Effect of Administration with Low-dose FSH to Recipient Cows on Embryonic Survival after Bilateral Nonsurgical Embryo Transfer.

Kojima, Toshiyuki; Shimizu, Manabu; Tomizuka, Tsuneo

doi:10.1262/jrd.41.277

Cited by 1 publication

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“…The detailed results about embryo survival following the bilateral embryo transfers were reported elsewhere [15]. Briefly, no significant difference in embryonic survival rates was observed between the origin of embryos transferred.…”

Section: Methodsmentioning

confidence: 99%

Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos.

Kojima¹,

Akagi²,

Zeniya³

et al. 1996

Journal of Reproduction and Development

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Abstract. The aims of the present study were twofold. The first aim was to examine whether or not early pregnancy-associated thrombocytopenia (EPAT) occurred in cows following nonsurgical embryo transfer by observing the daily patterns of the concentration of peripheral platelets (PLT) during the preimplantation period of pregnancy. The other goal was to assess the use of PLT count as a diagnosis of early pregnancy in cows. In Experiment 1, out of 50 Holstein parous cows, 40 had one embryo nonsurgically transferred to each uterine horn on Day 6 or 7 (Day 0=onset of standing estrus). The remaining 10 cows served as controls having no embryos transferred. Blood was taken daily at the same time, from Day 6 or 7 following estrus (the day when the embryos were transferred in the non-controls) to the day of return to estrus for control cows and non-pregnant cows, or to Day 36 for pregnant cows. Bilateral embryo transfers resulted in 9 twin, 14 single, and 17 nonpregnancies. Some recipient cows showed an increase in PLT following embryo transfer, while others displayed a decrease in PLT. If significant PLT change was characterized either by a decrease or an increase of >20% compared to the value of pre-transfer, the proportions of cows indicating significant change were 78% (7/9) in twin, 64% (9/14) in single, 76% (13/17) in nonpregnancies and 30% (3/10) in controls. The proportions in twin (P<0.05), single (P<0.05) and nonpregnancies (P<0.01) were higher than that in controls. The effect of the particular group on PLT was significant (P<0.0025). In Experiment 2, each of several in vitro-produced blastocysts were nosurgically transferred to 9 Japanese Black cows on Day 7 or 8 following onset of estrus. Seven days after embryo transfer, an attempt to recover the previously transferred embryos by nonsurgical flushing was performed. Embryos could be recovered on Day 14 or 15 from 5 animals, whose blood was taken from the day of embryo transfer to the next day of embryo recovery and during the corresponding days of the following estrous cycle. For PLT, differences were detected between the both phases of 3 out of the 5 cows. The individual PLT variation in response means that EPAT does not necessarily occur in cows following embryo transfer, and that the use of PLT counts is unlikely to be clinically useful in assessing early pregnancy in cows. These results may suggest, however, that maternal platelet activation would occur during early pregnancy in cattle.

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Section: Methodsmentioning

confidence: 99%