Efek suplementasi β-carotene terhadap kolesterol total, trigliserida dan malondialdehid pada tikus sprague dawley yang diabet

Abstract

Background : diabetes mellitus (DM) is associated with increased total cholesterol and triglyceride, also well characterized by increased malondialdehyde production. β-carotene has antioxidant activity, glycemic and lipid control. Objective : to analyze the effect of β-carotene on total cholesterol, triglyceride and MDA on diabetic Rattus norvegicus sprague dawley. Methods : thirty rats were randomly divided into 5 groups : 1 (STZ), 2 (STZ+β-carotene 1 mg/kg BW), 3 (STZ+β-carotene 10 mg/kg BW), 4 (STZ+β-carotene 20 mg/kg BW), 5 (normal). Streptozotocin induced intraperitoneal 40 mg/kg BW. β-carotene was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP, total cholesterol by CHOD-PAP, triglyceride by GPO and MDA by ELISA with TBARS methods. Hypothesis test used one way anova then followed by post hoc bonferroni to analyze the efficient dose effect. Results : there was a significant difference of total cholesterol (p=0.002) after β-carotene 10 mg/kg BW supplementation on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most efficient dose to lowering total cholesterol. There were significant differences of triglyceride (p=0.0001) and MDA (p=0.0001) after β-carotene 1, mg/kg BW, 10 mg/kg BW and 20 mg/kg BW supplementation on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most efficient dose to lowering triglyceride, while 20 mg/kg BW to lowering MDA. Conclusion : β-carotene 10 mg/kg BW is the most efficient dose to lowering total cholesterol and triglyceride. β-carotene 20 mg/kg BW is the most efficient dose to lowering MDA.

Blood Sugar, Haemoglobin and Malondialdehyde Levels in Diabetic White Rats Fed a Diet of Corn Flour Cookies

“…Importantly, C4 corn cookies made with crystalline coconut sugar and VCO were found to have the highest beta-carotene content (1877.9 ppm, see Table 1 ). As an antioxidant, beta-carotene can protect beta-pancreatic cells from the cytotoxicity caused by oxidative stress in DM [ 50 ]. According to Furusho et al [ 51 ], supplementation with beta-carotene at 20 mg/kg body weight significantly reduced the levels of ROS and increased the levels of antioxidant enzymes in diabetic rats.…”

Effectiveness of moringa oleifera on triglyceride levels in diabetic wistar rats (Rattus norvegicus) induced with streptozotocin (STZ)

“…Increased free fatty acids will increase the secretion of very-low-density lipoprotein (VLDL) accompanied by triglycerides and cholesterol. [14] According to Putri's research (2017), in diabetes conditions, there is impaired insulin secretion, which is one of the hormones that affects triglyceride levels. The insulin hormone can reduce blood triglyceride levels because insulin can prevent hydrolysis triglycerides.…”