A peptide from Porphyra yezoensis stimulates the proliferation of IEC-6 cells by activating the insulin-like growth factor I receptor signaling pathway

Lee, Min‐Kyeong; Kim, In‐Hye; Choi, Youn‐Hee; Nam, Taek‐Jeong

doi:10.3892/ijmm.2014.2037

Cited by 20 publications

(19 citation statements)

References 40 publications

(34 reference statements)

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“…Food derived bioactive peptides have been shown to modulate intestinal epithelial cell differentiation and cytokine production [ 76 ]. Among these, a bioactive peptide derived from Porphyra yezoensis , termed PY-PE or PYP1, whose sequence is A-L-E-G-G-K-S-S-G-G-G-E-A-T-R-D-P-E-P-T, has been shown to induce the proliferation of IEC-6 cells, a rat intestinal epithelial cell line [ 77 , 78 ]. The stimulation of two different molecular signaling pathways has been found to be involved in this effect.…”

Section: Red Seaweeds and Intestinal Mucosal Barrier Functionmentioning

confidence: 99%

“…Peptide PY-PE also stimulates the expression of type I IGFR, which is involved in the promotion of cell proliferation and the inhibition of apoptosis [ 77 ], and of the adaptor molecules insulin receptor substrate-1 (IRS-1) and Shc. As a result PY-PE stimulates the phophatidylinositol 3-kinase (PI3K)/protein kinase B (PKB or AKT) pathway, ERK, and the expression of AP-1 proteins c-Jun and c-Fos [ 78 ]. Therefore PY-PE induces cell proliferation through the stimulation of different receptor and signal transduction pathways in IEC-6 cells [ 77 , 78 ].…”

Section: Red Seaweeds and Intestinal Mucosal Barrier Functionmentioning

confidence: 99%

“…As a result PY-PE stimulates the phophatidylinositol 3-kinase (PI3K)/protein kinase B (PKB or AKT) pathway, ERK, and the expression of AP-1 proteins c-Jun and c-Fos [ 78 ]. Therefore PY-PE induces cell proliferation through the stimulation of different receptor and signal transduction pathways in IEC-6 cells [ 77 , 78 ].…”

Section: Red Seaweeds and Intestinal Mucosal Barrier Functionmentioning

confidence: 99%

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Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota

et al. 2015

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Based on their composition, marine algae, and namely red seaweeds, are good potential functional foods. Intestinal mucosal barrier function refers to the capacity of the intestine to provide adequate containment of luminal microorganisms and molecules. Here, we will first outline the component of seaweeds and will summarize the effects of these on the regulation of mucosal barrier function. Special attention will be paid to unique components of red seaweeds: proteins and derived peptides (e.g., phycobiliproteins, glycoproteins that contain “cellulose binding domains”, phycolectins and the related mycosporine-like amino acids) together with polysaccharides (e.g., floridean starch and sulfated galactans, such as carrageenans, agarans and “dl-hybrid”) and minerals. These compounds have been shown to exert prebiotic effects, to regulate intestinal epithelial cell, macrophage and lymphocyte proliferation and differentiation and to modulate the immune response. Molecular mechanisms of action of peptides and polysaccharides are starting to be elucidated, and evidence indicating the involvement of epidermal growth factor receptor (EGFR), insulin-like growth factor receptor (IGFR), Toll-like receptors (TLR) and signal transduction pathways mediated by protein kinase B (PKB or AKT), nuclear factor-κB (NF-κB) and mitogen activated protein kinases (MAPK) will also be summarized. The need for further research is clear, but in vivo experiments point to an overall antiinflammatory effect of these algae, indicating that they can reinforce membrane barrier function.

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Section: Red Seaweeds and Intestinal Mucosal Barrier Functionmentioning

confidence: 99%

Section: Red Seaweeds and Intestinal Mucosal Barrier Functionmentioning

confidence: 99%

Section: Red Seaweeds and Intestinal Mucosal Barrier Functionmentioning

confidence: 99%

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Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota

et al. 2015

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“…Currently, only one study using a particular PYP peptide has reported its regulation of multiple cell growth-related signals in MCF-7 cells ( 25 ). PYP has also been shown to stimulate the proliferation of IEC-6 normal intestinal epithelial cells and is associated with the insulin-like growth factor I (IGF-IR) and epidermal growth factor receptor (EGFR) signaling pathways ( 26 , 27 ).…”

