Effect of a Standardised Dietary Restriction Protocol on Multiple Laboratory Strains of Drosophila melanogaster

Grandison, Richard C.; Wong, Richard; Bass, Timothy M.; Partridge, Linda; Piper, Matthew D. W.

doi:10.1371/journal.pone.0004067

Cited by 98 publications

(112 citation statements)

References 46 publications

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“…In previous studies, methionine was often experimentally restricted in relation to other amino acids, and a lifespanextending effect was commonly observed in rodents (Miller et al 2005), but not in fruit flies (Troen et al 2007). More recently, methionine was found to be the only amino acid that, when added to a restricted D. melanogaster diet, could restore fecundity and simultaneously maintain lifespan at the level observed under DR (Grandison et al 2009b). Given that this finding is correct, it implies that the trade-off between lifespan and fecundity can be decoupled by environmental manipulation.…”

Section: Introductionmentioning

confidence: 98%

Interactive effects of sex, social environment, dietary restriction, and methionine on survival and reproduction in fruit flies

Zajitschek

Friberg

et al. 2012

AGE

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For the evolution of life histories, the trade-off between survival and reproduction is fundamental. Because sexes optimize fitness in different ways, this trade-off is expected to be resolved differently by males and females. Consequently, the sexes are predicted to respond differently to changes in resource availability. In fruit flies, research on dietary restriction has focused largely on females maintained in the absence of males, thereby neglecting sexual interactions that affect reproductive behavior of both sexes under more natural conditions. Here, we tested for the interactive effects of diet (40, 60, 100, and 300 % of standard yeast concentrations) and social environment (separate-sex vs. mixed-sex groups) on male and female Drosophila melanogaster life histories. Additionally, we evaluated the essential amino acid methionine as an agent that can uncouple the survival-reproduction trade-off. We show sex differences in the effect of social environment on survival patterns, but not on reproductive fitness. In females, yeast had a positive effect on reproduction and a negative effect on survival. In males, yeast had a negative effect on reproduction and the effect on survival depended on the social environment. Methionine reduced survival, but had no effect on reproduction. Our findings highlight the need to include both sexes and to vary social environments in research programs aimed at lifespan extension and call for further evaluation of the fecundityrestoring effect of methionine.

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Section: Introductionmentioning

confidence: 98%

Interactive effects of sex, social environment, dietary restriction, and methionine on survival and reproduction in fruit flies

Zajitschek

Friberg

et al. 2012

AGE

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“…Multiple groups have taken advantage of the relative simplicity of the fly diet and the ability to quickly generate large numbers of genetically identical subjects in order to test the effects of diet on complex, additive phenotypes such as longevity. Historically, reduction of total caloric intake (calorie restriction) has been associated with increased longevity in flies Grandison et al, 2009) and other organisms (Bishop and Guarente, 2007;Mercken et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Dietary composition regulatesDrosophilamobility and cardiac physiology

Bazzell

Ginzberg

Healy

et al. 2012

Journal of Experimental Biology

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SUMMARYThe impact of dietary composition on exercise capacity is a subject of intense study in both humans and model organisms. Interactions between diet and genetics are a crucial component of optimized dietary design. However, the genetic factors governing exercise response are still not well understood. The recent development of invertebrate models for endurance exercise is likely to facilitate study designs examining the conserved interactions between diet, exercise and genetics. As a first step, we used the Drosophila model to describe the effects of varying dietary composition on several physiological indices, including fatigue tolerance and climbing speed, cardiac performance, lipid storage and autophagy. We found that flies of two divergent genetic backgrounds optimize endurance and cardiac performance on relatively balanced low calorie diets. When flies are provided with unbalanced diets, diets higher in sugar than in yeast facilitate greater endurance at the expense of cardiac performance. Importantly, we found that dietary composition has a profound effect on various physiological indices, whereas total caloric intake per se has very little predictive value for performance. We also found that the effects of diet on endurance are completely reversible within 48h if flies are switched to a different diet. Supplementary material available online at

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“…This also differs from most vertebrate studies raising the question of whether results even reflect common mechanisms (see Grandison et al 2009a). Indeed, some results of DR in Drosophila trace to water supply (Ja et al 2009).…”

Section: Discussionmentioning

confidence: 88%

Influence of two methods of dietary restriction on life history features and aging of the cricket Acheta domesticus

Lyn

Naikkhwah

Aksenov

et al. 2010

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Studying aging is constrained using vertebrates by their longevity, size, ethical restrictions, and expense. The key insect model, Drosophila melanogaster, is holometabolous. Larvae feed on yeast in moist media and adults sponge food. Most aging studies are restricted to adults. Another key model, the nematode Caenorhabditis elegans, feeds on bacteria in moist media. For either invertebrate refreshing test materials, preventing degradation and obtaining accurate dosing are difficult even with synthetic media. The cricket Acheta domesticus has a short lifespan (∼120 days at 30°C) and is omnivorous. Age-matched cohorts are easily obtained from eggs. The life cycle is hemimetabolous and nymphs eat the same foods as adults. Growth is easily monitored, gender can be differentiated before maturity, and maturation is indicated by wings and mature genitalia. Crickets can be reared in large numbers at low cost. Test materials can be mixed into food and ingestion rates or mass budgets easily assessed. Here, we validate the cricket as a model of aging by testing two fundamental methods of restricting food intake: time-restricted access to food and dietary dilution. Growth, maturation, survivorship, and longevity varied with treatments and genders. Intermittent feeding (which is ineffective in flies) significantly extended longevity of crickets. Dietary dilution also extended longevity via remarkable prolongation of the juvenile period.

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Effect of a Standardised Dietary Restriction Protocol on Multiple Laboratory Strains of Drosophila melanogaster

Cited by 98 publications

References 46 publications

Interactive effects of sex, social environment, dietary restriction, and methionine on survival and reproduction in fruit flies

Interactive effects of sex, social environment, dietary restriction, and methionine on survival and reproduction in fruit flies

Dietary composition regulatesDrosophilamobility and cardiac physiology

Influence of two methods of dietary restriction on life history features and aging of the cricket Acheta domesticus

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