Effect of Alox-15 Polymorphism on GCF Levels of Lipoxin-A4 in Chronic Periodontitis: A Preliminary Study

Tarannum, Fouzia; Faizuddin, Mohamed

doi:10.1590/0103-6440201701094

Cited by 9 publications

(4 citation statements)

References 27 publications

(21 reference statements)

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“…In previous studies using GCF samples LXA4 level was reported to be lower in periodontitis, con rming that the ndings of decrease in salivary LXA4 might be an indicator of susceptibility to periodontitis [45][46][47]. Therefore, saliva level of LXA4 may be more reliable than serum in evaluating the its role in periodontitis.…”

Section: Discussionmentioning

confidence: 71%

Serum and Saliva Sirtuin 6, Lipoxin A4, Caspase8 Levels in Correlation with Periodontal Status in Severe Periodontitis

Çekici

Sahinkaya

Donmez

et al. 2022

Preprint

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Objectives: It has been stated that Sirtuin-6 (SIRT6) play a important role in regulation of inflammation, energy metabolism, homeostasis and apoptosis, and SIRT6 may be assosiciated with many diseases. The aim of this study was to evaluate saliva and serum SIRT6, Lipoxin A4 (LXA4) and Caspase-8 (CASP8) levels in correlation with periodontal clinical status in patients with periodontitis and healthy subjects. Materials and Methods: 20 patients with Stage III Grade B periodontitis (P) and 20 periodontally healthy individuals (control;C) were included in this study. Clinical periodontal parameters were measured. Saliva and serum levels of SIRT6, LXA4 and CASP8 were analyzed by enzyme-linked immunosorbent assay.Results: Serum SIRT6 and saliva LXA4 levels were significantly lower in the periodontitis group than in the control group (respectively; p=0.010, p= p:0.001). There was no statistically significant difference between the periodontitis and control groups for saliva SIRT6, serum LXA4, serum and saliva CASP8 levels (p>0.05). Significant negative correlations were found between all periodontal clinical parameters (PI, BOP, PPD, CAL) and saliva LXA4 level (p<0.05), and between PPD, CAL, and serum SIRT6 level (respectively; r=-0.465 and r=-0.473, p<0.05). Conclusions: This study demonstrated that significantly lower levels of serum SIRT6 and saliva LXA4 in periodontitis patients and their correlation with periodontal status. Detection of SIRT6 and saliva LXA4 might have a potential role for predicting periodontal status in the future with more precise sampling protocols and with better specifity in testing methods. Clinical Relevance: Serum SIRT6 and saliva LXA4 might be promising biomarkers for monitoring the susceptibility to periodontitis and predicting periodontal status.

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Section: Discussionmentioning

confidence: 71%

Serum and Saliva Sirtuin 6, Lipoxin A4, Caspase8 Levels in Correlation with Periodontal Status in Severe Periodontitis

Çekici

Sahinkaya

Donmez

et al. 2022

Preprint

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“…Macrophages and neutrophils also displayed slower kinetics and decreased maximal phagocytosis of P. gingivalis and Aggregatibacter actinomycetemcomitans in patients with LgAP compared with periodontally healthy controls 59 . While some studies have reported significant decreases in lipoxin A4 levels in the GCF of patients with periodontitis, 60,61 one study has reported higher serum lipoxin A4 levels in the periodontitis group compared with healthy controls ( p < .05) 42 . The demographic properties of the study population (ie, the severity of periodontitis; lifestyle habits), differences in samples (saliva, serum, and/or GCF), and methods of analysis (ie, ELISA, high‐performance liquid chromatography) could also explain the differences in study results.…”

Section: Discussionmentioning

confidence: 99%

Salivary levels of last generation specific pro‐resolving lipid mediators (SPMs) (protectin and maresin) in patients with cardiovascular and periodontal disease: A case‐control study

