Effect of a vitamin D₃-based nutritional supplement (`Videchol') on carbohydrate metabolism of rats following chronic low dose-rate irradiation

Abstract

In this study we evaluated the effects of the administration of a vitamin D3 preparation 'Videchol' to chronically irradiated rats (1 cGy day(-1)) by the assessment of the activities of several glycolytic enzymes: lactic dehydrogenase (LDH) (EC 1.1.1.28), pyruvate kinase (PK) (EC 2.7.1.40) and hexokinase (HK) (EC 2.7.1.1), in populations of erythroid and myeloid bone marrow cells. Videchol treatment of irradiated rats led to the normalisation of HK and LDH activity at cumulative doses of around 30 cGy in granulocyte-monocyte cells and to normalisation of LDH and PK activities in erythroid cells starting at 20 cGy in comparison with irradiated rats who did not receive Videchol. The reaction kinetic parameters of LDH in erythrocytes changed according to the redistribution pattern of the isozymes throughout the different stages of the experiment. The administration of Videchol to irradiated rats led to a rearrangement of the LDH isozymes ratio characterised by kinetic properties more comparable to those of the controls. Thus, vitamin D3 appears to induce a normalisation of carbohydrate metabolism in rats chronically irradiated with low dose-rate ionising radiation.

Vitamin D does not improve the metabolic health of patients with chronic kidney disease stage 3–4: A randomized controlled trial

“…Starikovich et al . demonstrated that the use of cholecalciferol was able to improve aspects of the glycolytic pathway in an experimental model and thus this may be an avenue for future research to explore.…”

“…7 Additionally, in models of vitamin D deficiency there is a significant impairment of the tricarboxylic acid cycle, 46 which taken together has implications on the oxidative potential of the individuals in the current study. Starikovich et al demonstrated that the use of cholecalciferol was able to improve aspects of the glycolytic pathway in an experimental model 47 and thus this may be an avenue for future research to explore. The reduction in adiponectin was unexpected, especially in view of no change in factors known to influence circulating levels.…”

Ionizing Radiation as a Source of Oxidative Stress—The Protective Role of Melatonin and Vitamin D

“…However, the analysis of the available literature indicates very limited research data on the radioprotective role of vitamin D in the context of IR action. It was observed that the administration of vitamin D3 (0.7 μg of vitamin D3 or 28 IU/100 g body mass) to chronically irradiated Wistar rats (0.01 Gy per day for 30 days) induced the normalization of carbohydrate metabolism by improving the activities of glycolytic enzymes in erythroid and myeloid bone marrow cells [180]. In the in vitro study of Müller et al [181], it was found that the cell growth and clonogenic survival of irradiated keratinocytes (cell line HaCaT), pretreated with calcitriol, were significantly increased when compared to the untreated cells after IR As with melatonin, vitamin D and its analogues have been found to selectively radiosensitize cancer cells, including breast and non-small cell lung tumor cells [185][186][187][188][189], which makes it a promising adjuvant in radiotherapy, enhancing the treatment effect and reducing side effects.…”