Effect and characterization of sodium lignosulfonate on alkali pretreatment for enhancing enzymatic saccharification of corn stover

Xu, Huanfei; Yu, Guang; Mu, Xindong; Zhang, Chunyan; DeRoussel, Paul; Liu, Chao; Li, Bin; Wang, Haisong

doi:10.1016/j.indcrop.2015.07.057

Cited by 71 publications

(69 citation statements)

References 38 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…48 Xu et al pre-treated corn stover with alkali, and the crystalline index increased from 0.43 to 0.51. 49 These results indicate that the effects of Fe 3+ /H 2 O 2 /citric acid pretreatment for both lignin removal and decreasing the crystalline zones of cellulose were comparable or even greater than that of the thermochemical pre-treatments used in other research (Fig. 6).…”

Section: Structural Features Of Fenton-pretreated Rice Strawsupporting

confidence: 49%

Fenton pre-treatment of rice straw with citric acid as an iron chelate reagent for enhancing saccharification

et al. 2017

View full text Add to dashboard Cite

The Fenton reagent has recently been verified as being effective for lignocellulose pretreatment. However, the Fenton reaction can only work under acidic conditions, leading to severe environmental pollution and increasing the cost for sewage treatment. This has made identification of iron chelates that could enhance Fenton degradation under neutral conditions important. In this study, citric acid, a non-toxic polyhydroxy carboxylic acid, was introduced as an iron chelate in Fenton pre-treatment of rice straw. Results demonstrated that citric acid has the ability to (i) maintain the oxidation capacity of the Fenton reagent under near-neutral conditions, (ii) enhance the degradation of lignin and reduce the crystallinity of rice straw from 0.41 to 0.21, (iii) promote the saccharification of rice straw (the maximum saccharification ratio of 35.7% was two-fold higher than that for the untreated rice straw), and (iv) elevate the maximum activity of b-glucosidase from 0.33 IU mL À1 to 0.61 IU mL À1 . Moreover, the effect of operational parameters such as pH and processing time on the saccharification of rice straw by Fenton pretreatment with and without citric acid has been demonstrated. The findings strongly support Fenton plus citric acid as an efficient and environmentally friendly lignocellulose pretreatment method.

show abstract

Section: Structural Features Of Fenton-pretreated Rice Strawsupporting

confidence: 49%

Fenton pre-treatment of rice straw with citric acid as an iron chelate reagent for enhancing saccharification

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Sulfur dioxide free steam pretreated hybrid poplar achieved a 32% improvement in hydrolysis efficiency after 30 min of low consistency valley beater refining, comparable to the high severity steam plus sulfur dioxide treatments (Dou et al 2016). Used in a sodium lignosulfonate biorefinery concept, PFI milling (Papirindustriens Forskningsinstitut, PFI -the Norwegian Paper and Fibre Research Institute), and disk refining were both shown to boost the total sugar yields of modified alkaline pretreated corn stover, from 72% to 79% (Xu et al 2015). Although increased sulfonation charges improved enzymatic hydrolysis, the refining improvement compared with the unrefined pretreated biomass was constant, relating to a constant pretreatment severity of 11 wt% sodium hydroxide cooked for 40 min at 120 °C.…”

Section: Benefits Of Mechanical Refining In the Biorefinery Concept Imentioning

confidence: 88%

Optimization of Pilot Scale Mechanical Disk Refining for Improvements in Enzymatic Digestibility of Pretreated Hardwood Lignocellulosics

