Effect of 5-Aminolevulinic Acid (ALA) on Leaf Diurnal Photosynthetic Characteristics and Antioxidant Activity in Pear (Pyrus Pyrifolia Nakai)

Shen, Ming; Zhang, Zhi Ping; Wang, Liang Ju

doi:10.5772/26738

Cited by 4 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of studies revealed that 5‐ALA application promoted leaf photosynthesis under normal (Hotta et al , Wang et al , Shen et al ) or stress conditions (Wang et al , Youssef and Awad , Sun et al , Gao et al ). The transgenic Arabidopsis (Zhang et al ) or strawberry (Lin et al ) which was transformed by YHem1 also possessed higher photosynthetic capacity.…”

Section: Discussionmentioning

confidence: 99%

“…The second is photochemical reaction activity of photosystem (PS). With a plant efficiency analyzer (PEA) or multiple PEAs, several studies have revealed that 5‐ALA promotes not only the activity of oxygen‐evolving complex at the donor side of PSII reaction centers, but also the electron transfer rate at the acceptor side of PSII reaction centers (Sun et al , , Wang et al , Shen et al , Xie et al ). However, why 5‐ALA can accelerate photosynthetic electron transfer is not clear.…”

Section: Discussionmentioning

confidence: 99%

“…The measurements of diurnal photosynthesis were carried out according to the method described by Shen et al () with a portable photosynthesis system CIRAS‐2 (PP Systems, Amesbury, MA, USA). For every 2 h from 7:00 am to 17:00 pm, the net photosynthetic rate (P n ) was measured under the PPFD from a built‐in light source equal to the natural light intensities with the ambient temperatures.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Study on salt tolerance with YHem1 transgenic canola (Brassica napus)

Sun

Feng

Cui

et al. 2014

Physiologia Plantarum

View full text Add to dashboard Cite

5-Aminolevulinic acid (5-ALA) has been suggested for improving plant salt tolerance via exogenous application. In this study, we used a transgenic canola (Brassica napus), which contained a constituted gene YHem1 and biosynthesized more 5-ALA, to study salt stress responses. In a long-term pot experiment, the transgenic plants produced higher yield under 200 mmol L(-1) NaCl treatment than the wild type (WT). In a short-term experiment, the YHem1 transformation accelerated endogenous 5-ALA metabolism, leading to more chlorophyll accumulation, higher diurnal photosynthetic rates and upregulated expression of the gene encoding Rubisco small subunit. Furthermore, the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, catalase and ascorbate peroxidase, were significantly higher in the transgenic plants than the WT, while the levels of O2 ·(-) and malondialdehyde were lower than the latter. Additionally, the Na(+) content was higher in the transgenic leaves than that in the WT under salinity, but K(+) and Cl(-) were significantly lower. The levels of N, P, Cu, and S in the transgenic plants were also significantly lower than those in the WT, but the Fe content was significantly improved. As the leaf Fe content was decreased by salinity, it was suggested that the stronger salt tolerance of the transgenic plants was related to the higher Fe acquisition. Lastly, YHem1 transformation improved the leaf proline content, but salinity decreased rather than increased it. The content of free amino acids and soluble sugars was similarly decreased as salinity increased, but it was higher in the transgenic plants than that in the WT.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Study on salt tolerance with YHem1 transgenic canola (Brassica napus)

Sun

Feng

Cui

et al. 2014

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…And, it is worth emphasizing that ALA improves plant photosynthesis efficiency not only under normal conditions ( Hotta et al, 1997 ), but also under various stresses, such as cold ( Hotta et al, 1998 ), salt ( Nishihara et al, 2003 ), low light ( Wang et al, 2004 ), water deficit ( Liu et al, 2011 ), heat ( Zhang et al, 2012 ), and heavy metal stresses ( Ali et al, 2013 ; Tian et al, 2014 ), suggesting its great application potential in agriculture and forestry. However, to date, the proposed mechanisms underlying ALA-promoted photosynthesis include only the following: (1) boosting light-harvesting capability by increasing chlorophyll content ( Youssef and Awad, 2008 ), (2) improving photosynthetic electron transport activity ( Wang et al, 2010 ), (3) promoting antioxidant activity ( Nishihara et al, 2003 ), and (4) increasing rubisco activity by up-regulating transcription of gene encoding Rubisco small unit ( Shen et al, 2011 ). Therefore, the mechanism how ALA regulates plant photosynthesis and growth is still in its infancy.…”

