyl Constituentssupporting

confidence: 76%

Polar Lipids of Brewer's Yeasts

Tosch

Geiger

Stretz

et al. 2005

Journal of the Institute of Brewing

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“…This incompatibility with trpl is also a phenotype exhibited by erg6 mutants (Gaber et al, 1989). The erg6 mutants are defective for sterol methylation and therefore are unable to synthesize ergosterol, the major membrane sterol of yeast (McCammon et al, 1984). The altered membrane structure of these mutants results in a temperaturesensitive defect in the transport of tryptophan across the plasma membrane (Gaber et al, 1989).…”

Section: Resultsmentioning

confidence: 99%

Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway.

1993

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We have found that brefeldin A (BFA) inhibited the growth of an isel mutant of Saccharomyces cerevisiae. Genetic complementation and mapping studies demonstrated that isel was allelic to erg6, a gene required for the biosynthesis of the principal membrane sterol of yeast, ergosterol. Treatment of isel cells with BFA resulted in an immediate block in protein transport through the secretory pathway. Vacuolar carboxypeptidase Y (CPY) and the secreted pheromone a-factor accumulated as both the core glycosylated (ER) and al,6 mannosylated (early Golgi) forms in drugtreated cells. The modification of a-factor with al,6 mannose in BFA-treated cells did not appear to result from retrograde transport of the al,6 mannosyltransferase into the ER. We found that transport of CPY from medial and late Golgi compartments to the vacuole was unaffected by BFA, nor was secretion of al,3 mannosylated a-factor or invertase blocked by BFA. The effects of BFA on the secretory pathway were also reversible after brief exposure (<40 min) to the drug. We suggest that the primary effect of BFA in S.cerevisiae is restricted to the ER and the a(1,6 mannosyltransferase compartment of the Golgi complex.

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“…None of the previously characterized Erg-mutants generated through mutagenesis of a strain which is isogenic to the UPC20 and UPC27 parental showed the sterol composition of these Upc-strains. All of these Erg-mutants have very low, almost undetectable amounts of ergosterol (McCammon et al, 1984;Ramp, 1981).…”

Section: Resultsmentioning

confidence: 99%

Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism

Lewis

Keesler

Fenner

et al. 1988

Yeast

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Sterol uptake control mutants (upc-) have been isolated via ethylmethanesulfonate mutagenesis from wild-type Saccharomyces cerevisiae. These mutants are heme and sterol competent but possess the ability to accumulate exogenous sterol(s) under aerobic conditions. Previous studies demonstrate sterol uptake only in a hem-, erg- background; however, the Upc- strains described here are Hem+ and do not require exogenous sterol for growth. We were unable to obtain viable hem+, erg-, upc+ recombinants; such combinations appear to be lethal. Isolates of Upc mutants demonstrated different levels of sterol uptake, and sterol analysis revealed a broad phenotypic range with regard to amounts and accumulation of ergosterol and non-ergosterol sterols. Assays of acyl CoA: ergosterol acyltransferase and sterol ester hydrolase showed no apparent difference in activity between Upc mutants and the wild type.

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Sterol methylation in Saccharomyces cerevisiae

Cited by 62 publications

References 46 publications

Polar Lipids of Brewer's Yeasts

Polar Lipids of Brewer's Yeasts

Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway.

Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism

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