Effect of a Cultures of <I>Aspergillus oryzae </I>on Inflammatory Response and mRNA Expression in Intestinal Immune-Related Mediators of Male Broiler Chicks

Abstract

Effect of Aspergillus oryzae (CAO) culture on systemic inflammatory response and mRNA expression of immune-related mediators in intestine was studied in male broiler chicks. Feeding CAO alleviated enhancement of early inflammatory response such as changes in acute phase substance and growth performance during immunological stress induced by lipopolysaccharide and Sephadex. Furthermore, changes in mRNA expression of immune-related mediators in the upper and lower intestine at 5 and 14 days of age were investigated in chicks fed a diet with antibiotics, CAO or without both as control. The CAO and antibiotic feeding lowered interferon (IFN)-γ and interleukin (IL)-1β mRNA expression compared to the control group. The expressions of toll like receptor (TLR)-4 mRNA in the antibiotic and CAO fed group were greater than that of the control group at 5 days of age, but not at 14 days of age. The findings suggest that CAO has anti-inflammatory effect and changes in IFN-γ, IL-1β and TLR-4 mRNA in immune-related cells of the gut in the CAO fed chicks appears to be similar to that of the antibiotics fed group. These immune-related mediators in the intestine may be a useful marker to select alternative to antibiotics.

Probiotic effects of the fungi, Aspergillus niger on growth, immunity, haematology, intestine fungal load and digestive enzymes of the common carp, Cyprinus carpio

“…The immunomodulatory function of Aspergillus spp. has been also confirmed by many studies in other vertebrates (Abu Hafsa et al, 2016; El‐Deep et al, 2021; Saleh et al, 2017; Takahashi, 2012; Zamanizadeh et al, 2021).…”

“…Neutrophils are one of the first immune components to respond to inflammation (Ellis, 1999). In vertebrates (Abu Hafsa et al, 2016;El-Deep et al, 2021;Saleh et al, 2017;Takahashi, 2012;Zamanizadeh et al, 2021).…”

Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers

“…Pro-inflammatory cytokines such as IL-1β, IL-6 and tumor necrosis factor-α (TNF-α) originated from macrophages were the major cellular messengers initiating metabolic cascade alterations following the innate immune response ( Jiang et al., 2015 ). Lipopolysaccharide was demonstrated to increase pro-inflammatory cytokines secretion and suppress animal growth ( Takahashi, 2012 , Tan et al., 2014 , Li et al., 2015b ). Results of the present study results showed that serum IL-1β, IL-6 levels of broilers significantly increased after LPS injection, and then were gradually back to the previous level, which indicated that broilers began to adapt immunological stress and metabolic function gradually recovered to normal.…”

“…Nevertheless, the European Union and some other countries have forbidden the use of antibiotics in livestock feed according to consideration of antibiotics resistance causing negative effects on human health ( Huyghebaert et al., 2011 ). It is reported that regulation of immune system in birds by certain nutrients or antibiotic-free substances appears to be a way to avoid or lower use of antibiotics ( Takahashi, 2012 ). Therefore, development of safe and effective nutritional additives to modulate the immune function is of great significance to protect chickens from immune stress ( Liu et al., 2015 ).…”

Dietary methionine restriction alleviates oxidative stress and inflammatory responses in lipopolysaccharide-challenged broilers at early age

“…Lipopolysaccharide causes oxidative stress and promotes inflammatory reactions ( Rosadini and Kagan, 2017 ). It has been shown to increase the secretion of proinflammatory cytokines and inhibit animal growth ( Takahashi, 2012 ; Tan et al, 2014 ; Li et al, 2015 ). Our results revealed that LPS stimulation significantly increased serum inflammatory cytokines TNF-α, IL-1β, and IL-6, and reduced the concentration of anti-inflammatory cytokine IL-10 at both time intervals.…”