Section: Discussionmentioning

confidence: 99%

Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death

Choi

Kim

Mikami

et al. 2015

International Journal of Molecular Medicine

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In the present study, the chemoprotective effects of recombinant Pyropia yezoensis (P. yezoensis) protein 1 (PYP1) were examined in acetaminophen (APAP)-treated Chang liver cells. The analysis of P. yezoensis revealed the presence of both mature and immature variants of PYP1. PYP1s, designated as PYP1 (15 kDa), PYP1-AC (12 kDa) and PYP1-B (5 kDa), were successfully expressed in Escherichia coli, and their chemoprotective effects were then examined. In addition, a peptide of 11 residues (ALEGGKSSGGG), which is a common sequence at the N-terminus all of the PYP1s, was synthesized and examined. The effects of treatment with PYP1s and the synthetic peptide (SP) on cell proliferation were determined by MTS assay. Our results clearly demonstrated that treatment with all the PYP1s and SP significantly promoted the proliferation of Chang liver cells, protecting them against APAP. Thus, we concluded that recombinant PYP1s exert protective effects against injury to Chang liver cells.

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“…Previous studies by our group reported on the cellular and molecular effects of peptide and hydrophilic protein extracts of the brown seaweed Porphyra yezoensis (P. yezoensis) based on analyses of cytotoxicity (20,32), inflammation (33)(34)(35)(36)(37) and cell proliferation (38)(39)(40)(41). However, the biological effects of P. yezoensis protein (PYP) on cisplatin-induced renal injury have remained elusive.…”

Section: Protein Extracted From Porphyra Yezoensis Prevents Cisplatinmentioning

confidence: 99%

Protein extracted from Porphyra yezoensis prevents cisplatin-induced nephrotoxicity by downregulating the MAPK and NF-κB pathways

et al. 2017

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Acute renal failure is a serious complication of treatment with the anticancer drug cisplatin. Cisplatin exerts a cytotoxic effect on renal cells by inducing apoptosis through activating the tumor suppressor p53, nuclear factor‑κB (NF‑κB) and mitogen‑activated protein kinase (MAPK)/p38 pathways. Effects of protein extracts of the brown seaweed Porphyra yezoensis (P. yezoensis) on cytotoxicity, inflammation and cell proliferation have been reported; however, the effects of P. yezoensis protein (PYP) extract on cisplatin‑induced renal injury have remained elusive. The present study investigated the effects of PYP on cisplatin‑induced nephrotoxicity in the HK2 human proximal tubular epithelial cell line. PYP treatment reduced cisplatin‑induced apoptosis and death of HK2 cells by restoring the B‑cell lymphoma‑2 (Bcl‑2)‑associated X protein (Bax)/Bcl‑2 imbalance, cytochrome c release and caspase‑3 activation. In addition, PYP activated the redox‑sensitive transcription factor NF‑κB via stimulating the nuclear translocation of p65 in HK2 cells. PYP also restored renal antioxidant levels and increased the total and nuclear accumulation of NF erythroid 2‑related factor 2 in HK2 cells. PYP markedly attenuated cisplatin‑induced p38, MAPK and c‑Jun N‑terminal kinase phosphorylation. Furthermore, treatment with PYP ameliorated cisplatin‑induced renal cell damage by upregulating antioxidant defense mechanisms and downregulating the MAPK and NF‑κB signaling pathways. In addition, mice were divided into three treatment groups (control, cisplatin and PYP + cisplatin) and the effects of PYP were evaluated in a mouse model of cisplatin‑induced acute kidney injury. The concentrations of blood urea nitrogen and serum creatinine in the PYP + cisplatin group were lower than those in the cisplatin group. The mRNA expression levels of inflammatory factors interleukin‑6 (IL‑6), IL‑1β, tumor necrosis factor‑α and monocyte chemoattractant protein‑1 in the kidney tissues of the PYP + cisplatin group were also lower than those in the cisplatin group. These results suggest that PYP treatment had a preventive effect on nephrotoxicity, specifically by downregulating the MAPK and NF‑κB signaling pathways and the mRNA levels of inflammatory genes.

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A peptide from Porphyra yezoensis stimulates the proliferation of IEC-6 cells by activating the insulin-like growth factor I receptor signaling pathway

Cited by 20 publications

References 40 publications

Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota

Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota

Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death

Protein extracted from Porphyra yezoensis prevents cisplatin-induced nephrotoxicity by downregulating the MAPK and NF-κB pathways

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