Efeito do estradiol e da progesterona no desenvolvimento e na qualidade de embriões bovinos produzidos in vitro

Reis, Alisson Rodrigo Souza; Silva, Lílian Kátia Ximenes; Silva, Aluizio Otavio Almeida da; Sousa, J. S.; Vale, W. G.

doi:10.1590/s0102-09352010000600012

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Another important factor that may significantly affect their activity is the presence of hormones. It has been known that treatment of BOEC with estradiol alone or in combination with progesterone increases the quality of embryos produced in vitro (42). Thus, further studies should be designed to mimic an in vivo environment with the presence of hormones at specific phases.…”

Section: Discussionmentioning

confidence: 99%

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Kürüm¹,

Karahan²,

Kocamiş³

et al. 2019

Turk J Vet Anim Sci

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The oviduct creates optimal conditions for oocyte maturation, sperm capacitation, fertilization, and embryo and gamete transport (1). The different segments of the oviduct vary by means of effects on spermatozoon functions. The isthmus is the section of the oviduct which serves as a reservoir for sperm, while the ampulla is known as the fertilization area (2). The oviduct epithelial lining is composed of two kinds of cells: ciliated and secretory cells. The ciliated cells play a role in oocyte, spermatozoon, and embryo transport, while secretory cells secrete substances which are important in sperm function and early embryonal development (3). While the oviduct epithelium improves the vitality and motility of the sperm, it also fosters an environment for sperm capacitation (4). The secretions of oviduct epithelial cells vary over the cycle (2). Ovarian-derived steroids regulate oviduct physiology and reproductive events in the oviduct (5). Both estrogen and progesterone receptors are found in the bovine oviduct epithelium. Gamete maturation and breeding occur during estrogen-dominant phases of the cycle, while fertilization, early stages of clefts, and morphogenesis of Abstract: Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment.

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Section: Discussionmentioning

confidence: 99%

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Kürüm¹,

Karahan²,

Kocamiş³

et al. 2019

Turk J Vet Anim Sci

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“…Matsuo et al (78) used a 3-step oocyte culture system that mimicked temporal physiological changes in E 2 and P 4 , and reported that the treatment enhanced in vitro embryo development reflected as a greater blastocyst formation rate. Reis et al (79) cultured zygotes in the following groups (concentrations in μg/mL): control, 1 E 2 + 100 P 4 , 1 E 2 , and 100 P 4 . The authors reported that E 2 and P 4 together and E 2 alone resulted in greater hatching rates and improved embryo quality.…”

Section: Evidence On the Effect Of Hormonesmentioning

confidence: 99%

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

Lizarraga,

Gaxiola,

Castro del Campo

et al. 2024

Vet Mex OA

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We conducted a scoping review to 1) map and categorize published studies reporting interventions during the in vitro production of Bovine embryos and 2) qualitatively summarize the effects of treatments based on hormones, growth factors, sera, and reproductive fluids during oocyte maturation and embryo development. We searched electronic databases using keywords ('Bovine', 'embryo', 'blastocyst', 'oocyte', 'in vitro', 'quality') derived from the PIOS approach of the PRISMA statement. We identified 231 studies published during 2000−2021, with 133 being published between 2012 and 2021. The 231 studies were classified into four treatment categories: culture conditions (28), medium composition (45), bioactive supplements (79), and other additives (79), with 19 subcategories within these categories. A total of 77 studies included hormones, growth factors, sera, and reproductive fluids, among which 53 studies reported a positive effect on embryo production. Hormone-based treatments using melatonin, gonadotropins, and steroids were the most effective, followed by interventions assessing growth factors such as EGF, FGF, IGF-1, and BMP. These treatments improved oocyte competence, cytoplasmic/nuclear maturation, oocyte quality, and blastocyst development. Although free-serum media and synthetic/alternative products can partially or totally replace serum, it is sometimes necessary even at low concentrations. At high concentrations, hormones or growth factors may have detrimental effects on oocyte nuclear maturation, impair embryo development, and decrease embryo survival. Further meta-analyses are needed to estimate the intervention-specific effects. Future research should focus on interventions that have a positive effect and can be used extensively for Bovine production.

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Efeito do estradiol e da progesterona no desenvolvimento e na qualidade de embriões bovinos produzidos in vitro

Cited by 2 publications

References 24 publications

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

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