Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)

Gómez-Lobato, María E.; Hasperué, Joaquín H.; Civello, Pedro Marcos; Chaves, Alicia Raquel; Martínez, Gustavo

doi:10.1016/j.scienta.2012.07.017

Cited by 59 publications

(35 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies showed that chlorophyll loss of broccoli florets is associated with ethylene production in the vegetable or ethylene accumulation during storage (Chen et al, 2008;Tian et al, 1995;Costa et al, 2005;Gómez-Lobato et al, 2012a). Inhibiting ethylene production (Henzi et al, 1999) or ethylene action (Ku and Wills, 1999;Fernández-León et al, 2013;Gómez-Lobato et al, 2012b) can delay yellowing of broccoli. Scavenging ethylene may be an alternative approach for extending broccoli postharvest life (Esturk et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Yellowing of broccoli is also increased as the 1-aminocyclopropane-1-carboxylic acid (ACC) level increases, which can lead to increase in ethylene formation (Pogson et al, 1995;Chen et al, 2008). In contrast, inhibiting ethylene production by transformation with an antisense ACC oxidase gene (Henzi et al, 1999), or inhibiting ethylene action with 1-methylcyclopropene (Ku and Wills, 1999;Gómez-Lobato et al, 2012b;Fernández-León et al, 2013) can effectively delay yellowing of the florets, maintain quality and substantially extend the storage life of the broccoli.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of a novel PdCl2–CuSO4–based ethylene scavenger supported by acidified activated carbon powder and its effects on quality and ethylene metabolism of broccoli during shelf-life

Cao

et al. 2015

Postharvest Biology and Technology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of a novel PdCl2–CuSO4–based ethylene scavenger supported by acidified activated carbon powder and its effects on quality and ethylene metabolism of broccoli during shelf-life

Cao

et al. 2015

Postharvest Biology and Technology

View full text Add to dashboard Cite

“…First-strand cDNA was synthesized using the PrimeScript RT reagent Kit (TAKARA BIO, Inc., Shiga, Japan). The relative transcription level of each gene was estimated in terms of threshold cycles using the 2 –ΔΔCT method (Livak and Schmittgen, 2001), and broccoli β-actin was employed as an internal control (Gómez-Lobato et al, 2012). qRT-PCR was carried out using SYBR Premix Ex TaqII (Tli RNaseH Plus; TAKARA BIO, Inc., Shiga, Japan) on an ABI Prism ® 7900HT (Applied Biosystems, Carlsbad, CA, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage (Brassica macrocarpa Guss.) in Response to Plasmodiophora brassicae during Different Infection Stages

et al. 2016

View full text Add to dashboard Cite

Clubroot, one of the most devastating diseases to the Brassicaceae family, is caused by the obligate biotrophic pathogen Plasmodiophora brassicae. However, studies of the molecular basis of disease resistance are still poor especially in quantitative resistance. In the present paper, two previously identified genotypes, a clubroot-resistant genotype (wild cabbage, B2013) and a clubroot-susceptible genotype (broccoli, 90196) were inoculated by P. brassicae for 0 (T0), 7 (T7), and 14 (T14) day after inoculation (DAI). Gene expression pattern analysis suggested that response changes in transcript level of two genotypes under P. brassicae infection were mainly activated at the primary stage (T7). Based on the results of DEGs functional enrichments from two infection stages, genes associated with cell wall biosynthesis, glucosinolate biosynthesis, and plant hormone signal transduction showed down-regulated at T14 compared to T7, indicating that defense responses to P. brassicae were induced earlier, and related pathways were repressed at T14. In addition, the genes related to NBS-LRR proteins, SA signal transduction, cell wall and phytoalexins biosynthesis, chitinase, Ca2+ signals and RBOH proteins were mainly up-regulated in B2013 by comparing those of 90196, indicating the pathways of response defense to clubroot were activated in the resistant genotype. This is the first report about comparative transcriptome analysis for broccoli and its wild relative during the different stages of P. brassicae infection and the results should be useful for molecular assisted screening and breeding of clubroot-resistant genotypes.

show abstract

“…As in the case of HT, the 1-MCP treatment reduces activity of enzymes involved in ethylene biosynthesis and in chlorophyll degradation (Tian et al 1997, Gong and Mattheis 2003, Gómez-Lobato et al 2012). …”

Section: Postharvest Treatmentsmentioning

confidence: 99%

Effect of time of day for harvest and postharvest treatments on the sugar metabolism of broccoli (Brassica oleracea var. italica)

Hasperué¹,

Lemoine²,

Chaves³

et al. 2014

AFSci

Self Cite

View full text Add to dashboard Cite

Loss of sugars contributes to accelerate postharvest senescence of broccoli. Several treatments have been developed to delay senescence, but in many cases their effects on sugar metabolism were not analyzed. We studied the effect of harvest at different times of day (08:00, 13:00 and 18:00 h) and of several postharvest treatments as heat treatment (HT), modified atmosphere (MA) and 1-methylcylcopropene (1-MCP) on sugar levels and activities of enzymes related to sucrose and starch degradation. Harvesting at the end of day delayed the loss of chlorophylls and caused the lowest decrement in sugars, although no differences in invertase, sucrose synthase and β-amylase activities were detected among samples. Treatments of MA and 1-MCP caused a lower loss of glucose and fructose, while HT caused a lower decrement of sucrose. Treated samples maintained higher levels of chlorophylls. The treatments reduced the activity of invertase and sucrose synthase and induced higher levels of β-amylase activity. Harvesting at the end of day and performing simultaneously a MA treatment could be a good combination to maintain the green color of the inflorescence and sugar levels during postharvest of broccoli.

show abstract

Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)

Cited by 59 publications

References 29 publications

Preparation of a novel PdCl2–CuSO4–based ethylene scavenger supported by acidified activated carbon powder and its effects on quality and ethylene metabolism of broccoli during shelf-life

Preparation of a novel PdCl2–CuSO4–based ethylene scavenger supported by acidified activated carbon powder and its effects on quality and ethylene metabolism of broccoli during shelf-life

Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage (Brassica macrocarpa Guss.) in Response to Plasmodiophora brassicae during Different Infection Stages

Effect of time of day for harvest and postharvest treatments on the sugar metabolism of broccoli (Brassica oleracea var. italica)

Contact Info

Product

Resources

About