Jones¹,

Venditti²,

Park³

et al. 2017

BioResources

View full text Add to dashboard Cite

Mechanical refining has potential application for overcoming lignocellulosic biomass recalcitrance to enzyme hydrolysis and improving biomass digestibility. This study highlighted the ability for a pilot scale disc refiner to improve the total carbohydrate conversion to sugars from 39% (unrefined hardwood sodium carbonate biomass) to 90% (0.13 mm gap, 20% consistency, ambient temperature) by optimizing the refining variables. The different biomass properties that changed with refining indicated the expected increase in sugar conversion. Controlling the refining parameters to narrower gaps and higher consistencies increased the resulting refined biomass hydrolysis. Positive correlations that increases in net specific energy (NSE) input and refining intensity (SEL) improved the enzymatic hydrolysis. In some severe cases, over-refining occurred when smaller gaps, higher consistencies, and more energy input reached a point of diminished return. The energy input in these scenarios, however, was much greater than realistically feasible for industrial application. Although well-established in the pulp and paper industry, gaps in understanding the fundamentals of refining remain. The observations and results herein provide the justification and opportunity for further mechanical refining optimization to maximize and adapt the mechanical refining technology for maximum efficiency within the process of biochemical conversion to sugar. Keywords: Hardwood biomass; Biochemical conversion; Pretreatment; Mechanical refining; Enzymatic hydrolysisContact information: Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005 USA; *Corresponding author: richard_venditti@ncsu.edu INTRODUCTION Biorefinery ConceptThe biorefinery concept, defined as "the sustainable processing of biomass into a spectrum of marketable products and energy," is an approach that can mitigate the negative environmental impacts of fuel and chemical production by providing alternatives to oil refining products (Cherubini 2010).Lignocellulosic biomass is an extremely diverse class of material and has a complex structure composed of cellulose, hemicellulose, and lignin naturally constructed in a multifaceted matrix. Understanding the chemical and biochemical challenges based on lignocellulosic biomass recalcitrance, which can be defined as the natural resistance to biological deconstruction, remains one of the major technical barriers to the commercialization of second generation cellulosic biorefineries (Zhao et al. 2012). Embracing the challenge as an intentional practice of engineered biomass deconstruction PEER-REVIEWED ARTICLE bioresources.com Jones et al. (2017). "Mechanical disk refining," BioResources 12(3), 4567-4593. 4568 allows for an opportunity to harvest the inherently useful properties of biomass for creating value-added products through the biorefinery concept. Many innovative technologies have been developed to address this issue and improve the biomass hydrolysis yield. The issue with these technologies...

show abstract

“…Essas celulases podem ser comerciais, purificadas ou presentes em extratos enzimáticos XU et al, 2015;ALRUMMAN, 2016).…”

Section: Sacarificação De Materiais Lignocelulósicos Usando Celulasesunclassified

“…É interessante observar que, para obter melhores resultados, alguns trabalhos reportam a suplementação de celulases comerciais com -glucosidases comerciais (XU et al, 2015;MUND et al, 2016). Essa suplementação foi realizada para evitar que a celobiose seja um dos principais produtos obtidos da hidrólise, uma vez que a função da -glucosidase é a produção de glicose a partir de celobiose (YOON et al, 2014 obtendo-se melhores resultados de hidrólise de bagaço de cana-de-açúcar em comparação ao uso dos extratos em separado .…”

Section: Sacarificação De Materiais Lignocelulósicos Usando Celulasesunclassified

“…realizaram o pré-tratamento com o bagaço de cana-de-açúcar usando NaOH 0,5 M por 2 h a 80 °C obtendo, no final da reação um aumento do teor de celulose e hemicelulose de 38,5 % e 20,6% para 63,4% e 26,1%, respectivamente e uma diminuição do teor de lignina de 25,0% para 8,3% (LIU et al, 2015). Maryana et al (2014) também obtiveram percentuais semelhantes usando NaOH 1N por 15 min a 100 °C (66,2% de celulose, 17,1% de hemicelulose e 8,3% de lignina), cuja única diferença dos resultados obtidos neste trabalho foi que houve redução do teor de hemicelulose de 22,9% para 17,1% (MARYANA et al, 2014).…”

Section: Pré-tratamento Alcalino Do Bagaço De Cana-de-açúcarunclassified

See 1 more Smart Citation

Produção de celulases por fungos de ambiente marinho e terrestre para uso na hidrólise do bagaço de cana-de-açúcar e produção de 2,3-butanodiol pela bactéria <i>Serratia marcescens</i> a partir de glicose e glicerol

