2014
DOI: 10.1590/1678-7297
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Short-term preservation of Pecari tajacu ovarian preantral follicles using phosphate buffered saline (PBS) or powdered coconut water (ACP(r)) media

Abstract: We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed… Show more

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Cited by 9 publications
(13 citation statements)
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References 21 publications
(18 reference statements)
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“…DNA fragmentation (using TUNEL, ISEL or other techniques) was performed in ten studies (Jewgenow et al 1997, Wongsrikeao et al 2005, Goncalves et al 2015, Kamoshita et al 2016, Cavalcante et al 2017, Gastal et al 2017, Barberino et al 2018, Piras et al 2018, Vilela et al 2020. Four studies used embryo analyses (Wongsrikeao et al 2005, Naoi et al 2007, Ribeiro et al 2008, Wang et al 2011 and five assessed isolated follicles (Lucci et al 2007, Lopes et al 2009, Lima et al 2014, Correia et al 2017, Raffel et al 2020. One study considered offspring after IVF (Kamoshita et al 2016).…”
Section: Analyses Performedmentioning
confidence: 99%
“…DNA fragmentation (using TUNEL, ISEL or other techniques) was performed in ten studies (Jewgenow et al 1997, Wongsrikeao et al 2005, Goncalves et al 2015, Kamoshita et al 2016, Cavalcante et al 2017, Gastal et al 2017, Barberino et al 2018, Piras et al 2018, Vilela et al 2020. Four studies used embryo analyses (Wongsrikeao et al 2005, Naoi et al 2007, Ribeiro et al 2008, Wang et al 2011 and five assessed isolated follicles (Lucci et al 2007, Lopes et al 2009, Lima et al 2014, Correia et al 2017, Raffel et al 2020. One study considered offspring after IVF (Kamoshita et al 2016).…”
Section: Analyses Performedmentioning
confidence: 99%
“…Hal ini menandakan bahwa kualitas oosit yang dikoleksi dari ovarium yang berasal dari RPH perlu diperhatikan dengan baik, dalam rangka mendukung proses kompleks yang akan dilalui oleh oosit hingga dapat mencapai pematangan baik inti maupun sitoplasma yang dibutuhkan untuk mencapai tahap fertilisasi hingga perkembangan fetus (Krisher et al 2007). Untuk menjaga kualitas oosit agar tetap baik setelah proses pemotongan hewan menuju laboratorium dilakukan dengan cara menempatkan ovarium pada kondisi lingkungan yang tepat (Lima et al 2014). Waktu, suhu dan medium yang digunakan merupakan beberapa faktor yang berpengaruh terhadap kondisi ovarium selama penyimpanan.…”
Section: Kondisi Ovarium Pasca-pemotongan Ternakunclassified
“…In collared peccaries, the refrigeration of ovaries for up to 36 hours allowed supporting the morphological integrity and viability of PFs. Further, it was demonstrated that powdered coconut water media (66.7%) was more effective than the phosphate buffered saline (PBS, 49.4%) to preserve the morphological integrity after 36 hours storage, although without statistical differences on respect to the follicular viability [102]. Moreover, the solid surface vitrification of ovarian tissue using EG, DMSO, or dimethylformamide (DMF), at 3.0 or 6.0 M, preserved the morphological integrity of more than 70% PFs using any of these cryoprotectants [103].…”
Section: Female Gametes Cryopreservationmentioning
confidence: 99%