Effect of a Chlorhexidine-containing Adhesive on Dentin Bond Strength Stability

Abstract

A novel chlorhexidine-containing adhesive may offer comparable dentin-resin bond stability to the use of 2.0% chlorhexidine digluconate applied as a therapeutic primer while also providing a simplified clinical application technique. SUMMARYPurpose: The present study aimed to investigate a novel adhesive system containing 0.2% chlorhexidine digluconate (CHX) for its ability to improve the stability of the adhesive interface compared with the use of 2% CHX as a therapeutic primer. Furthermore, the study aimed to confirm the inhibitory properties of these CHX concentrations (0.2% and 2.0%) on dentin matrix metalloproteinase activity by gelatin zymography.Methods: Superficial dentin substrate for bonding was obtained from 120 non-carious human molars. A conventional adhesive Peak LC Bond and a CHX-containing adhesive Peak Universal Bond were used either in combination with 35% phosphoric acid (etch-and-rinse approach) or with self-etching primer (selfetch approach) for evaluation of the variables CHX treatment (2.0% therapeutic primer and 0.2% adhesive), adhesive approach (etch-andrinse and self-etch), and storage time (24 hours and six months). A bonding jig was used to fabricate composite cylinders, which were stored for either 24 hours or six months, after which shear bond strength (SBS) was evaluated using a notched-edge testing device. A three-way analysis of variance and a Student t-test with a significance level of p,0.05 were used to analyze the data. Extracts from concentrated demineralized human dentin powder were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and incubated in the presence of 0.2% and 2.0% CHX.Results: No significant effect of CHX treatment, adhesive approach, storage time variables, or their interactions on mean SBS was

Dentin Bonding: Can We Make it Last?

“…[70][71][72][73] Several in vitro [74][75][76][77][78][79][80][81][82][83] and in vivo [84][85][86][87] studies have demonstrated that chlorhexidine preserves resin-dentin bonds by eliminating or at least delaying collagen degradation in the hybrid layer ( Figure 3). …”

MMP Inhibitors on Dentin Stability

“…The studies that used MMP inhibitors other than CHX (Breschi et al, 2010b;Cova et al, 2011) or applied the solution for < 60 s Breschi et al, 2010a;Leitune et al, 2011;Manfro et al, 2012) and the studies that incorporated the MMP inhibitors within the composition of the adhesive system (Zhou et al, 2009;De Munck et al, 2010;Yiu et al, 2012;Sabatini, 2013;Zhou et al, 2013) were not included in the meta-analysis.…”

Mecanismo de ação da clorexidina sobre as enzimas metaloproteinases e sua repercussão clínica: um estudo de revisão

“…Além disso, as MMPs têm papel fundamental no desenvolvimento e remodelação dos tecidos orais. Há um consenso de que as MMPs da matriz da dentina são fundamentais para a dentinogênese 5,6,23 . Por outro viés, essas enzimas também são responsáveis pela degradação dos componentes extracelulares da dentina após uma variação de pH tecidual.…”

“…As MMPs encontradas na dentina são produzidas pelos odontoblastos durante a produção da matriz extracelular e, após a calcificação da dentina, essas enzimas permanecem em sua forma inativa 3,4 . Entretanto, podem ser reativadas durante o processo carioso, devido à acidez do meio, propiciando a degradação da rede de colágeno 1,2,[5][6][7][8][9][10][11][12] . Assim como o desenvolvimento do processo carioso, a atuação de condicionadores ácidos para o emprego de sistemas adesivos, seja nos sistemas de condicionamento ácido total ou auto condicionantes, também pode ativar as MMPs 3,4,[7][8][9] , possibilitando a degradação da matriz de colágeno.…”