Efeito de defensivos agrícolas naturais e extratos vegetais sobre parâmetros biológicos de Metarhizium anisopliae (Metsch.) Sorok

Mamprim, Ana Paula; Alves, L. F. A.; Pinto, Fabiana Gisele da Silva; Formentini, Marina Andressa; Martins, C. C.; Bonini, Andréia Kusumota

doi:10.5433/1679-0359.2013v34n4p1451

Cited by 8 publications

(14 citation statements)

References 3 publications

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“…Viability of conidia, colony forming units, vegetative growth, conidia production, "T" values and compatibility of Metarhizium anisopliae (UNIOESTE 22) subjected to different homeopathic medicines (26 ± 1°C and 12-h photophase). This increase in CFU is a trend already observed by other authors in analyses of NP (commercial products, aqueous and alcoholic plant extracts) and biological parameters of M. anisopliae, which can likely be attributed to the degradation and utilization of substances present in the composition of products tested and may have been used as nutrients by the fungus Mamprim et al, 2013).…”

Section: Treatmentssupporting

confidence: 59%

“…Accordingly, it was found that some commercial products such as Ecolife® and Stubble-Aid® and others neem-oil-based affected conidia production and the vegetative growth of M. anisopliae (Marques et al, 2004;. Mamprim et al (2013) highlighted the negative effect of lime sulfur broth on all biological parameters of M. anisopliae, classified as moderately toxic. However, the same authors have shown that aqueous and alcoholic plant extracts of some insecticide plants have proved compatible to the fungal development.…”

Section: Introductionmentioning

confidence: 99%

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In vitro assay on homeopathic solutions on Metarhizium anisopliae (Metsch) Sorok (Ascomycota: Clavicipitaceae)

et al. 2016

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This study aimed to evaluate the in vitro effect of homeopathic solutions Arsenicum Album 24CH; Calcarea carbonica 30CH; Kali iodatum 100CH;Phosphorus 3CH; Silicea 30CH;Staphysagria 6, 30 and 100CH; Spodoptera frugiperda 30CH; Sulphur 100 and 200CH andThuya occidentalis 200CH on biological parameters of the fungus Metarhizium anisopliae . The solutions were diluted in sterile distilled water (0.1%) and were sprayed on the previously inoculated fungus on PDA culture medium. Germination, colony forming units, vegetative growth, conidial production and insecticidal activity of the fungus against larvae of Diatraea saccharalis (Lepidoptera: Crambidae) were evaluated. Homeopathic solutions did not affect negatively the parameters evaluated. Thus, all treatments were considered compatible to the fungusM. anisopliae.

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Section: Treatmentssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

In vitro assay on homeopathic solutions on Metarhizium anisopliae (Metsch) Sorok (Ascomycota: Clavicipitaceae)

et al. 2016

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“…After this period, filtering was performed in sterile cotton gauze and filter paper, and then the filtrate liquid was maintained at -10 °C (Mamprim et al, 2013).…”

Section: Product and Extracts Usedmentioning

confidence: 99%

“…Following this distilled water was added in a waste weight/volume ratio to obtain a 10% concentration. Subsequently, vacuum sterilization was performed with a 0.45 mm pore membrane and the material was stored at -10 °C (Mamprim et al, 2013).…”

Section: Product and Extracts Usedmentioning

confidence: 99%

“…The commercial disinfectant Peroxitane  1512 AL (peracetic acid, hydrogen peroxide, acetic acid, and stabilizers) at the concentrations of 1:200 (recommended concentration -RC), 2RC and 0.5 RC were assessed. The extracts were prepared according to the methods of De Bona (2012) and Mamprim et al (2013) to collect, dry, ground and storage of leaves.…”

Section: Product and Extracts Usedmentioning

confidence: 99%

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Effect of plant extracts and a disinfectant on biological parameters and pathogenicity of the fungus Beauveria bassiana (Bals.) Vuill. (Ascomycota: Cordycipitaceae)

