Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion

Kazemzadeh, Shokoofeh; Rastegar, Tayebeh; Zangi, Bagher Minaei; Malekzadeh, Mehrnoush; Khanehzad, Maryam; Khanlari, Parastoo; Madadi, Shabnam; Bashghareh, Alieh; Hedayatpour, Azim

doi:10.1007/s43032-020-00447-5

Cited by 8 publications

(4 citation statements)

References 66 publications

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“…It is reported that paclitaxel or doxorubicin reduced the expression of 10 Andrologia proliferation-and meiosis-related proteins in germ cells, and this effect could be partially improved with Mel administration [26,44]. Moreover, adding Mel to the freezing medium can effectively promote the proliferation and differentiation of spermatogonial stem cells (SSCs) after transplantation in an azoospermia mouse model [45].…”

Section: Discussionmentioning

confidence: 99%

Melatonin Ameliorates Cyclophosphamide-Induced Spermatogenesis Disorder by Reducing Pyroptosis

et al. 2023

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As a chemotherapeutic drug, cyclophosphamide (CP) has a negative impact on male fertility due to its reproductive toxicity. Melatonin (Mel) promotes the male reproductive system and increases testosterone synthesis. This study is aimed at exploring the molecular mechanism of Mel as a protector of male fertility against CP-induced cytotoxicity. A CP toxicity model was established in adult ICR male mice by intraperitoneal injection of 100 mg/kg CP every other day for a week. Protective effects of Mel on the testis from CP-induced damage were evaluated using four groups of ICR male mice that received intraperitoneal injections of normal saline, 100 mg/kg CP, 10 mg/kg Mel, or the same dosage of CP and Mel, respectively. Testis morphology was observed by hematoxylin and eosin (HE) staining. Sperm quality parameters were evaluated, and sperm function was studied by in vitro fertilization (IVF). Proliferation, meiosis, and pyroptosis markers were examined by western blot. Results showed that CP treatment induced testis toxicity in a time-dependent manner with the most severe damage to the testis at two weeks post CP treatment. CP-treated mice showed reduced testicular weight and impaired spermatogenesis by downregulating PCNA and SYCP3, reduced serum testosterone levels, decreased sperm counts and motility, increased seminiferous tubule vacuolization, and oxidative damage to spermatogenic cells. All these effects, apart from testicular weight, could be ameliorated by Mel administration. The IVF experiment revealed that CP treatment reduced the rates of sperm fertilization and blastocyst development, which were also enhanced by Mel. Mel-treated mice also showed increased expression of proliferation-associated protein PCNA and meiosis-associated proteins REC8, STRA8, and SYCP3, which were all reduced by CP. Furthermore, Mel inhibited the pyroptosis of spermatogenic cells by reducing GSDMD and IL18 expression. In conclusion, this study indicated that Mel might protect the testis from CP-induced DNA damage to germ cells through the alleviation of pyroptosis.

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Section: Discussionmentioning

confidence: 99%

Melatonin Ameliorates Cyclophosphamide-Induced Spermatogenesis Disorder by Reducing Pyroptosis

et al. 2023

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“…Previous studies showed that normal function of SSCs decreases after the freeze-thaw process due to cryoinjuries. Therefore, it is necessary to add cryoprotectants to the freeze medium of SSCs [25,26]. In this study, melatonin as a cryoprotectant was added in the freeze medium to reduce injuries of cells after the freeze-thaw process.…”

Section: Discussionmentioning

confidence: 99%

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin

Farokhi

2021

OAJBS

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Cryopreservation of spermatogonial stem cells (SSCs) is an advantageous method to restore fertility in young boys' cancer survivors. Recently, for reduction of SSCs cryoinjering various antioxidants have been used. The purpose of this study was to evaluate antioxidant effects of melatonin in frozen-thawed SSCs. Spermatogonial stem cells were isolated from testes of neonate mice (3-6 days old) and their purities were measured by flow cytometry with GFRα1. After culturing, the cells were frozen in two groups 1control and 2-melatonin (100μM) for one month. Finally, the cell viability, colonization rate, intracellular ROS were evaluated after freezing thawing. Melatonin increased the viability, colonization of SSCs, and decreased intracellular ROS. The results of this study show that melatonin with antioxidant effects as a good option can be used for freezing and long-term storage of cells and infertility treatment in the clinic.

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“…The results showed that the presence of melatonin in the frozen base medium has an effective role in improving the quality of transplanting SSCs into adult male into mature azoospermic mice [ 28 ]. The same author conducted a similar research in 2021 and added melatonin to the basic freezing medium containing SSCs and checked the quality of these cells after transplanting into adult azoospermic male mice and reached a similar result [ 14 ].…”

Section: Reviewmentioning

confidence: 99%

“…This indicates the wide activity of this hormone produced by the pineal gland [ 2 , 11 , 12 ]. In many articles, melatonin has been added as a supplement to the spermatogonial stem cells (SSCs) culture system, and the role of this factor in proliferation and differentiation has been investigated [ 6 , 13 , 14 ]. In this article, we aim to compile articles that employ melatonin as a supplement in laboratory experiments, animal testing, or freezing solutions and explore the signaling role of this substance as an antioxidant and growth enhancer.…”

Section: Introductionmentioning

confidence: 99%

The effect of biological mechanisms of melatonin on the proliferation of spermatogonial stem cells: a systematic review

Navid,

Saadatian,

Talebi

et al. 2024

Anat Cell Biol

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In the last decade, melatonin has gained recognition as a potent scavenger and an effective antioxidant capable of neutralizing free radicals, including reactive oxygen species. Additionally, it exhibits anti-apoptotic properties. In this review, we will examine a compilation of articles that explore the cellular signaling function of melatonin on spermatogonial stem cells (SSCs) and adjacent cells such as Sertoli and Leydig cells. These cells play a crucial role in the proliferation of SSCs both in vitro and in vivo . In this review, we analyze the function of melatonin in the proliferation of SSCs from other aspects. For this purpose, we examine the articles based on the presence of melatonin on SSCs in four groups: As a supplement in SSCs medium culture, SSCs three-dimensional culture system, SSCs freezing medium, and as a therapeutic factor in vivo . Mechanisms of growth and proliferation of SSCs were considered. The purpose of this study is to investigate the potential effects of melatonin as a powerful antioxidant or growth stimulant for SSCs, both in vivo and in vitro .

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Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion

Cited by 8 publications

References 66 publications

Melatonin Ameliorates Cyclophosphamide-Induced Spermatogenesis Disorder by Reducing Pyroptosis

Melatonin Ameliorates Cyclophosphamide-Induced Spermatogenesis Disorder by Reducing Pyroptosis

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin

The effect of biological mechanisms of melatonin on the proliferation of spermatogonial stem cells: a systematic review

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