Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death

Abstract

In the present study, the chemoprotective effects of recombinant Pyropia yezoensis (P. yezoensis) protein 1 (PYP1) were examined in acetaminophen (APAP)-treated Chang liver cells. The analysis of P. yezoensis revealed the presence of both mature and immature variants of PYP1. PYP1s, designated as PYP1 (15 kDa), PYP1-AC (12 kDa) and PYP1-B (5 kDa), were successfully expressed in Escherichia coli, and their chemoprotective effects were then examined. In addition, a peptide of 11 residues (ALEGGKSSGGG), which is a common sequence at the N-terminus all of the PYP1s, was synthesized and examined. The effects of treatment with PYP1s and the synthetic peptide (SP) on cell proliferation were determined by MTS assay. Our results clearly demonstrated that treatment with all the PYP1s and SP significantly promoted the proliferation of Chang liver cells, protecting them against APAP. Thus, we concluded that recombinant PYP1s exert protective effects against injury to Chang liver cells.

Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation

“…Many studies have explored the functional effects of peptides from P. yezoensis , which has exhibited enhanced cell viability by reducing ER stress induced via environmental pollutants [ 28 ]. It also had the ability to decrease oxidative stress caused by hydrogen peroxide and acetaminophen in Chang cells [ 21 , 23 , 24 ]. A recent study also demonstrated that a P. yezoensis peptide attenuated muscle atrophy in murine C2C12 myoblasts [ 26 ].…”

“…A red alga, Pyropia yezoensis ( P. yezoensis ), is widely cultured as food and as a nutritional supplement for its biofunctional components such as proteins, vitamins, minerals, and mycosporine-like amino acids [ 21 , 22 ]. Moreover, peptides or glycoproteins derived from P. yezoensis protect against oxidative stress induced by acetaminophen or hydrogen peroxide in Chang and human hepatoblastoma HepG2 cells [ 23 , 24 ], and promote cell proliferation in intestinal epithelial IEC-6 cells via the activation of phosphatidylinositol 3-kinase (PI3K)-Akt signaling [ 25 ]. A recent study also demonstrated that a peptide derived from P. yezoensis (PYP) regulated muscle atrophy by inhibiting atrogin1/muscle atrophy F-box (MAFbx) and muscle RING Finger 1 (MuRF1) signaling in mouse myoblast C2C12 cells [ 26 ].…”

Protective Effect of Pyropia yezoensis Peptide on Dexamethasone-Induced Myotube Atrophy in C2C12 Myotubes

“…P. yezoensis -derived peptides are known to exert various biological effects, including antioxidant [11], antitumor [12], and anti-inflammatory activities [13]. Previous studies have reported that peptides synthesized from P. yezoensis reduced the inflammatory stress induced by lipopolysaccharides in RAW 264.7 cells, and cytotoxicity induced by acetaminophen in Chang cells [13,14]. Moreover, P. yezoensis peptides prevented the endoplasmic reticulum stress induced by perfluorooctane sulfonate in Chang cells [15].…”

Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress

“…In particular, P. yezoensis peptide (PYP) is known to have antioxidant and chemoprotective properties (11,12). However, the bioactivity of PYP in ER stress conditions induced by environmental pollutants has yet to be elucidated.…”