Effect of Acetate and Carbonate Buffers on the Photolysis of Riboflavin in Aqueous Solution: A Kinetic Study

Ahmad, Iqbal; Anwar, Zubair; Iqbal, Kefi; Ali, Syed Abid; Mirza, Tania; Khurshid, Adeela; Khurshid, Aqeela; Arsalan, Adeel

doi:10.1208/s12249-013-0067-6

Cited by 16 publications

(16 citation statements)

References 47 publications

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“…RF is known to degrade to FMF and LC in acid solution and to FMF, LC and LF in alkaline solution on exposure to light . FMF is an intermediate product leading to the formation of LC and LF in these reactions .…”

Section: Resultsmentioning

confidence: 99%

“…Riboflavin (vitamin B 2 ), (RF), is a highly fluorescent compound and exhibits an intense yellow‐green fluorescence in aqueous solution with emission in the 520 to 530 nm region on excitation at 444 nm. The fluorescence of RF is quenched by acid and alkali, metal ions, aliphatic and aromatic compounds and buffer anions . The fluorescence characteristics have been used as a basis for the assay of RF.…”

Section: Introductionmentioning

confidence: 99%

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Stability‐indicating spectrofluorimetric method for the assay of riboflavin and photoproducts: Kinetic applications

et al. 2018

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A stability-indicating spectrofluorimetric method has been developed for the simultaneous assay of riboflavin (RF) and photoproducts, formylmethylflavin (FMF), lumichrome (LC) and lumiflavin (LF) in aqueous solution. The method is based on the extraction of LC formed in acid solution and LC and LF formed in alkaline solution with chloroform at pH 2.0 and their assay by fluorescence measurements at 478 and 530 nm, respectively. The aqueous phase, on readjustment of the pH to 6.5, is used to extract FMF with chloroform and its assay is carried out at 530 nm. The aqueous phase is then used for the assay of RF at 530 nm. The proposed method gives more accurate results for the assay of RF compared to those of the United States Pharmacopeia (USP) spectrofluorimetric method which does not take into account the presence of RF photoproducts having similar fluorescence characteristics. The proposed method along with the USP method has been applied to the study of the kinetics of photolysis of RF, assay of stored commercial vitamin preparations and their radiated samples. The results show that the USP method does not distinguish between the fluorescence of RF and its photoproducts, and, therefore, gives erroneous results with about 11% excess in the quantity of the vitamin compared to that of the proposed method. This is due to the interference of the fluorescence of photoproducts in the assay of RF. The method has been validated for various analytical parameters according to the guideline of the International Council for Harmonization (ICH).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stability‐indicating spectrofluorimetric method for the assay of riboflavin and photoproducts: Kinetic applications

et al. 2018

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“…An increase in RF photodegradation was also observed when its solution was irradiated by visible light with a herbicide, 2,4-dichlorophenoxyacetic acid, in the presence of Britton–Robinson buffer at pH 6 [90]. Similarly, a catalytic effect has also been noted in acetate and carbonate buffers for RF where a change in rate of degradation was observed with an increase in pH [88]. …”

Section: Reviewmentioning

confidence: 99%

Photo, thermal and chemical degradation of riboflavin

Sheraz¹,

Kazi²,

Ahmed³

et al. 2014

Beilstein J. Org. Chem.

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199

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SummaryRiboflavin (RF), also known as vitamin B2, belongs to the class of water-soluble vitamins and is widely present in a variety of food products. It is sensitive to light and high temperature, and therefore, needs a consideration of these factors for its stability in food products and pharmaceutical preparations. A number of other factors have also been identified that affect the stability of RF. These factors include radiation source, its intensity and wavelength, pH, presence of oxygen, buffer concentration and ionic strength, solvent polarity and viscosity, and use of stabilizers and complexing agents. A detailed review of the literature in this field has been made and all those factors that affect the photo, thermal and chemical degradation of RF have been discussed. RF undergoes degradation through several mechanisms and an understanding of the mode of photo- and thermal degradation of RF may help in the stabilization of the vitamin. A general scheme for the photodegradation of RF is presented.

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“…The photolysis of RF in aqueous solution (3,8,57) and in organic solvents (36,57) follows first-order kinetics. A kinetic plot for the photolysis of RF in methanol (Fig.…”

Section: Kinetics Of Photolysismentioning

confidence: 99%

Solvent Effect on the Photolysis of Riboflavin

et al. 2015

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Abstract. The kinetics of photolysis of riboflavin (RF) in water (pH 7.0) and in organic solvents (acetonitrile, methanol, ethanol, 1-propanol, 1-butanol, ethyl acetate) has been studied using a multicomponent spectrometric method for the assay of RF and its major photoproducts, formylmethylflavin and lumichrome. The apparent first-order rate constants (k obs ) for the reaction range from 3.19 (ethyl acetate) to 4.61×10

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Effect of Acetate and Carbonate Buffers on the Photolysis of Riboflavin in Aqueous Solution: A Kinetic Study

Cited by 16 publications

References 47 publications

Stability‐indicating spectrofluorimetric method for the assay of riboflavin and photoproducts: Kinetic applications

Stability‐indicating spectrofluorimetric method for the assay of riboflavin and photoproducts: Kinetic applications

Photo, thermal and chemical degradation of riboflavin

Solvent Effect on the Photolysis of Riboflavin

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