Effect of a trehalase inhibitor, validoxylamine A, on the oocyte development and ootheca formation in Blattella germanica and Periplaneta fuliginosa

義明, 河野; 正和, 高橋; 和弘, 松下; 正実, 仁科; 幸彦, 亀田

doi:10.7601/mez.50.33_1

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…In B. germanica , a parallel relationship between hemolymph trehalose levels and ovarian maturation was observed, suggesting that trehalose supplies the energy demand required by the reproductive cycle (Huang and Lee, 2011). Moreover, injection with a highly specific trahalase inhibitor, validoxylamine A (VAA) suppressed Vg synthesis in the fat body and its uptake by the maturating oocytes, as has been demonstrated in several insect species (Tanaka et al, 1998; Kono et al, 1999). In the cockroach P. americana , inhibition of the trehalase activity resulted in a reduced Vg accumulation in the ovary, which suggests that the uptake of Vg by developing oocytes is an energy-demanding process (Kono et al, 2001).…”

Section: Discussionmentioning

confidence: 79%

Adipokinetic Hormone Receptor Mediates Trehalose Homeostasis to Promote Vitellogenin Uptake by Oocytes in Nilaparvata lugens

et al. 2019

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Adipokinetic hormones (AKHs) are well known to mobilize lipids and carbohydrates for energy-consuming activities in insects. These neuropeptides exert their functions by interacting with AKH receptors (AKHRs) located on the plasma membrane of fat body cells, which regulates energy mobilization by stimulating lipolysis of triacylglycerols (TAG) to diacylglycerols (DAG) and conversion of glycogen into trehalose. Here, we investigated the roles of AKH/AKHR signaling system in trehalose metabolism and vitellogenesis during female reproduction in the brown planthopper, Nilaparvata lugens. Knockdown of AKHR expression by RNA interference (RNAi) resulted in a decrease of the circulating trehalose in hemolymph and significantly increased levels of two trehalases in fat bodies, indicating that the modulation of hemolymph trehalose levels by AKHR may be mediated by regulating trehalose degradation. In addition, adult females that had been injected with double-stranded RNA (dsRNA) for AKHR exhibited delayed oocyte maturation, prolonged pre-oviposition period, as well as decline in egg number and reduction in fecundity. Considering that these phenotypes resulting from AKHR silencing are similar to those of vitellogenin receptor (VgR) RNAi, we further analyzed a possible connection between AKHR and vitellogenesis. Knockdown of AKHR showed no effects on the Vg synthesis in fat bodies, whereas it significantly reduced the levels of VgR in ovaries. With RNAi-females, we observed an increase of Vg accumulation in hemolymph and a decrease of Vg deposition in ovaries. Moreover, the decrease in VgR expression and Vg incorporation by developing oocytes could be partially rescued by injection of trehalose into AKHR RNAi females. The present study has implicated trehalose in the AKH/AKHR signaling-mediated control of reproduction and provided new insight into mechanisms of AKH/AKHR regulation of trehalose metabolism in insect vitellogenesis, oocyte maturation and fecundity.

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Section: Discussionmentioning

confidence: 79%

Adipokinetic Hormone Receptor Mediates Trehalose Homeostasis to Promote Vitellogenin Uptake by Oocytes in Nilaparvata lugens

et al. 2019

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“…Currently, hemolymph components of insects are measured by 1 H-NMR (Thompson, 1990;Kono et al, 1993Kono et al, , 1994Kono et al, , 1999aTakahashi et al, 1995;Phalaraksh et al, 1999;Lenz et al, 2001). NMR analysis has made it possible to determine the concentrations of sugars in a small sample and with minimal pretreatment.…”

Section: Introductionmentioning

confidence: 99%

High concentrations of trehalose in aphid hemolymph

Moriwaki

Matsushita

Nishina

et al. 2003

Appl. Entomol. Zool.

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The major components of hemolymph were examined by 1 H-NMR in seven species of aphids, Acyrthosiphon pisum, Aphis gossypii, Aphis sambuci, Lachnus tropicalis, Megoura crassicauda, Myzus persicae and Uroleucon nigrotuberculatum. Trehalose was detected as the main component from these aphids, and its concentrations in these insects were much higher (196 mM in L. tropicalis to 926 mM in A. gossypii) than those of two other homopterous insects, Cryptotympana facialis (91 mM) and Nephotettix cincticeps (53 mM). Concentrations in L. tropicalis and A. gossypii were equivalent to 1.6% to 2.0% of the fresh weight. Glucose appeared to be an artifact in the hemolymph, because it was not found in the hemolymph when validoxylamine A, a trehalase inhibitor, was added.

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“…Additionally, the injection of validoxylamine A (VAA), which is a specific trehalase inhibitor, suppresses oocyte development in the American cockroach, Periplaneta americana . Meanwhile, ovary, accessory gland, and fat body trehalase levels in cockroaches decrease considerably in response to an injection of VAA (Tanaka et al ., 1998; Kono et al ., 1999). The observed increase in hemolymph trehalose contents is indicative of the inhibition of trehalose metabolism in the examined tissues.…”

Section: Discussionmentioning

confidence: 99%

Phospholipase C gamma (PLCγ) regulates soluble trehalase in the 20E‐induced fecundity of Apolygus lucorum

et al. 2020

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Apolygus lucorum is the dominant pathogenic insect attacking Bacillus thuringiensis (Bt) cotton in China. Additionally, 20-hydroxyecdysone (20E) has important functions in many biological processes, including insect reproduction. Phospholipase C (PLC), which is an essential enzyme for phosphoinositide metabolism, is involved in 20E signal transduction, but its function in 20E-mediated reproduction in A. lucorum remains unclear. In this study, 20E increased AlPLCγ transcription as well as the abundance and activity of the encoded protein during molting and metamorphosis. The 20E treatment also induced the considerable accumulation of two second messengers, inositol triphosphate and diacylglycerol. The expression levels of genes encoding vitellogenin (AlVg) and soluble trehalase (AlTre-1) were similar to those of AlPLCγ , and were upregulated in response to 20E. The silencing of AlPLCγ resulted in downregulated expression of AlTre-1 and AlVg. However, the silencing of AlTre-1 and AlVg did not affect AlPLCγ expression. Moreover, the silencing of AlVg did not alter AlTre-1 expression. Furthermore, an examination of the insect specimens indicated that AlPLCγ is required for female adult reproduction, and that downregulated expression of this gene is associated with decreases in fecundity, adult longevity, and egg hatching rate as well as delayed oocyte maturation. We propose that 20E regulates AlTre-1 expression via AlPLCγ and affects Vg expression as well as ovary development to facilitate the reproductive activities of A. lucorum females.

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Effect of a trehalase inhibitor, validoxylamine A, on the oocyte development and ootheca formation in Blattella germanica and Periplaneta fuliginosa

Abstract: ： Effect of a potent trehalase inhibitor ， validoxylamine A (VAA) ， on the oocyte development and ootheca formation was observed in two species of cockrQaches

Cited by 10 publications

References 14 publications

Adipokinetic Hormone Receptor Mediates Trehalose Homeostasis to Promote Vitellogenin Uptake by Oocytes in Nilaparvata lugens

Adipokinetic Hormone Receptor Mediates Trehalose Homeostasis to Promote Vitellogenin Uptake by Oocytes in Nilaparvata lugens

High concentrations of trehalose in aphid hemolymph

Phospholipase C gamma (PLCγ) regulates soluble trehalase in the 20E‐induced fecundity of Apolygus lucorum

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