Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage

Abstract

In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 106 colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as Lactobacillus plantarum. The characteristics of chemical and fermentation compounds indicated that PLA and the two strains efficiently improved the quality of the alfalfa silage. It can be concluded that the use of the strains and PLA can significantly improve the quality of silage.

Effects of Phenyllactic Acid, Lactic Acid Bacteria, and Their Mixture on Fermentation Characteristics and Microbial Community Composition of Timothy Silage

“…PL has been applied in feed diets for improving animal performance. A study from Wu et al (2020) has proved that PL exhibited a positive effect on the CP preservation of alfalfa silage. This situation was just observed in PI-treated silage.…”

“…A similar result was from Jung et al (2019) who reported that PL level was not consistent with LA production in kimchi fermentation. Wu et al (2020) used PL as an additive at ensiling and found that PL could promote the production of LA, AA, and PA in alfalfa silage. In the present study, the use of PL at ensiling did not enhance the fermentation performance of timothy silage.…”

“…Phenyllactic acid (PL) is an important broad-spectrum antimicrobial compound that inhibits the growth of undesirable microbes (most bacteria and some fungi) through multifaceted actions, even under low-temperature conditions ( Rajanikar et al, 2021 ). PL is mainly derived from LAB genera such as Lactobacillus , Pediococcus , and Weissella ( Bustos et al, 2018 ; Lipinska-Zubrycka et al, 2020 ; Wu et al, 2020 ). PL has been used in various bio-based materials in agricultural, pharmaceutical, and chemistry fields ( Mu et al, 2009 ).…”

“…In fact, PL is now well accepted as an alternative to antibiotics in livestock feeds ( Nazareth et al, 2020 ). A study from Wu et al (2020) has shown that PL could prevent crude protein (CP) degradation of alfalfa silage. However, limited information is available on how PL regulates microbiota for better silage preservation.…”

Lactic Acid Bacteria as Biopreservation Against Spoilage Molds in Dairy Products – A Review

“…plantarum was around 0.05 mg/mL. The addition of 3-phenyllactic acid prevented protein degradation during the ensiling period and improved the quality of alfalfa silage (Wu et al, 2020).…”