Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells

Song, Seo-Hyeon; Kim, In‐Hye; Nam, Taek‐Jeong

doi:10.3892/ijmm.2012.899

Cited by 11 publications

(14 citation statements)

References 25 publications

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“…At low concentrations, CGF induced cell proliferation with a maximum increase in cell viabilities at E max of 0.001 g/L for all cell types. This is in good agreement with previous studies which have shown that extracts of C. vulgaris are cell proliferative at lower concentrations while being cytotoxic at high concentrations ( Yusof et al, 2010 ; Song et al, 2012 ). In addition, our study revealed that variable cytotoxicity was observed amongst mammalian cell lines, with HDF displaying the highest sensitivity with an average CC 50 value of 0.597 g/L (95% CI: 0.508–0.710) and ADSC displaying the lowest sensitivity at CC 50 value of 4.645 g/L (95% CI: 2.932–7.172) ( Supplementary Table S2 ).…”

Section: Resultssupporting

confidence: 93%

“…Table 1 shows the proximate crude composition of CGF. The soluble extract contained approximately 67.1% crude protein, 27.4% carbohydrate, and 5.7% crude ash, which is in good agreement with a previous study ( Song et al, 2012 ). In addition, the trace metals and microbial contents of CGF were found to be within the prescribed limits according to Singapore Medicines Act for human usage ( Koh and Woo, 2000 ).…”

Section: Resultssupporting

confidence: 92%

“…Given C. vulgaris ’ nutritious profile, some literatures have reported its potential use as an FBS alternative for in vitro cell culture. For example, Song et al (2012) demonstrated that their hot water C. vulgaris extract stimulated proliferation of rat intestinal epithelial (IEC-6) cells after 24 h of incubation at serum-free conditions, via activation of the mitogen-activated protein kinase (MAPK), phosphoinositide-3-kinase–protein kinase B (PI3K/Akt), and canonical wingless-related integration site (Wnt) pathways. This study iterated that water extracts of C. vulgaris contained bioactive polysaccharides, that exerted important cell proliferative effects through gene induction pathways.…”

Section: Introductionmentioning

confidence: 99%

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Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

Chua

Zhang³

et al. 2020

Front. Bioeng. Biotechnol.

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The global cell culture market is experiencing significant growth due to the rapid advancement in antibody-based and cell-based therapies. Both rely on the capacity of different living factories, namely prokaryotic and eukaryotic cells, plants or animals for reliable and mass production. The ability to improve production yield is of important concern. Among many strategies pursued, optimizing the complex nutritional requirements for cell growth and protein production has been frequently performed via culture media component titration and serum replacement. The addition of specific ingredients into culture media to modulate host cells' metabolism has also recently been explored. In this study, we examined the use of extracted bioactive components of the microalgae Chlorella vulgaris, termed chlorella growth factor (CGF), as a cell culture additive for serum replacement and protein expression induction. We first established a chemical fingerprint of CGF using ultraviolet-visible spectroscopy and liquid chromatography-mass spectrometry and evaluated its ability to enhance cell proliferation in mammalian host cells. CGF successfully promoted the growth of Chinese hamster ovary (CHO) and mesenchymal stem cells (MSC), in both 2D and 3D cell cultures under reduced serum conditions for up to 21 days. In addition, CGF preserved cell functions as evident by an increase in protein expression in CHO cells and the maintenance of stem cell phenotype in MSC. Taken together, our results suggest that CGF is a viable culture media additive and growth matrix component, with wide ranging applications in biotechnology and tissue engineering.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

Chua

Zhang³

et al. 2020

Front. Bioeng. Biotechnol.

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“…Cell proliferation is induced through intracellular signal transduction mediated by receptor tyrosine kinases (RTKs), such as epidermal growth factor receptor (EGFR) (8). RTKs are cell membrane receptors for growth factors and other extracellular ligands.…”

Section: Introductionmentioning

confidence: 99%

The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway

Lee

Kim

Choi

et al. 2015

International Journal of Molecular Medicine

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For a number of years, seaweed has been used as a functional food in Asian countries, particularly in Korea, Japan and China. Pyropia yezoensis is a marine red alga that has potentially beneficial biological activities. In this study, we examined the mechanisms through which a Pyropia yezoensis peptide [PYP1 (1–20)] induces the proliferation of IEC-6 cells, a rat intestinal epithelial cell line, and the involvement of the epidermal growth factor receptor (EGFR) signaling pathway. First, cell viability assay revealed that PYP1 (1–20) induced cell proliferation in a concentration-dependent manner. Subsequently, we examined the mechanisms responsible for this induction of proliferation induced by PYP1 (1–20). EGFR is widely expressed in mammalian epithelial tissues, and the binding of this ligand affects a variety of cell physiological parameters, such as cell growth and proliferation. PYP1 (1–20) increased the expression of EGFR, Shc, growth factor receptor-bound protein 2 (Grb2) and son of sevenless (SOS). EGFR also induced the activation of the Ras signaling pathway through Raf, MEK and extracellular signal-regulated kinase (ERK) phosphorylation. In addition, cell cycle analysis revealed the expression of cell cycle-related proteins. The results demonstrated an increased number of cells in the G1 phase and an enhanced cell proliferation. In addition, the upregulation of cyclin D, cyclin E, Cdk2, Cdk4 and Cdk6 was observed accompanied by a decreased in p21 and p27 expression. These findings suggest that PYP1 (1–20) stimulates the proliferation of rat IEC-6 cells by activating the EGFR signaling pathway. Therefore, PYP1 (1–20) may be a potential source for the development of bio-functional foods which promotes the proliferation of intestinal epithelial cells.

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“…3B ) expression levels of IGF-IR, IRS-1, Shc and PY-99. IGF-IR stimulates the proliferation of various cell types and inhibits apoptosis ( 25 ).…”

Section: Resultsmentioning

confidence: 99%

A peptide from Porphyra yezoensis stimulates the proliferation of IEC-6 cells by activating the insulin-like growth factor I receptor signaling pathway

Lee

Kim

Choi

et al. 2014

International Journal of Molecular Medicine

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Porphyra yezoensis (P. yezoensis) is the most noteworthy red alga and is mainly consumed in China, Japan and Korea. In the present study, the effects of a P. yezoensis peptide (PY-PE) on cell proliferation and the associated signaling pathways were examined in IEC-6 rat intestinal epithelial cells. First, the MTS assay showed that PY-PE induced cell proliferation in a dose-dependent manner. Subsequently, the mechanism behind the proliferative activity induced by PY-PE was determined. The insulin-like growth factor-I receptor (IGF-IR) signaling pathway was the main focus as it plays an important role in the regulation of cell growth and proliferation. PY-PE increased the protein and mRNA expression of IGF-IR, insulin receptor substrate-1, Shc and PY-99. In addition, PY-PE stimulated extracellular signal-regulated kinase phosphorylation and phosphatidylinositol 3-kinase/Akt activation but inhibited p38 and c-Jun N-terminal kinase phosphorylation. Furthermore, PY-PE treatment increased protein and mRNA expression levels of activator protein-1, which regulates cell proliferation and survival, in the nuclear fraction. These results have significant implications for understanding the role of cell proliferation signaling pathways in intestinal epithelial cells.

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Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells

Cited by 11 publications

References 25 publications

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway

A peptide from Porphyra yezoensis stimulates the proliferation of IEC-6 cells by activating the insulin-like growth factor I receptor signaling pathway

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