Effect of activated protein C in second intention healing of equine distal limb wounds: a preliminary study

Bischofberger, Andrea S; Tsang, AS; Horadagoda, N. U.; Dart, CM; Perkins, N. R.; Jeffcott, L. B.; Jackson, Chris; Dart, Andrew

doi:10.1111/avj.12363

Cited by 17 publications

(12 citation statements)

References 33 publications

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“…Most experimental studies of equine distal limb wound healing using surgical models have been performed on forelimbs. [1][2][3][4][5][6][7][28][29][30][39][40][41][42] However, the prolonged healing times in this study are consistent with the results of the only other study to investigate distal limb wound healing in equine hindlimbs. 43 That study showed that hindlimb wounds healed significantly slower than forelimb wounds, but the pattern of healing is similar, with wounds initially retracting, followed by a period of consistent and steady contraction.…”

Section: Discussionsupporting

confidence: 84%

Comparison of the effects of topical application of UMF20 and UMF5 manuka honey with a generic multifloral honey on wound healing variables in an uncontaminated surgical equine distal limb wound model

et al. 2017

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Section: Discussionsupporting

confidence: 84%

Comparison of the effects of topical application of UMF20 and UMF5 manuka honey with a generic multifloral honey on wound healing variables in an uncontaminated surgical equine distal limb wound model

et al. 2017

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“…Numerous studies have highlighted the capacity of honey in healing chronic wounds in humans and animals [ 28 , 29 , 30 , 31 ]. This property can be related to the antibacterial capacity of honey and other mechanisms related to its physical properties, such as pH, and to its immunostimulatory and anti-inflammatory effects [ 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts

Giampieri

Gasparrini

Forbes-Hernández

et al. 2018

IJMS

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The antioxidant capacity and the phytochemical composition of two by-products from beeswax recycling processes were recently investigated. The aim of the present work was to evaluate the efficacy of one of these by-products, MUD1, against the oxidative stress induced by 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) in human dermal fibroblast (HDF) cells. After a preliminary viability assay, the protective effect of MUD1 was investigated through the measurement of apoptosis level, the reactive oxygen species (ROS) and nitrite (NO2−) production, the level of protein and lipid biomarkers (carbonyl groups, total glutathione and thiobarbituric acid-reactive substance) of oxidative damage, and the measurement of antioxidant enzymes activities (glutatione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and catalase). The obtained results showed that MUD1 exerted protective effects on HDF, increasing cell viability and counteracted the oxidative stress promoted by AAPH-treatment, and improved mitochondria functionality and wound healing capacities. This work shows the antioxidant effects exerted by beeswax by-products, demonstrating for the first time their potential against oxidative stress in human dermal fibroblast cells; however, further research will be necessary to evaluate their potentiality for human health by more deeply in vitro and in vivo studies.

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“…APC, a serine protease, is considered to play an important role in the maintenance of hemostasis (21), inhibitions of cytokines release, and reduction of leucocyte recruitment (22). In the past years, several studies have recognized that APC has an anti-inflammatory effect that is beneficial for stroke (23), sepsis (24), ischemia-reperfusion injury (25) in humans.…”

Section: Discussionmentioning

confidence: 99%

Activated protein C inhibits lung injury induced by LPS via downregulating MAPK signaling

Zhou

Jing

et al. 2018

Exp Ther Med

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The aim of the present study was to investigate the effect and the underlying mechanism of activated protein C (APC) in lipopolysaccharide (LPS) induced lung injury, as well as the potential mechanism. According to the treatment, 50 rats were randomly divided into 5 groups: Control, model (LPS), low-dose group [LPS + 0.1 mg/kg recombined human activated protein C (rhAPC)], median-dose group (LPS + 0.3 mg/kg rhAPC) and high-dose group (LPS + 0.5 mg/kg rhAPC). Then, inflammation in the lung was assessed using hematoxylin and eosin (H&E) staining. Following the collection of bronchoalveolar lavage fluid (BALF), the number of leukocytes and neutrophils in BALF was counted, and superoxide dismutase (SOD) activity was assessed, as well as the expression levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α using ELISA. Subsequently, the expression and phosphorylation of P-38, extracellular signal-regulated kinase (Erk)-1/2, and c-Jun N-terminal kinase (JNK) were estimated using western blotting. Based on H&E staining, rhAPC markedly suppressed inflammatory infiltration in the lung induced by LPS in a dose-dependent manner. In addition, rhAPC also significantly attenuated the accumulation of leptocytes and neutrophils, and the reduction of SOD in BALF induced by LPS in a dose-dependent manner. rhAPC also significantly attenuated the elevation of IL-1β, IL-6 and TNF-α in BALF induced by LPS in a dose-dependent manner. Further mechanistic analysis revealed that rhAPC treatment could evidently attenuate the phosphorylation levels of P-38, Erk1/2 and JNK in the lung induced by LPS in a dose-dependent manner. In conclusion, APC significantly alleviated the lung inflammation induced by LPS by downregulating the phosphorylation of P-38, ERK1/2 and JNK.

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Effect of activated protein C in second intention healing of equine distal limb wounds: a preliminary study

Abstract: The improvements in histological scores in APC-treated wounds suggest further study into the effect of APC on second intention wound healing in horses is warranted.

Cited by 17 publications

References 33 publications

Comparison of the effects of topical application of UMF20 and UMF5 manuka honey with a generic multifloral honey on wound healing variables in an uncontaminated surgical equine distal limb wound model

Comparison of the effects of topical application of UMF20 and UMF5 manuka honey with a generic multifloral honey on wound healing variables in an uncontaminated surgical equine distal limb wound model

Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts

Activated protein C inhibits lung injury induced by LPS via downregulating MAPK signaling

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