Section: Introductionmentioning

confidence: 99%

ALA Inhibits ABA-induced Stomatal Closure via Reducing H2O2 and Ca2+ Levels in Guard Cells

Liu

Chen

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

5-Aminolevulinic acid (ALA), a newly proved natural plant growth regulator, is well known to improve plant photosynthesis under both normal and stressful conditions. However, its underlying mechanism remains largely unknown. Stomatal closure is one of the major limiting factors for photosynthesis and abscisic acid (ABA) is the most important hormone in provoking stomatal closing. Here, we showed that ALA significantly inhibited ABA-induced stomatal closure using wild-type and ALA-overproducing transgenic Arabidopsis (YHem1). We found that ALA decreased ABA-induced H2O2 and cytosolic Ca2+ accumulation in guard cells with stomatal bioassay, laser-scanning confocal microscopy and pharmacological methods. The inhibitory effect of ALA on ABA-induced stomatal closure was similar to that of AsA (an important reducing substrate for H2O2 removal), CAT (a H2O2-scavenging enzyme), DPI (an inhibitor of the H2O2-generating NADPH oxidase), EGTA (a Ca-chelating agent), and AlCl3 (an inhibitor of calcium channel). Furthermore, ALA inhibited exogenous H2O2- or Ca2+-induced stomatal closure. Taken together, we conclude that ALA inhibits ABA-induced stomatal closure via reducing H2O2, probably by scavenging, and Ca2+ levels in guard cells. Moreover, the inhibitive effect of ALA on ABA-induced stomatal closure was further confirmed in the whole plant. Finally, we demonstrated that ALA inhibits stomatal closing, but significantly improves plant drought tolerance. Our results provide valuable information for the promotion of plant production and development of a sustainable low-carbon society.

show abstract

“…A similar response was found in potatoes when measurements were conducted at noon and afternoon [68], suggesting that ALA promoted the turnover of the core proteins of the PSII reaction center when plants were experiencing stressful conditions. In pears, ALA depressed the noon break of leaf photosynthesis when the environment was not suitable [229]. In Kentucky bluegrass, ALA upregulated the gene expression of many photosynthetic electron transfer chains, including the ferredoxin-NADP + reductase gene, resulting in weakening photoinhibition [191].…”

Section: Photosynthesis and Stomatal Regulation Under Stressful Condi...mentioning

confidence: 99%

Regulation of 5-Aminolevunilic Acid and Its Application in Agroforestry

Wang,

Zhang,

Zhong

et al. 2023

Forests

View full text Add to dashboard Cite

The review briefly introduces the natural occurrence, physicochemical properties, and biosynthesis of 5-aminolevuinic acid (ALA) and highlights a variety of applications in the planting industry and its possible mechanisms. It has been known that ALA can be used as biological pesticides, fungicides, and herbicides when the concentrations are higher than 838 mg L−1 (about 5 mmol L−1). When ALA concentrations are 100–300 mg L−1, it can be used to thin surplus flowers in the spring of orchards and promote fruit coloration before maturation. When the concentrations are lower than 100 mg L−1, especially not higher than 10 mg L−1, ALA can be used as a new plant growth regulator to promote seed germination, plant (including root and shoot) growth, enhance stress tolerance, increase crop yield, and improve product quality. In photosynthesis, ALA is involved in the regulation of the whole process. In stress tolerance, ALA induces plant preventive and protective systems through the NO/H2O2 signaling network. In secondary metabolism, ALA regulates many gene expressions encoding transcription factors or function proteins to promote anthocyanin and flavonol biosynthesis and accumulation. In general, ALA promotes plant health and robustness, reduces the use of chemical fertilizers and pesticides—which is conducive to improving the ecological environment, human production, and living conditions—and has a broad application prospect in agroforestry production. As a new plant growth regulator with multiple and powerful functions, the underlying regulatory mechanisms need more study.

show abstract

Effect of 5-Aminolevulinic Acid (ALA) on Leaf Diurnal Photosynthetic Characteristics and Antioxidant Activity in Pear (Pyrus Pyrifolia Nakai)

Cited by 4 publications

References 31 publications

Study on salt tolerance with YHem1 transgenic canola (Brassica napus)

Study on salt tolerance with YHem1 transgenic canola (Brassica napus)

ALA Inhibits ABA-induced Stomatal Closure via Reducing H2O2 and Ca2+ Levels in Guard Cells

Regulation of 5-Aminolevunilic Acid and Its Application in Agroforestry

Contact Info

Product

Resources

About