Santos¹

View full text Add to dashboard Cite

In Chapter 1 it is reported the cellulase production by 4 marine-derived fungi strains (Aspergillus sydowii CBMAI 934, A. sydowii CBMAI 935, Penicillium citrinum CBMAI 1186 and Mucor racemosus CBMAI 847) and 1 terrestrial fungi strain (Aspergillus sp. CBMAI 1198). They were grown in solid state fermentation using wheat straw as substrate (5 g) and with addition of peptone solution (0,75 g.L -1 ) enriched with inorganic salts. It was performed the enhancement of the growth conditions by changing the temperature, initial pH and moisture. The optimum temperature for all strains varied between 25-35 °C but A. sydowii CBMAI 935 with 40 °C. The optimum pH was different for each strain, varying from acidic to alkaline conditions. The optimum moisture content also varied accordingly the studied strain. In order enhance the cellulose hydrolysis performed by the produced cellulases, it was varied the pH, temperature and amount of the crude cellulase extract during the filter paper hydrolysis reaction. The obtained optimum values were different among strains and, in some cases, there were two optimum pH and temperature for the hydrolysis of the filter paper. Then, the obtained cellulases, using the best conditions for hydrolysis, were used in the sugarcane bagasse hydrolysis (10 FPU/g of sugarcane bagasse). The cellulases from the strains Aspergillus sp. CBMAI 1198 and A. sydowii CBMAI 934 were capable of degrading 75% and 78% of the sugarcane bagasse, respectively, generating reducing sugars. In Chapter 2, the capability of 6 strains (Bacillus subtilis LQOB-SE1, B. coagulans LQOB-SE2, B. pumillus LQOB-SE3, Brevibacillus brevis LQOB-SE4, Lysinibacillus sp. LQOB-SE5 and Serratia marcescens LQOB-SE6), isolated from peat soil, of producing 2,3-butanediol from glycerol fermentation. The only strain that produced 2,3-butanediol was S. marcescens LQOB-SE6, which was also applied in 2,3-butanediol production from glucose fermentation. Therefore, wastes from biodiesel and bioethanol production can be reused in industrial scale. The best conditions for glycerol fermentation: initial pH 7, Nutrient Broth (8 g. Tabela 3. Rendimento e produtividade obtidos de 2,3-butanodiol a partir da fermentação do glicerol pela bactéria S. marcescens LQOB-SE6 ao longo do tempo de cultivo. 182Tabela 4. Comparação entre os valores de rendimentos e produtividades obtidos na produção de 2,3-butanodiol a partir da fermentação do glicerol com resultados descritos na literatura para diferentes microrganismos. 183Tabela 5. Rendimentos e produtividades obtidos a partir da fermentação da glicose pela bactéria S. marcescens LQOB-SE6 ao longo do tempo de cultivo. 191Tabela 6. Dados de rendimentos e produtividades obtidos na produção de 2,3-butanodiol a partir da fermentação da glicose com microrganismos relatados na literatura e com a bactéria S. marcescens LQOB-SE6.

show abstract

Effect and characterization of sodium lignosulfonate on alkali pretreatment for enhancing enzymatic saccharification of corn stover

Cited by 71 publications

References 38 publications

Fenton pre-treatment of rice straw with citric acid as an iron chelate reagent for enhancing saccharification

Fenton pre-treatment of rice straw with citric acid as an iron chelate reagent for enhancing saccharification

Optimization of Pilot Scale Mechanical Disk Refining for Improvements in Enzymatic Digestibility of Pretreated Hardwood Lignocellulosics

Produção de celulases por fungos de ambiente marinho e terrestre para uso na hidrólise do bagaço de cana-de-açúcar e produção de 2,3-butanodiol pela bactéria <i>Serratia marcescens</i> a partir de glicose e glicerol

Contact Info

Product

Resources

About