Effect-directed profiling of aqueous, fermented plant preparations via high-performance thin-layer chromatography combined with <i>in situ</i> assays and high-resolution mass spectrometry

Jamshidi-Aidji, Maryam; Macho, Jennifer; Mueller, Margit B.; Morlock, Gertrud E.

doi:10.1080/10826076.2019.1585631

Cited by 17 publications

(7 citation statements)

References 28 publications

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“…Inhibition of α-glucosidase was performed using the method described by Jamshidi-Aidji et al [ 23 ]. The TLC plate was developed, dried, and sprayed with substrate solution (60 mg 2-naphthyl- α -D-glucopyranoside in 50 mL of ethanol) and allowed to dry.…”

Section: Methodsmentioning

confidence: 99%

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Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

et al. 2022

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The effect-directed detection (EDD) of Schisandra rubriflora fruit and leaves extracts was performed to assess their pharmacological properties. The EDD comprised TLC—direct bioautography against Bacillus subtilis, a DPPH assay, as well as α-glucosidase, lipase, tyrosinase, and acetylcholinesterase (AChE) inhibition assays. The leaf extracts showed stronger antioxidant activity than the fruit extract as well as inhibition of tyrosinase and lipase. The fruit extract was found to be extremely active against B. subtilis and to inhibit α-glucosidase and AChE slightly more than the leaf extracts. UHPLC–MS/MS analysis was carried out for the bioactive fractions and pointed to the possible anti-dementia properties of the dibenzocyclooctadiene lignans found in the upper TLC fractions. Gomisin N (518 mg/100 g DW), schisanhenol (454 mg/100 g DW), gomisin G (197 mg/100 g DW), schisandrin A (167 mg/100 g DW), and gomisin O (150 mg/100 g DW) were the quantitatively dominant compounds in the fruit extract. In total, twenty-one lignans were found in the bioactive fractions.

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Section: Methodsmentioning

confidence: 99%

“…α-Glucosidase inhibitors are responsible for lowering blood glucose levels. Drugs based on these compounds are used to fight diabetes [ 23 ]. The next is the lipase activity assay.…”

Section: Introductionmentioning

confidence: 99%

Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

et al. 2022

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“…For the calibration curve, 1 to 15 µl/band were applied as track pattern using the Free mode option of the ATS4 (CAMAG). The HPTLC‐DPPH · , HPTLC‐AChE, and HPTLC‐α‐glucosidase assays were performed according to the methods of Jamshidi‐Aidji, Macho, Mueller, and Morlock (), Azadniya and Morlock () and Jamshidi‐Aidji and Morlock (). The HPTLC‐AChE assay was recently adjusted to the piezoelectric spraying using the Derivatizer (CAMAG) by Azadniya and Morlock ().…”

Section: Methodsmentioning

confidence: 99%

Isolation of flavonoids from Musa acuminata Colla (Simili radjah, ABB) and the in vitro inhibitory effects of its leaf and fruit fractions on free radicals, acetylcholinesterase, 15‐lipoxygenase, and carbohydrate hydrolyzing enzymes

et al. 2020

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Musa species are used traditionally for the management of many diseases. The study evaluated and compared anticholinesterase, anti‐inflammatory, antioxidant, and antidiabetic activities of Musa acuminata (Simili radjah, ABB) fruits and leaves fractions and characterized the bioactive compounds using HPTLC‐HRMS and NMR. Leaf fractions gave the higher biological activities than the fruit. Ethyl acetate fraction of the leaf had the highest total phenolic content (911.9 ± 1.7 mg GAE/g) and highest 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH·) scavenging activity (IC50, 9.0 ± 0.4 µg/ml). It also gave the most effective inhibition of acetylcholinesterase (IC50, 404.4 ± 8.0 µg/ml) and α‐glucosidase (IC50, 4.9 ± 1.6 µg/ml), but a moderate α‐amylase inhibition (IC50, 444.3 ± 4.0 µg/ml). The anti‐inflammatory activity of n‐butanol (IC50, 34.1 ± 2.6 µg/ml) and ethyl acetate fractions (IC50, 43.1 ± 11.3 µg/ml) of the leaf were higher than the positive control, quercetin (IC50, 54.8 ± 17.1 µg/ml). Kaempferol‐3‐O‐rutinoside and quercetin‐3‐O‐rutinoside (rutin) were identified as the bioactive compounds with antioxidant and antidiabetic activities from the ethyl acetate fraction of M. acuminata leaf. Practical applications All parts of Musa acuminata are known to be useful ethnomedicinally even as food. The leaves are mostly used to serve food and used for wrapping purposes. However, this study concluded that M. acuminata leaf is rich in bioactive flavonoids such as kaempferol‐3‐O‐rutinoside and rutin, with relatively high antioxidative, antidiabetic, and anti‐inflammatory activities. Therefore, aside the fact that the leaves can serve as potential drug leads for pharmaceutical industries, it can also be embraced in the food sector to produce supplements and/or nutraceuticals in the management of Alzheimer's, diabetes and other inflammatory diseases.

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“…Results were documented at VIS light. α-Glucosidase inhibitors are visible as white bands on the purple background [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Qualitative and Quantitative Evaluation of Rosavin, Salidroside, and p-Tyrosol in Artic Root Products via TLC-Screening, HPLC-DAD, and NMR Spectroscopy

et al. 2022

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Artic root is a well-known plant adaptogen with multipotential pharmacological properties. Thin-layer chromatography (TLC)—screening followed by diode-array high-performance liquid chromatography and nuclear magnetic resonance spectroscopy proved to be a reliable and convenient method for the simultaneous determination of the quality of various herbal raw materials and supplements. This combination allowed for comparing and differentiating arctic root samples as well as defining their authenticity. The study provided information on the chemical and biological properties of the seven chosen samples as well as qualitative and quantitative evaluation of the quality markers: rosavin, salidroside, and p-tyrosol. The absence of rosavin, salidroside, and p-tyrosol in three samples was detected using TLC screening and confirmed by HPLC-DAD and NMR. The paper highlighted the importance of quality control and strict regulation for herbal medicine supplements and preparations.

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Effect-directed profiling of aqueous, fermented plant preparations via high-performance thin-layer chromatography combined with in situ assays and high-resolution mass spectrometry

Cited by 17 publications

References 28 publications

Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis

Isolation of flavonoids from Musa acuminata Colla (Simili radjah, ABB) and the in vitro inhibitory effects of its leaf and fruit fractions on free radicals, acetylcholinesterase, 15‐lipoxygenase, and carbohydrate hydrolyzing enzymes

Qualitative and Quantitative Evaluation of Rosavin, Salidroside, and p-Tyrosol in Artic Root Products via TLC-Screening, HPLC-DAD, and NMR Spectroscopy

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