Efeito da cisteína adicionada ao meio diluente sobre a cinética de espermatozóides ovinos após descongelamento

Corandin, Eduardo Mazon; Pereira, Pedro Henrique Scarpelli; Prado, Tayrone Freitas; Filho, B. D. Oliveira; Meirinhos, Pedro Gambarini; Gambarini, M. L.

doi:10.5433/2316-5200.2013v2n3espp21

Cited by 2 publications

(3 citation statements)

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“…In their study on pig sperm, Broekhuijse et al (2012) demonstrated that progressive motility, VCL, and BCF are related to the frequency of deliveries; and that the total motility, VAP, VSL, and ALH affected slightly but significantly (P < 0.05) the number of piglets born. In sheep, the kinetic variables VAP, VCL, and VSL have been associated with the increased ability of sperm to reach the fertilization site and fertilize the egg (CORANDIN et al, 2013). However, since no well-defined cause and effect trend was observed between the composition of the diluting extenders and between the preservation methods with the kinetic attributes in this experiment, we cannot infer whether diluting extenders with LDL would result in a higher or lower fertilization probability.…”

Section: Discussionmentioning

confidence: 38%

“…Furthermore, the reduction in the activity of these two enzymes was higher using an egg yolk-based diluting extender than in extenders with LDL. Corandin et al (2013) showed that diluting extenders with LDL instead of whole egg yolk reduced the negative effects caused by the cooling and freezing processes on the activity of the antioxidant enzymes from ram spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

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Low density lipoproteins added to an extender frozen or lyophilized are evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added

et al. 2015

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Low density lipoproteins added to an extender frozen or lyophilizedare evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added AbstractThe aim of this study was to test different concentrations of low density lipoprotein (LDL) in replacement of whole egg yolk in extenders preserved in the aqueous or lyophilized form, for ram sperm cryopreservation using two freezing curves (-40°C/min from 5 to -140°C and nitrogen vapor). One ejaculate from six Santa Inês rams was collected. Each ejaculate was divided into nine different diluents as follows: Tris-16% yolk (control), and Tris with 2, 4, 6 and 8% fresh LDL, and criopreserved in the aqueous or lyophilized form. The samples were diluted to a final concentration of 100 x 10 6 sperm/mL and filled into 0.25 ml straws. After thaw, sperm cells were evaluated for motility and sperm kinetics (CASA), and submitted to the hypoosmotic swelling test and the evaluation of the structural integrity of sperm membranes using fluorescent dyes (CFDA: PI), as well as sperm morphology and longevity. The experimental design was randomized blocks, and results were submitted to ANOVA and the averages were compared using the Scott-Knott test. There were no differences in progressive motility and functional and structural integrity of the membrane evaluated when different concentrations of aqueous or lyophilized low density lipoproteins or egg yolk were added to the extender (P>0.05). As for the velocity of sperm movement, the control medium had some kinetic scores similar to the extender containing LDL, both aqueous and lyophilized (P> 0.05). Results were similar between cooling curves. Therefore, we conclude that the media containing all concentrations of LDL, aqueous or lyophilized, were able to protect the ram sperm cells during the cryopreservation process, as whole egg yolk did. ResumoO objetivo deste trabalho foi testar para criopreservação de sêmen ovino diferentes concentrações de lipoproteína de baixa densidade (LBD) em substituição à gema de ovo em meios diluidores, armazenados na forma aquosa e liofilizada, utilizando-se duas curvas de congelação (-40°C/min de 5 à -140°C ou vapor de nitrogênio). Os ejaculados de seis carneiros da raça Santa Inês foram fracionados e distribuídos em nove tratamentos, sendo o primeiro o meio controle (Tris-gema 16%) e os demais meios Tris contendo lipoproteínas de baixa densidade nas concentrações de 2, 4, 6 e 8% (v/v), criopreservados tanto na forma aquosa quanto liofilizada. As amostras foram diluídas para a concentração final de 100 x 10 6 espermatozoides/mL e envasadas em palhetas de 0,25 mL. Após a descongelação foram avaliadas a motilidade e cinética espermática (CASA), morfologia e longevidade espermáticas, além da integridade funcional (HOST) e estrutural (CFDA/IP) das membranas. O delineamento experimental foi blocos ao acaso e os resultados foram submetidos a ANOVA, sendo as médias comparadas pelo teste de Scott-Knott. Quanto aos parâmetros de motilidade progressiva e integridade funcional e estrutural...

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Section: Discussionmentioning

confidence: 38%

Section: Discussionmentioning

confidence: 99%

Low density lipoproteins added to an extender frozen or lyophilized are evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added

et al. 2015

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“…Zootec., v.70, n.5, p.1547-1556, 2018 For the parameters curvilinear velocity (CLV) and straightness (STR), the results found in this study were higher than the 173.4±4.33 and 77.1±1.56, respectively, published by Sicherle (2007), who added catalase to the extender, and similar when the author added trolox (179.2 ± 5.98 and 80.5±1.29). Regarding average path velocity (APV), amplitude of lateral head displacement (ALH), and straight-line velocity (SLV), the results of this study were also superior to those found by Corandin et al (2013), who added 2.5mM cysteine to the extender. However, the linearity of the sperm diluted in extender with aqueous extract of noni was lower than that of the afore-mentioned studies.…”

Section: Discussioncontrasting

confidence: 60%

Use of aqueous extract of noni in extender for sheep semen freezing

Nascimento

Azevedo

Velarde

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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The study aimed to evaluate the action of aqueous extract of noni in an extender for sheep semen freezing. Treatments differed in inclusion of aqueous extract of noni in the extender: T1 ˗ no addition; T2 ˗ 24µg/mL; T3 ˗ 72µg/mL; and T4 ˗ 120µg/mL. Ejaculates were collected, diluted in the four treatments, and frozen. After thawing, the semen was subjected to a thermoresistance test and evaluated for subjective motility, vigor, membrane integrity assessment by hypo-osmotic swelling test, live-dead assay, computer-assisted sperm analysis and the status of sperm capacitation and acrosome reaction. Data were subjected to ANOVA, and then to Student Newman Keuls’s test at 5% significance level. In the thermoresistance test after two hours of incubation, motility in T4 (120µg/mL) was lower than in the other treatments, with no differences in the HoS test in either diluted semen or in the semen evaluated immediately post-thawing, while for the other times, treatments showed similar responses. Regarding the motility parameters, a difference was observed for progressive motility, curvilinear velocity, average path velocity, and amplitude of lateral head displacement. As to the sperm capacitation status, a difference was observed between treatments for the sperm capacitated with intact acrosome.

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Efeito da cisteína adicionada ao meio diluente sobre a cinética de espermatozóides ovinos após descongelamento

Cited by 2 publications

References 0 publications

Low density lipoproteins added to an extender frozen or lyophilized are evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added

Low density lipoproteins added to an extender frozen or lyophilized are evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added

Use of aqueous extract of noni in extender for sheep semen freezing

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