Background: Pantja is a dual purpose indigenous goat breed, reared in Tarai region of Uttarakhand and Uttar Pradesh. There is a lack of thorough study about the seminal characteristics of this goat breed. Hence, the present study was conducted to evaluate the level of certain biochemical constituents and their relationship with macroscopical and microscopical semen characteristics in Pantja buck.
Methods: A total thirty two ejaculates from four sexually mature Pantja buck were analysed. The ejaculates were evaluated for volume (0.36±0.06 ml), mass activity (4.47±0.22), concentration (3.54±0.13 x 109/ml), individual motility (85.28±1.17%), viability (88.28±0.78%), morphological abnormalities (4.70±0.17%), plasma membrane integrity (86.31±0.62%) and acrosome integrity (94.31±1.05%) by standard semen evaluation protocol. Seminal plasma ALT (18.10±0.45 U/L), AST (124.04±0.86 U/L), ALP (49.98±0.38 U/L), LDH (216.36±1.78 U/L), GSH-Px (10.90±0.21 U/ml), MDA (2.14±0.05 nmol/ml), albumin (2.29±0.03 gm/dl), globulin (2.52±0.02 gm/dl), total proteins (4.81±0.05 gm/dl), calcium (11.07±0.18 mg/dl) and phosphorus (12.34±0.20 mg/dl) were determined spectrophotometrically. Two tailed Pearson’s correlation was applied to check the relationship among the above attributes.
Result: A significant correlation was observed among the biochemical constituents of seminal plasma and seminal attributes of Pantja bucks. The selected biochemical constituents also showed significant correlation with each other.