Effect of Aeration and Nutrients on Saccharomyces cerevisiae Cultivation using Lignocellulosic Hydrolysate from Empty Fruit Bunch

Hermansyah, Heri; Wisman, Adinda Putri; Firdaus, D Fitria Sari; Arbianti, Rita; Utami, Tania Surya; Kurnia, Annisa

doi:10.14716/ijtech.v6i7.1225

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…Lignocellulosic biomass has great potential as a low price raw material for the production of biofuels and high value products, such as bioethanol, enzyme and organic acid (Hermansyah et al, 2015;Hermansyah et al, 2018). One potential lignocellulose biomass is oil palm empty fruit bunch (EFB), since annually it is widely generated worldwide, especially in Indonesia, but unfortunately it has limited uses, only as an organic fertilizer or boiler fuel to generate electricity (Palamae et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Delignification of Oil Palm Empty Fruit Bunch using Peracetic Acid and Alkaline Peroxide Combined with the Ultrasound

Hermansyah¹,

Putri

Prasetyanto

et al. 2019

IJTech

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Lignocellulosic biomass has great potential as a low-cost source of fermentable sugar for the production of biofuels and high value organic acids. One potential biomass is oil palm empty fruit bunch, since it has high cellulose and hemicellulose content. However, its lignin content can hinder the access of cellulose and hemicellulose during the hydrolysis process. Therefore, an effective pretreatment for the delignification of lignocellulose biomass should be considered to reduce the lignin content. In this study, delignification of oil palm empty fruit bunch using peracetic acid and alkaline peroxide solution combined with the ultrasound method is investigated as a novel combination method of biomass pretreatment. The effect of pretreatment time was observed by using a peracetic acid solution for 1, 3, 5, 7 and 9 hours, followed by an alkaline peroxide solution for 4, 6, 8 and 10 hours. Based on the results, the best delignification was achieved by pretreatment using peracetic acid pretreatment for 3 hours, followed by alkaline peroxide pretreatment for 10 hours. Both pretreatments were assisted by the ultrasound method. The results show hemicellulose, cellulose and lignin content of 14.13%, 77.27% and 8.6% respectively. The lignin content was reduced by 68.73% and the cellulose content increased by 121.85%, relative to the untreated EFB. This result was considered as the best pretreatment, since the pretreatment time was shorter and high cellulose content together with low lignin content was achieved, which will improve the hydrolysis process.

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Section: Introductionmentioning

confidence: 99%

Delignification of Oil Palm Empty Fruit Bunch using Peracetic Acid and Alkaline Peroxide Combined with the Ultrasound

Hermansyah¹,

Putri

Prasetyanto

et al. 2019

IJTech

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“…OPEFB has been specifically used as feedstock for the production of levulinic acid and furfural; the highest yields were 52.1 mol% (C6 sugar basis) and 27.94 mol% (C5 sugar basis), respectively (Gozan, 2018). OPEFB can also be used as a carbon source for the growth medium of microorganisms like Saccharomyces cerevisiae to produce bioethanol with the highest yield: 24% (glucose basis) (Hermansyah, 2015).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Pretreatment Conditions for Microwave-assisted Alkaline Delignification of Empty Fruit Bunch by Response Surface Methodology

Harahap¹,

Rahman²,

Sadrina³

et al. 2019

IJTech

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Oil palm empty fruit bunch (OPEFB) is one of the prominent lignocellulosic wastes from the oil palm industry, and it has the potential for feedstock in biobased products. However, delignification must be applied. This study investigated the effect of microwave-assisted alkaline pretreatment of OPEFB by using response surface methodology with Box-Behnken design (BBD) to find the optimum pretreatment conditions. OPEFB (30-mesh) was treated with various concentrations of aqueous sodium hydroxide. The effect of three variables, microwave power (280840 W), NaOH concentration (13% w/v), and reaction time (39 min), was evaluated to improve lignin removal. The quadratic model indicated that microwave power of 832.9 W, NaOH concentration of 2.7% (w/v), and a reaction time of 8.9 min resulted in the highest lignin removal: 88.10%. FTIR and SEM analysis was also conducted on the untreated and treated OPEFB to evaluate the effectiveness of the pretreatment. These results showed that microwave-assisted alkaline pretreatment of OPEFB could effectively remove the lignin under a relatively short time period and low alkaline concentration.

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“…Indonesia has produced Empty Fruit Bunch (EFB) waste around 32 million tons per year from wide palm oil plantation [2]. Usually, EFB use for animal feed, while EFB is a potential material for bioethanol synthesis that in a pilot plant production of anhydrous ethanol using oil palm empty fruit bunch [3].…”

Section: Introductionmentioning

confidence: 99%

Characterization of yeast extract co-product bioethanol from empty palm oil bunch

et al. 2018

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One of the potential co-product of the manufacture of bioethanol made from oil palm empty fruit bunches are yeasts, which have antioxidant activity. Source of antioxidants can be obtained from vitamins and enzymes. In the characterization study of the antioxidant properties of yeast extract refers to a method performed by Xiong, by varying solvent and extraction time using hot water and ethanol 25%. From the variation of time and solvent extraction will be tested with the GSH content of alloxan method, to obtain the maximum concentration. The results show the isolation of amino acids with a hot solvent, at 15 minutes is better. These results are then carried out the characterization of amino acids with LCMS methods. An amino acid which has antioxidant activity is methionine, using the solvent water and tested methods of abortion LCMS and 3402.91 ppm ethanol with LCMS method implies 4137.002 ppm. Characterization of amino acids can then be used as a source of raw materials economic cosmetics worth selling.

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Effect of Aeration and Nutrients on Saccharomyces cerevisiae Cultivation using Lignocellulosic Hydrolysate from Empty Fruit Bunch

Cited by 6 publications

References 8 publications

Delignification of Oil Palm Empty Fruit Bunch using Peracetic Acid and Alkaline Peroxide Combined with the Ultrasound

Delignification of Oil Palm Empty Fruit Bunch using Peracetic Acid and Alkaline Peroxide Combined with the Ultrasound

Optimization of Pretreatment Conditions for Microwave-assisted Alkaline Delignification of Empty Fruit Bunch by Response Surface Methodology

Characterization of yeast extract co-product bioethanol from empty palm oil bunch

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