Effect of a New Variety of Apis mellifera Propolis onMutans Streptococci

Abstract

The effects of a new variety of propolis, from Northeastern Brazil (BA), on growth of mutans streptococci, cell adherence, and water-insoluble glucan (WIG) synthesis were evaluated. Propolis from Southeastern (MG) and Southern (RS) Brazil were also tested as an extension of our previous work. Ethanolic extracts of propolis (EEP) were prepared and analyzed by reversed-phase HPLC. For the antibacterial activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEPs against Streptococcus mutans, S. sobrinus, and S. cricetus were determined. Cell adherence of S. mutans and S. sobrinus to a glass surface was measured spectrophotometrically at 550 nm. WIG synthesized from sucrose by glucosyltransferase (Gtf) was extracted and quantified by the phenol-sulfuric method. The HPLC profile of the new variety of propolis was entirely different from Southeastern and Southern propolis. Neither flavonoid aglycones nor p-coumaric acid were detected in EEP BA. All EEPs demonstrated biological activities against mutans streptococci; EEP BA showed the highest potency in all in vitro parameters evaluated in this study. The ranges of MIC values were 50 (EEP BA)-400 microg/ml (MG), for S. mutans; and 25 (BA)-400 microg/ml (MG), for S. sobrinus and S. cricetus. The bactericidal concentration of EEPs was four to eight times the MIC values. The adherence of S. mutans and S. sobrinus cells and WIG synthesis were markedly inhibited by EEPs, demonstrating significant inhibition at all concentrations compared with the control (80% ethanol) (p<0.05). EEP BA showed 80% inhibition of cell adherence and WIG synthesis at concentrations as low as 12.5 and 7.8 microg/ml, respectively. The results show that the new variety of propolis was exceptionally effective in all in vitro parameters tested against mutans streptococci; biological effects of propolis are likely not to be due solely to flavonoids and (hydroxy)cinnamic acid derivatives.

Antioxidant Activity and Phenolic Composition of Herbal Infusions Consumed in Brazil Activid Ad Antioxidante Y Compuestos Fenólicos en Infusiones Herbarias Consumid as en Brasil

“…The infusions were analyzed according to Koo et al (2000) by injecting 20 µL of each extract in a liquid chromatograph connected to a photodiode-array detector at 260 nm (chosen wavelength after scanning from 200 nm to 600 nm, for it was the point of highest absorption of compounds) and a reversed-phase column Shimadzu ODS (250 x 4.6 mm) 5 µm particle size. Mobile phase was water (solvent A) and methanol (solvent B), at a flow rate of 1 mL/min.…”

Própolis do sudeste e nordeste do Brasil: influência da sazonalidade na atividade antibacteriana e composição fenólica

“…Estes diferentes tipos de própolis também apresentaram, conseqüentemente, propriedades biológicas distintas. Dentre eles, um proveniente da região Nordeste (tipo 6) e outro da região Sudeste (tipo 12) demonstraram atividade antimicrobiana sobre a bactéria S. mutans 6,7 . As própolis do tipo 6 apresentaram uma composição química distinta dos demais tipos, principalmente pela completa ausência de flavonóides e a presença de compostos de natureza mais apolar, entre eles ácidos graxos 22,23 .…”

“…É usada na medicina desde 300 a.C. tendo-se destacado tanto por suas propriedades biológicas, como as atividades antimicrobiana, antiinflamatória, cicatrizante, anestésica, antitripanossomal [1][2][3][4][5] , anticariogênica 6,7 , antiviral, anticarcinogênica e antioxidante [8][9][10][11] , quanto pela possibilidade de aplicação nas indústrias farmacêutica e alimentícia, como alimento funcional 12 .…”

Isolamento e identificação de compostos com atividade antibacteriana da própolis vermelha brasileira

“…Existem vários relatos atribuindo à própolis as mais variadas aplicações na medicina popular e, de fato, os estudos vêm corroborando que a própolis possui um grande potencial terapêutico, principalmente em relação às atividades antimicrobiana, antiinflamatória, cicatrizante, anestésica, antitripanossomal (GHISALBERTI, 1979;MAREKOV, 1989;KHAYYAL;ELGHAZALY;ELKHATIB, 1993;KUJUMGIEV et al, 1999;MARCUCCI et al, 2001;ALENCAR, 2002;MONTPIED et al, 2003, ALENCAR et al, 2007, anticariogênica (PARK et al 1998a;PARK et al, 1998b;KOO et al, 1999;KOO et al, 2000a;KOO et al, 2000b;ROSALEN et al, 2002;MURATA et al, 2003;DUARTE et al, 2003;HAYACIBARA et al, 2005;CASTRO et al, 2007), antiviral, anticarcinogênica, antioxidante (BURDOCK, 1998MARCUCCI, 1996;CHEN et al, 1996;BANKSOTA et al, 2000;ALENCAR, 2002;CHEN et al, 2003;NAGAI et al, 2003;ASO et al, 2004;ISHIKAWA et al, 2004;NAKAYAMA, 2004;OLDONI, 2007) e fitotóxica (GHISALBERTI, 1979;JOHNSON;EISCHEN;GIANASSI, 1994).…”

“…Para a determinação da Concentração Bactericida Mínima, segundo a técnica descrita por Koo et al (2000b) Para a determinação da CBM, uma alíquota de 10 µL do meio de cultura dos poços considerados inibitórios foram semeados em placas de Petri contendo BHI ágar e incubados a 37ºC por 24 horas. A CBM foi considerada como a menor concentração na qual não houve crescimento de colônias na superfície do meio de cultura.…”