Effect of acetoacetyl coa thiolase amplification on sterol synthesis in the yeasts S. cerevisiae and S. uvarum

Dequin, Sylvie; Boutelet, F; Servouse, Madeleine; Karst, Francis

doi:10.1007/bf01027056

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…Moreover, ergosterol accumulation in these cells was no greater than that in control cultures, indicating that the overexpression of phosphomevalonate kinase had little effect on the synthesis of this isoprene-derived product. Similar findings were previously reported in studies in which the activity of acetoacetyl CoA thiolase (16) or HMG CoA reductase (56) was increased tenfold in yeast strains bearing the corresponding structural gene on a multicopy plasmid. From these studies and our own, it seems that yeast sterol production may be regulated primarily through other ratelimiting enzymes of sterol biosynthesis such as HMG CoA synthetase or squalene synthetase.…”

Section: Discussionsupporting

confidence: 90%

Cloning and Characterization of ERG8 an Essential Gene of Saccharomyces cerevisiae That Encodes Phosphomevalonate Kinase

Tsay

Robinson

1991

Molecular and Cellular Biology

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Saccharomyces cerevisiae strains that contain the ery8-1 mutation are temperature sensitive for growth due to a defect in phosphomevalonate kinase, an enzyme of isoprene and ergosterol biosynthesis. A plasmid bearing the yeast ERG8 gene was isolated from a YCp50 genomic library by functional complementation of the erg8-1 mutant strain. Genetic analysis demonstrated that integrated copies of an ERG8 plasmid mapped to the erg8 locus, confirming the identity of this clone. Southern analysis showed that ERG8 was a single-copy gene. Subcloning and DNA sequencing defined the functional ERG8 regulon as an 850-bp upstream region and an adjacent 1,272-bp open reading frame. The deduced 424-amino-acid ERG8 protein showed no homology to known proteins except within a putative ATP-binding domain present in many kinases. Disruption of the chromosomal ERG8 coding region by integration of URA3 or HIS3 marker fragments was lethal in haploid cells, indicating that this gene is essential. Expression of the ERG8 gene in S. cerevisiae from the galactose-inducible galactokinase (GAL1) promoter resulted in 1,000-fold-elevated levels of phosphomevalonate kinase enzyme activity. Overproduction of a soluble protein with the predicted 48-kDa size for phosphomevalonate kinase was also observed in the yeast cells.

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Section: Discussionsupporting

confidence: 90%

Cloning and Characterization of ERG8 an Essential Gene of Saccharomyces cerevisiae That Encodes Phosphomevalonate Kinase

Tsay

Robinson

1991

Molecular and Cellular Biology

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show abstract

“…Similar findings were previously reported in studies in which the activity of acetoacetyl CoA thiolase (16) or HMG CoA reductase (56) was increased tenfold in yeast strains bearing the corresponding structural gene on a multicopy plasmid. From these studies and our own, it seems that yeast sterol production may be regulated primarily through other ratelimiting enzymes of sterol biosynthesis such as HMG CoA synthetase or squalene synthetase.…”

Section: Resultssupporting

confidence: 78%

“…of erg8 strains cosegregated, suggesting that both traits were the result of a single genetic defect. erg8 strains were recently used to explore the regulation of ergosterol metabolism in S. cerevisiae (16,47), but no detailed characterization of the yeast gene for phosphomevalonate kinase has been reported.…”

mentioning

confidence: 99%

Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase.

Tsay¹,

Robinson²

1991

Mol. Cell. Biol.

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Saccharomyces cerevisiae strains that contain the erg8-1 mutation are temperature sensitive for growth due to a defect in phosphomevalonate kinase, an enzyme of isoprene and ergosterol biosynthesis. A (10,24,33,50). This enzyme functions in the elaboration of isoprene subunits, which are used for the synthesis of a variety of essential compounds including sterols, dolichols, and ubiquinones. Isoprene-derived molecules are also used in some species for the covalent modification of tRNAs (17) and specific proteins, including ras and a-factor in S. cerevisiae (1,27,46 (12,47,53). Some evidence suggests that in both yeast and mammalian cells, significant pathway regulation may be mediated through additional enzymes of sterol biosynthesis, including mevalonate kinase and squalene synthetase (18,20,52

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Some Properties of Enzymes Involved in the Biosynthesis and Metabolism of 3-Hydroxy-3-Methylglutaryl-CoA in Plants

Bach

Weber

Motel

1990

Biochemistry of the Mevalonic Acid Pathway to Terpenoids

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Effect of acetoacetyl coa thiolase amplification on sterol synthesis in the yeasts S. cerevisiae and S. uvarum

Cited by 5 publications

References 11 publications

Cloning and Characterization of ERG8 an Essential Gene of Saccharomyces cerevisiae That Encodes Phosphomevalonate Kinase

Cloning and Characterization of ERG8 an Essential Gene of Saccharomyces cerevisiae That Encodes Phosphomevalonate Kinase

Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase.

Some Properties of Enzymes Involved in the Biosynthesis and Metabolism of 3-Hydroxy-3-Methylglutaryl-CoA in Plants

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