Effect of a carbonated HAP/β-glucan composite bone substitute on healing of drilled bone voids in the proximal tibial metaphysis of rabbits

Borkowski, Leszek; Pawłowska, Marta; Radzki, Radosław P.; Bieńko, Marek; Polkowska, Izabela; Belcarz, Anna; Karpiński, Mirosław; Słowik, T; Matuszewski, Łukasz; Ślósarczyk, A.; Ginalska, Grażyna

doi:10.1016/j.msec.2015.04.009

Cited by 29 publications

(19 citation statements)

References 45 publications

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“…Prior to surgery, animals were premedicated with medetomidine (Domitor®, Orion Corp., Finland; dose 0.5 mg/kg) and anesthetized with ketamine (Bioketan®, Biowet, Poland; 0.5 mg/kg). Surgical procedure was described previously by Borkowski et al [ 34 , 35 ]. Briefly, a defect, 4 mm in diameter and 6 mm in depth, was drilled in the proximal tibial metaphysis and filled with CHAP-glucan composite material.…”

Section: Methodsmentioning

confidence: 99%

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

et al. 2017

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Regeneration of bone defects was promoted by a novel β-glucan/carbonate hydroxyapatite composite and characterized by Raman spectroscopy, microCT and electron microscopy. The elastic biomaterial with an apatite-forming ability was developed for bone tissue engineering and implanted into the critical-size defects of rabbits’ tibiae. The bone repair process was analyzed on non-decalcified bone/implant sections during a 6-month regeneration period. Using spectroscopic methods, we were able to determine the presence of amides, lipids and assign the areas of newly formed bone tissue. Raman spectroscopy was also used to assess the chemical changes in the composite before and after the implantation process. SEM analyses showed the mineralization degree in the defect area and that the gap size decreased significantly. Microscopic images revealed that the implant debris were interconnected to the poorly mineralized inner side of a new bone tissue. Our study demonstrated that the composite may serve as a biocompatible background for collagen ingrowth and exhibits the advantages of applying Raman spectroscopy, SEM and microCT in studying these samples.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-017-0518-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

et al. 2017

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“…Composite samples (4 mm in diameter and 6 mm in depth) were implanted (surgical procedure as described by Borkowski et al [25]) into bone defects in the tibial metaphysis of 24 New Zealand male white rabbits with approval of the II Local Ethics Committee on Animal Research University of Life Sciences in Lublin, Poland (agreement no. 16/2010).…”

Section: Methodsmentioning

confidence: 99%

The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging

et al. 2015

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The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering. The composite is an artificial bone material with an apatite-forming ability for the bone repair process. Rabbit bone samples were tested with an implanted bioactive material for a period of several months. Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity. Samples were also imaged in transmission using an infrared microscope combined with a focal plane array detector. CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis. The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples. It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process. The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.

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“…These studies showed the beneficial synergistic effects of polycan and calcium lactate gluconate formulations against osteoarthritis. Borkowski et al investigated the synergistic effects of carbonated hydroxyapatite (CHAP) granules and β-glucans as a filler for bone defects in New Zealand white rabbits [ 184 ]. Significant integration of implants with bone tissue, as well as no signs of graft rejection, were observed, and the biomaterial showed a stimulating effect on bone formation and mineralization.…”

Section: Bone Regeneration/bone Injury Healing Effects Of β-Glucanmentioning

confidence: 99%

Clinical and Physiological Perspectives of β-Glucans: The Past, Present, and Future

Bashir

Choi

2017

IJMS

179

107

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β-Glucans are a group of biologically-active fibers or polysaccharides from natural sources with proven medical significance. β-Glucans are known to have antitumor, anti-inflammatory, anti-obesity, anti-allergic, anti-osteoporotic, and immunomodulating activities. β-Glucans are natural bioactive compounds and can be taken orally, as a food supplement, or as part of a daily diet, and are considered safe to use. The medical significance and efficiency of β-glucans are confirmed in vitro, as well as using animal- and human-based clinical studies. However, systematic study on the clinical and physiological significance of β-glucans is scarce. In this review, we not only discuss the clinical and physiological importance of β-glucans, we also compare their biological activities through the existing in vitro and animal-based in vivo studies. This review provides extensive data on the clinical study of β-glucans.

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Effect of a carbonated HAP/β-glucan composite bone substitute on healing of drilled bone voids in the proximal tibial metaphysis of rabbits

Cited by 29 publications

References 45 publications

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging

Clinical and Physiological Perspectives of β-Glucans: The Past, Present, and Future

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