2016

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The fungus Beauveria bassiana is naturally found in poultry houses and causes high rates of mortality in Alphitobius diaperinus. Laboratory and field experiments have shown the potential of this fungus as an insect control agent. However, in poultry houses, bacteria as Salmonella, can be found and have been studied alternative control methods for this pathogen. Thus, this study aimed to evaluate the effect of plant extracts and a disinfectant on the fungus Beauveria bassiana (strain Unioeste 4). Conidial viability, colony-forming unit (CFU) counts, vegetative growth, conidia production, insecticidal activity of the fungus and compatibility were used as parameters in the evaluation of the effect of these products on the fungus. Alcoholic and aqueous extracts of jabuticaba (Myrciaria cauliflora (Mart.), guava (Psidium guajava (L.)), and jambolan (Syzygium cumini (L.), at concentrations of 10% as well as the commercial disinfectant, Peroxitane  1512 AL, were evaluated at the recommended concentrations (RC), 1:200 (RC), 0.5 RC and 2 RC. There was a negative influence of alcoholic and aqueous extracts of jabuticaba, guava and three dilutions of Peroxitane on the viability of conidia. The CFUs and vegetative growth of the fungus were affected only by the Peroxitane (all dilutions). For conidial production, the aqueous extract of guava had a positive effect, increasing production, while the Peroxitane at the R and RC concentrations resulted in a negative influence. The mortality of A. diaperinus, caused by the fungus after exposure to these products, was 60% for the peracetic acid at 0.5 RC, and above 80% for the extracts. Thus, the results showed that all the extracts and Peroxitane at RC 0.5 are compatible with the fungus B. bassiana Unioeste 4, however only the extracts had a low impact on inoculum potential. Keywords: Myrciaria cauliflora), na concentração de 10% e também o desinfetante comercial Peroxitane  1512 AL na concentração recomendada -1:200 (CR), 0,5 CR e 2CR. Foram avaliados a: germinação dos conídios, unidades formadoras de colônias (UFC), crescimento vegetativo, produção de conídios e efeito sobre a atividade inseticida do fungo contra adultos de A. diaperinus, bem como a compatibilidade entre produtos e o fungo. Verificou-se influência negativa dos extratos alcoólico e aquoso de jabuticabeira, goiabeira e das três diluições de Peroxitane sobre a viabilidade dos conídios. Já, a UFC e o crescimento vegetativo foram afetados apenas com Peroxitane (em todas as diluições). Para produção de conídios, o extrato aquoso de goiabeira teve efeito positivo, elevando a produção, enquanto as diluições recomendada e o dobro de Peroxitane mostraram influência negativa. Observou-se ainda que a mortalidade de A. diaperinus causada pelo fungo após a exposição aos produtos foi de 60% para o ácido peracético na 0,5 CR, já para os extratos foi acima de 80%. Assim, os resultados demonstraram que todos os extratos e o Peroxitane na 0,5 CR são compatíveis com o fungo B. bassiana Unioeste 4, porém apenas os extratos...

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Effect of nutraceuticals on acanthocephalan Neoechinorhynchus buttnerae and its toxicity to the host tambaqui Colossoma macropomum

et al. 2019

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The production of tambaqui Colossoma macropomum has been undergoing financial losses due to parasitic infection by the acanthocephalan Neoechinorhynchus buttnerae, raising an alert for aquaculture in South America. The lack of adequate treatment and use of unlicensed chemicals encourages research for alternative solutions with minimal side effects. The objectives of this study were to evaluate the in vitro antiparasitic potential of commercial nutraceutical products (Natumix® and BioFish®) against N. buttnerae and to assess the respective in vivo toxic effects on the host tambaqui. For in vitro assays, parasitized fish were necropsied for acanthocephalans sampling. The parasites were exposed to three concentrations (0.078, 0.313 and 1.25 mg/ml) of each product, as well as controls (one without product and another with a solubilizer). For the in vivo acute toxicity test, juvenile fish (<0.1 g) were exposed to five increasing concentrations of each product. Mortality of tambaqui was recorded at 24, 48, 72 and 96 h. The estimated lethal concentration (LC) for 10, 50, 90 and 99% of fish was determined to classify the toxicity of the products on the target species. After in vitro efficacy tests, the highest concentrations (1.25 mg/ml) caused 100% mortality of the parasites in both products, but only Natumix® caused 100% mortality using the intermediate concentration (0.313 mg/ml) after 24 h. According to the acute toxicity result, the LC50 classified the nutraceutical products as slightly toxic for tambaqui. The tested products had a parasiticidal effect on N. buttnerae, and the toxicity test showed that both products have therapeutic potential when added to the diet.

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Efeito de defensivos agrícolas naturais e extratos vegetais sobre parâmetros biológicos de Metarhizium anisopliae (Metsch.) Sorok

Cited by 8 publications

References 3 publications

In vitro assay on homeopathic solutions on Metarhizium anisopliae (Metsch) Sorok (Ascomycota: Clavicipitaceae)

In vitro assay on homeopathic solutions on Metarhizium anisopliae (Metsch) Sorok (Ascomycota: Clavicipitaceae)

Effect of plant extracts and a disinfectant on biological parameters and pathogenicity of the fungus Beauveria bassiana (Bals.) Vuill. (Ascomycota: Cordycipitaceae)

Effect of nutraceuticals on acanthocephalan Neoechinorhynchus buttnerae and its toxicity to the host tambaqui Colossoma macropomum

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