Effect of a Probiotic Strain, Enterococcus faecium, on the Immune Responses of Olive Flounder (Paralichthys olivaceus)

Kim, Yu Ri; Kim, Eun Young; Choi, Sun Young; Hossain, Muhammad Tofazzal; Oh, Ryun Kyoung; Heo, Won; Lee, Jong Min; Cho, Young Chai; Kong, In Soo

doi:10.4014/jmb.1108.08047

Cited by 47 publications

(23 citation statements)

References 21 publications

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“…BGO supplementation was unable to incite significantly higher serum MPO and antiprotease activities in this study. Similar results were reported for MPO (Park et al., ) and antiprotease activities (Heo et al., ; Kim et al., ) in fish fed different types of probiotics. The mechanism behind the innate immunity response to BGO upon its oral administration to olive flounders remains unclear, but it may be that beneficial intestinal bacteria metabolize the BGO.…”

Section: Discussionsupporting

confidence: 86%

“…So far information found, the in vivo study on BGO, as a potential prebiotic on terrestrial or aquatic species, has not been reported yet. In sequence of our previous successful studies on the effects of different immunostimulatotry agent and probiotics on olive flounders (Heo, Kim, Kim, Bai, & Kong, ; Hong et al., ; Kim et al., ) and concerning best response (growth and immunity) in olive flounder supplemented with 0.1% feed additives (Lee et al., ; Won, Kim, & Park, ; Yoo et al., ), in this research, we aimed to inoculate 0.1% BGO as a feed supplement in this commercially valuable cultured fish. The objective of the present study was to identify BGO's prebiotic potential in olive flounder aquaculture and elucidating its effects on the innate immunological parameters as well as prevention ability against streptococcosis.…”

Section: Introductionmentioning

confidence: 99%

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Effect of β-glucooligosaccharides as a new prebiotic for dietary supplementation in olive flounder (Paralichthys olivaceus ) aquaculture

et al. 2018

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b-Glucooligosaccharides (BGO), produced from barley b-glucan, were used as a feed supplement (0.1%) for juvenile olive flounder (Paralichthys olivaceus) to identify and quantify its oral administration effects on innate immunomodulation and infectious disease protection. Juvenile flounders (14 AE 0.5 g) were divided into two groups fed either 0.1% BGO (treatment) or a standard diet (control) for 8 weeks. At the end of the experiment, investigation of the effects was carried out through systemic studies on growth performance, serum and mucus biochemical parameters, innate immunity, microvillus length, and relative pro-inflammatory cytokine gene expression. The results demonstrated that the BGO diet produced slightly higher levels of growth performance, serum protein, microvillus length and pro-inflammatory cytokine gene (tumour necrosis factor-a, interleukin [IL]-1b, and IL-6) expression without any significant differences (p > .05). All innate immunity parameters were up-regulated by BGO administration and, among these, respiratory burst, lysozyme and superoxide dismutase (SOD) were significantly different (p < .05). Fish in the both groups were challenged with Streptococcus iniae (1.35 9 10 8 CFU/ml), and BGO group was focused to confirm the promotion of innate immunity parameters. The results showed a significantly (p < .05) lower death rate compared with that of the control. Therefore, BGO could be used as a new prebiotic in future olive flounder aquaculture as well as to control streptococcosis. K E Y W O R D Sb-glucooligosaccharides, growth performance, innate immune response, olive flounder, pro-inflammatory cytokine

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Effect of β-glucooligosaccharides as a new prebiotic for dietary supplementation in olive flounder (Paralichthys olivaceus ) aquaculture

et al. 2018

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“…Lysozyme activity is an important innate humoral parameter of bactericidal activity; lysozyme acts as an opsonin that activates both the complement system and phagocytes (Burge, Madigan, Burnett, & Burnett, ; Dash, Das, Samal, & Thatoi, ; Magnadottir, ). Importantly, lysozyme not only destroys the β‐(1,4)‐linked N ‐acetylglucosamine and N ‐acetylmuramic acid components of gram‐negative bacteria but also destroys other types of threatening pathogens that are phagocytosed by macrophages and neutrophils (Kim et al, ; Satyanarayana, Kumar, Naidu, & Prasad, ). In fact, phagocytes, mainly neutrophils, are believed to be the main sources of plasma lysozyme (Cecchini, Terova, Caricato, & Saroglia, ), and increases in lysozyme activity have been associated with neutrophilia (Muona & Soivio, ).…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