Önal

Fentoğlu

Aksoy

et al. 2021

J of Periodontal Research

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Background and Objective Periodontal disease and cardiovascular disease (CVD), which are both deemed to be triggered by inflammation, are recognized as public health problems. Evidence of host modulation via pro‐resolving lipid shown in previous studies supports a two‐way relationship between periodontitis and CVD. Last generation endogenous specific pro‐resolution lipid mediators (SPMs) such as protectins (PDs) and maresins (MaRs) may have potential effects on inflammatory pathogenesis via activation and resolution mechanisms. Currently, there are no data on SPM levels in patients with CVD and periodontal disease. We aimed to evaluate salivary levels of PD and MaR in patients with CVD and periodontal disease. Materials and Methods At total of 181 individuals comprising of 79 healthy controls (C) and 102 patients with diagnosed CVD were included cross‐sectionally. Unstimulated total salivary samples were obtained, and clinical periodontal parameters were determined. Salivary levels of PD and MaR were evaluated by ELISA. The periodontal status of the study population was classified as gingivitis (g) or periodontitis (p). Results Patients with CVD showed lower sociodemographic characteristics, increased clinical periodontal parameters (p < .05), decreased salivary PD (p < .001), and increased salivary MaR levels (p > .05). In the CVDg group, leukocyte, hemoglobin, hematocrit, and high‐density lipoprotein values were higher (p < .05). The CVDp group had a higher neutrophil‐to‐lymphocyte ratio (p < .05). While the PD level was highest in the Cg group, MaR was highest in the CVDp group. The salivary levels of PD and MaR were independent of other confounders in CVD and periodontal disease (p > .05). Conclusion(s) PDs and MaRs may play effective roles in pathogenesis associated with worsening cardiometabolic and periodontal status. These SPMs could also be predictors for conversion from a healthy (systemically and periodontally) to diseased state (CVD and/or periodontitis). Elucidation of the role of SPMs in the relationship between periodontal disease and CVD will enable the development of new host modulation strategies in the prevention and treatment of both diseases, and may also constitute an important public health step by increasing the quality of life of patients with CVD and periodontal disease.

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“…No significant association was detected between ALOX-15,c.-292 C > T polymorphism variants, and occurrence of NCFB which might pose questions about the genetic determinant’s role in disease pathogenesis. Few studies evaluated the effect of ALOX-15 gene polymorphism on LXA4 levels [ 35 ]. Also, its anti-inflammatory role was studied in animals [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Association between arachidonate lipoxygenase 15,c.-292 C > T gene polymorphism and non-cystic fibrosis bronchiectasis in children: a pilot study on the effects on airway lipoxin A4 and disease phenotype

Hussein,

Fouda,

Shehab

et al. 2024

Ital J Pediatr

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Background Persistent airway inflammation is a central feature of bronchiectasis. Arachidonate 15-lipoxygenase (ALOX-15) controls production of endogenous lipid mediators, including lipoxins that regulate airway inflammation. Mutations at various positions in ALOX-15 gene can influence airway disease development. We investigated association between ALOX-15,c.-292 C > T gene polymorphism and bronchiectasis unrelated to cystic fibrosis in Egyptian children. Also, lipoxin A4 (LXA4) level in bronchoalveolar lavage (BAL) was studied in relation to polymorphism genotypes and disease phenotypes determined by clinical, pulmonary functions, and radiological severity parameters. Methods This was an exploratory study that included 60 participants. Thirty children with non-cystic fibrosis bronchiectasis (NCFB) were compared with 30 age and sex-matched controls. ALOX-15,c.-292 C > T polymorphism was genotyped using TaqMan-based Real-time PCR. LXA4 was measured in BAL using ELISA method. Results There was no significant difference between patients and controls regarding ALOX-15,c.-292 C > T polymorphism genotypes and alleles (OR = 1.75; 95% CI (0.53–5.7), P = 0.35) (OR = 1; 95% CI (0.48-2), p = 1). BAL LXA4 level was significantly lower in patients, median (IQR) of 576.9 (147.6–1510) ng/ml compared to controls, median (IQR) of 1675 (536.8–2542) (p = 0.002). Patients with severe bronchiectasis had a significantly lower LXA4 level (p < 0.001). There were significant correlations with exacerbations frequency (r=-0.54, p = 0.002) and FEV1% predicted (r = 0.64, p = 0.001). Heterozygous CT genotype carriers showed higher LXA4 levels compared to other genotypes(p = 0.005). Conclusions Low airway LXA4 in children with NCFB is associated with severe disease phenotype and lung function deterioration. CT genotype of ALOX-15,c.-292 C > T polymorphism might be a protective genetic factor against bronchiectasis development and/or progression due to enhanced LXA4 production.

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Effect of Alox-15 Polymorphism on GCF Levels of Lipoxin-A4 in Chronic Periodontitis: A Preliminary Study

Cited by 9 publications

References 27 publications

Serum and Saliva Sirtuin 6, Lipoxin A4, Caspase8 Levels in Correlation with Periodontal Status in Severe Periodontitis

Serum and Saliva Sirtuin 6, Lipoxin A4, Caspase8 Levels in Correlation with Periodontal Status in Severe Periodontitis

Salivary levels of last generation specific pro‐resolving lipid mediators (SPMs) (protectin and maresin) in patients with cardiovascular and periodontal disease: A case‐control study

Association between arachidonate lipoxygenase 15,c.-292 C > T gene polymorphism and non-cystic fibrosis bronchiectasis in children: a pilot study on the effects on airway lipoxin A4 and disease phenotype

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