Effect of adiponectin secreted from adipose‐derived stem cells on bone‐fat balance and bone defect healing

Abstract

The efficacy of adiponectin (APN) in regulating bone metabolism remains controversial. This study aimed to investigate the role of APN secreted from adipose-derived stem cells on adipogenesis and osteogenesis. Human APN gene was transfected via recombinant adenovirus into adipose derived stem cells (ASCs) in vitro and were cocultured with bone marrow mesenchymal stem cells (BMSCs) in using a transwell chamber. Adipogenesis was inhibited in APN-transfected ASCs; in BMSCs, adipogenesis was inhibited, but osteogenesis was promoted in coculture with APN-transfected ASCs. Next, the same adenovirus construct was transfected into the abdominal adipose tissue of a Sprague Dawley rat in vivo, and then a tibia defect was established in the same rat. We confirmed there was higher gene and protein expression of APN in ASCs and the abdominal adipose tissue of these rat models. Development of adipocytes in abdominal adipose tissue was suppressed, and less new bone was formed in the bone defect area. In conclusion, APN secreted from ASCs could directly inhibit adipogenesis in ASCs and BMSCs and promote osteogenesis in the latter.However, APN overexpression in adipose tissue was inversely associated with bone formation in tibia defects potentially due to decreased levels of circulating boneactivating hormones.

Effect of Nanostructured Scaffold on Human Adipose-Derived Stem Cells: Outcome of In Vitro Experiments

“…ACRP30, also referred to as adiponectin, is the most abundant adipocytokine secreted by adipocytes and plays a pivotal role in glucose metabolism and energy homeostasis [ 34 , 35 ]. Although ACRP30 influences the proliferation of hASCs, it is also linked to adipocyte and osteoblast differentiation: an increase of ACRP30 mRNA expression and protein secretion have been observed in hASCs during their differentiation into osteoblast [ 36 , 37 , 38 ].…”

Poly(Glycerol Sebacate) in Biomedical Applications—A Review of the Recent Literature

“…Fabricated synthetic vascular grafts from PGS core and electrospun PCL shell have also been reported in several studies using a combination of electrospinning and salt leaching. [ 21,102,181,182 ] These acellular grafts degraded rapidly and supported the regeneration of neoarteries nearly free of foreign body reaction in rat abdominal aorta. [ 21 ] To enhance mechanical properties suitable for human application, the thickness of the PCL sheath was increased, which significantly reinforced the whole grafts.…”

“…Later, the superiority of PGS/PCL grafts over autologous jugular vein grafts was observed regarding the reconstruction of the carotid artery. [ 182 ] The comparison showed that PGS/PCL grafts were remodeled into neoarteries with smooth and even vessel wall comparable to native carotid arteries within 12 months after implantation, whereas autologous grafts remodeled into neoarteries with dilated vessel cavity and thickening of the vessel wall. Additionally, in PGS/PCL neoarteries, muscular components were well organized, and fibers with higher flexibility and elasticity were observed.…”

An adiponectin receptor agonist promote osteogenesis via regulating bone‐fat balance

“…Immunoblots were performed as we previously described. 2 Treated cell samples were lysed with RIPA lysis buffer for around 30 min on ice. Antibodies used for immunoblot were listed in Table S2.…”