et al. 2019

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This study investigated the effects of a synbiotic Lacto Forte on growth performance, haemato-immunological responses, plasma bactericidal capacity, histological profiles and resistance of Oreochromis niloticus to Pseudomonas fluorescens. The experimental fish were divided into three groups; two of them (T1 and T2) were given Lacto Forte incorporated diets at increasing level (0.7 and 1.5 g/kg, respectively) and an additive-free basal diet served as the control (T0) for 30 and 45 days. At the end of the feeding trial, a challenge was performed using a virulent strain of P. fluorescens and mortalities were recorded over an additional 14-days period. The results showed that Lacto Forte has a pronounced effect on haematological and growth performance parameters at 1.5 g/kg rather than 0.5 g/kg (p < 0.05). Plasma lysozyme, proteases, antiproteases and bactericidal capacity were significantly enhanced (p < 0.05) in Lacto Forte-treated groups, particularly after 45 days of supplementation. Long-term supplementation with Lacto Forte (1.5 g/kg) induced degenerative changes in the liver, spleen and intestine. Additionally, a significant increase (p < 0.05) in survival rates were found in Lacto Forte-treated groups compared to the control one 14 days post-challenge. As a result, Lacto Forte fortifies tilapia immune response and can be used as a surrogate for antibiotics to control P. fluorescens. K E Y W O R D S bactericidal, growth performance, Innate immunity, Oreochromis niloticus, Pseudomonas fluorescens, synbiotic Lacto Forte How to cite this article: Ismail M, Wahdan A, Yusuf MS, Metwally E, Mabrok M. Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease in Oreochromis niloticus. Aquac Res. 2019;50:2545-2562.

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“…The second most investigated genus is Lactococcus (Balcázar et al, 2007b ; Kim et al, 2013 ; Beck et al, 2015 , 2016 ; Nguyen et al, 2017 ). Genera of Enterococcus, Pediococcus , and Leuconostoc have also been studied at a significant level; Enterococcus (Wang et al, 2008 ; Kim et al, 2012 ; Rodriguez-Estrada et al, 2013 ; Matsuura et al, 2017 ), Pediococcus (Neissi et al, 2013 ; Dawood et al, 2016a ; Kaew-on et al, 2016 ), Leuconostoc (Balcázar et al, 2007b ). Although the majority of the studies used a specific strain of live LAB (Table 7 ), some studies were performed with their inactivated form of LAB (Table 8 ).…”

Section: Practical Uses Of Lab As An Immunostimulant In Finfish Aquacmentioning

confidence: 99%

“…As expected, a vast majority of studies employed dietary LAB as the mode of administration. However, some studies treated the fish by intraperitoneal injection (Kim et al, 2012 ; Matsuura et al, 2017 ) or immersion in a LAB-containing bath (Wang et al, 2008 ). Many studies indicated that the feeding administration demonstrated better immunostimulant effects, compared to any other modes of application.…”

Section: Practical Uses Of Lab As An Immunostimulant In Finfish Aquacmentioning

confidence: 99%

Lactic Acid Bacteria in Finfish—An Update

et al. 2018

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A complex and dynamic community of microorganisms, play important roles within the fish gastrointestinal (GI) tract. Of the bacteria colonizing the GI tract, are lactic acid bacteria (LAB) generally considered as favorable microorganism due to their abilities to stimulating host GI development, digestive function, mucosal tolerance, stimulating immune response, and improved disease resistance. In early finfish studies, were culture-dependent methods used to enumerate bacterial population levels within the GI tract. However, due to limitations by using culture methods, culture-independent techniques have been used during the last decade. These investigations have revealed the presence of Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Streptococcus, Carnobacterium, Weissella, and Pediococcus as indigenous species. Numerous strains of LAB isolated from finfish are able to produce antibacterial substances toward different potential fish pathogenic bacteria as well as human pathogens. LAB are revealed be the most promising bacterial genera as probiotic in aquaculture. During the decade numerous investigations are performed on evaluation of probiotic properties of different genus and species of LAB. Except limited contradictory reports, most of administered strains displayed beneficial effects on both, growth—and reproductive performance, immune responses and disease resistance of finfish. This eventually led to industrial scale up and introduction LAB-based commercial probiotics. Pathogenic LAB belonging to the genera Streptococcus, Enterococcus, Lactobacillus, Carnobacterium, and Lactococcus have been detected from ascites, kidney, liver, heart, and spleen of several finfish species. These pathogenic bacteria will be addressed in present review which includes their impacts on finfish aquaculture, possible routes for treatment. Finfish share many common structures and functions of the immune system with warm-blooded animals, although apparent differences exist. This similarity in the immune system may result in many shared LAB effects between finfish and land animals. LAB-fed fish show an increase in innate immune activities leading to disease resistances: neutrophil activity, lysozyme secretion, phagocytosis, and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α). However, some LAB strains preferentially induces IL-10 instead, a potent anti-inflammatory cytokine. These results indicate that LAB may vary in their immunological effects depending on the species and hosts. So far, the immunological studies using LAB have been focused on their effects on innate immunity. However, these studies need to be further extended by investigating their involvement in the modulation of adaptive immunity. The present review paper focuses on recent findings in the field of isolation and detection of LAB, their administration as probiotic in aquaculture and their interaction with fish immune responses. Furthermore, the mode of action of probiotics on finfish are discussed.

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Effect of a Probiotic Strain, Enterococcus faecium, on the Immune Responses of Olive Flounder (Paralichthys olivaceus)

Cited by 47 publications

References 21 publications

Effect of β-glucooligosaccharides as a new prebiotic for dietary supplementation in olive flounder (Paralichthys olivaceus ) aquaculture

Effect of β-glucooligosaccharides as a new prebiotic for dietary supplementation in olive flounder (Paralichthys olivaceus ) aquaculture

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

Lactic Acid Bacteria in Finfish—An Update

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