Background: Disturbing dyspeptic complaints may be seen in the use of milk and dairy products in people with lactose intolerance. Lactose-free milk and dairy products are produced for people with these complaints. The widespread use of activated carbon for dyspeptic complaints can also be used for adsorbing lactose. Methods: For this purpose, the binding energy of lactase to lactose was studied in silico, lactose adsorption enthalpic changes of activated carbon were calculated by HPLC, plain and activated carbon yoghurt was produced and glucose+galactose and lactose levels were determined in yoghurts. The effects of these yogurts on serum glucose levels were compared in mice. Results: In silico studies, the affinity of lactase with lactose was found to be -7.12 kcal/mol. It was determined by HPLC that activated carbon adsorbed lactose with an energy of -1,785 kcal/mol, and glucose+galactose levels and lactose ratios were lower in yogurt with added activated carbon. It was determined that there was no change in serum glucose levels in the 45th and 90th minutes following fasting in the mice fed with activated carbon yogurt compared to the mice fed plain yogurt. Conclusion: Yogurt with activated carbon can be an alternative diet for individuals with lactose intolerance, by converting lactose to lactase in the presence of lactase and adsorbing lactose in the absence of lactose.