“…Glucose (50%) was injected at time zero, through an intravenous catheter placed in the antecubital space at 0.3 g/kg body mass, and insulin (0.025 U/kg body mass) was injected at minute 20. Twenty-nine blood samples (at minutes 0, 2,3,4,5,6,8,10,12,14,16,19,22,23,24,25,27,30,40,50,60,70,80,90,100,120,140,160,180) were obtained, centrifuged, and stored at Ϫ80°C. Insulin was measured by immunoassay (Access Immunoassay System, Beckman Coulter, Fullerton, CA), and glucose with an oxidation reaction (YSI 2300, Yellow Springs, OH).…”