Effect of 0.9% saline solution and phosphate buffer saline at different temperatures and incubation times on the morphology of goat preantral follicles

Santos, Regiane R.; Silva, José Roberto Viana; Costa, Sónia; Rodrigues, Ana Paula Ribeiro; Lôbo, Raimundo Nonato Braga; Figueiredo, José Ricardo de

doi:10.1590/s1413-95962002000500007

Cited by 16 publications

(22 citation statements)

References 23 publications

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“…The band at 1239 cm −1 is related to the P=O asymmetrical stretching of PO 2 phosphodiester groups from phosphorylated molecules, whereas the band at 1080 cm −1 arises from the P=O symmetrical stretching of PO 2 phosphodiester groups from phosphorylated molecules and glycogen [21, 23]. Other bands at 1154, 1055, and 1015 cm −1 respectively are related to C–O vibrations from glycogen and other carbohydrates [20]. Finally, a band around 991 cm −1 is mainly due to C–O stretch from RNA ribose chain [17, 23].…”

Section: Resultsmentioning

confidence: 99%

“…The instrument has a fixed spectral resolution of 4 cm −1 . Once mPSCs culture reached confluence, and DKCs fulfilled 10, 15 and 20 days in culture, the cells were trypsinized and centrifuged at 1200 rpm for 3 min, the supernatant was removed, and cells were washed 3 times with PBS, once Santos et al [20] have stated that cells are preserved for longer periods of time in PBS, getting concentrated samples of ~10 5 cells in 3 μl which were placed on the surface of the ATR crystal and dried at room temperature for about 15 min to eliminate water excess measuring the spectra until absorption bands related to liquid water were undetectable. The infrared radiation propagated along the crystal to obtain the corresponding spectrum, which was the average of 120 data acquisitions.…”

Section: Methodsmentioning

confidence: 99%

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Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

et al. 2017

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BackgroundKidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.ResultsIn this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.ConclusionOur results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

et al. 2017

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“…These results demonstrate that medium composition is a very important concern for P. tajacu PFs storage. In fact, studies have shown that PFs use a different source of nutrients such as their own endogenous resource and also the contents of the medium used for preservation (Santos et al, 2002). It is known that with increase in time and temperature, depletion of the endogen reserve occurs, and the medium composition becomes the main source of nutrients (Celestino et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The phosphate buffered saline solution (PBS) has been commonly used for short-term ovarian tissue preservation in mammals (Andrade et al, 2002;Santos et al, 2002). However, as PBS is a very simple medium comprised of a few constituents, the use of richer substances is suggested for female genetic material preservation.…”

Section: Introductionmentioning

confidence: 99%

Short-term preservation of Pecari tajacu ovarian preantral follicles using phosphate buffered saline (PBS) or powdered coconut water (ACP(r)) media

Lima

Santos

Lima

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan blue test) and the other fragments were stored as previously described, until 24h and then analyzed. After 4h storage in ACP(r) medium, the follicular integrity was similar to control (87.8% vs 94.4%, respectively); however, ultrastructural analyses revealed swollen mitochondria as the first signals of PF degeneration. It was observed that ACP(r) (66.7%) was more efficient than PBS (49.4%) to preserve the morphological integrity after 36h storage (P<0.05); however, no differences were observed on follicular viability (P>0.05). In conclusion, the use of the ACP(r) is recommended for the short-term preservation of Pecari tajacupreantral follicles.

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“…Several media have been tested, including 0.9% saline solution (SS) (Munhoz and Luna ), PBS (Santos et al. ) and coconut water (Costa et al. ).…”

Section: Introductionmentioning

confidence: 99%

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

Leonel

Bento-Silva

Ambrozio

et al. 2013

Reprod Domestic Animals

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The aim of this study was to test the use of mechanical and mechanical-enzymatic methods, saline solution (SS), and PBS solution for the manipulation and isolation of mare ovarian preantral follicles (PAFs). The ovaries were subjected to mechanical isolation (mixer) alone or in association with enzymatic digestion (collagenase). Incubation times of 10 and 20 min were employed. In the first group, 4.1 ± 4.9 PAFs were harvested with the mechanical-enzymatic method vs 71.1 ± 19.2 with the mechanical procedure, showing a significant difference between methods; using SS and PBS, these numbers were 35.7 ± 34.3 and 39.6 ± 39.6, respectively, with no significant difference between solutions. In the second group, there was significant difference between methods, with 7.1 ± 10.6 follicles harvested with the mechanical-enzymatic method vs 63.2 ± 22.9 with the mechanical procedure; using SS and PBS, means were 35.5 ± 36.4 and 34.9 ± 31.1, respectively. The mechanical method proved more effective than the mechanical-enzymatic approach. Both SS and PBS can be used as a media for equine PAFs preparation.

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Effect of 0.9% saline solution and phosphate buffer saline at different temperatures and incubation times on the morphology of goat preantral follicles

Cited by 16 publications

References 23 publications

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

Short-term preservation of Pecari tajacu ovarian preantral follicles using phosphate buffered saline (PBS) or powdered coconut water (ACP(r)) media

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

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