Efectos farmacologicos y enzimaticos de los venenos de serpientes de Antioquia y Choco (Colombia)

Otero, Rafael; Osorio, R.G.; Valderrama, Rafael; Giraldo, César Augusto Giraldo

doi:10.1016/0041-0101(92)90855-y

Cited by 53 publications

(44 citation statements)

References 10 publications

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“…The hemolytic activity was concentration-dependent and thermolabile since heating the venom at ≥ 40°C resulted in little or no hemolysis. These findings generally agree with reports on the hemolytic activity of other Bothrops venoms (14)(15)(16). Variation in species sensitivity to hemolysis as seen herein (sheep < cow/rat < horse) has also been observed with other Bothrops venoms, e.g., B. asper venom causes direct hemolysis of mouse erythrocytes but has no effect on goat, horse, rabbit, sheep and toad erythrocytes; in contrast, lysis of human erythrocytes requires bovine serum albumin and Ca 2+ (17).…”

Section: Discussionsupporting

confidence: 82%

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom

Martins¹,

Araújo²,

Bon³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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Bothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A 2 (PLA 2 ). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 µg/mL) and markedly attenuated by heating the venom for 30 minutes at ≥ 40°C and by the PLA 2 inhibitor p-bromophenacyl bromide. An acidic PLA 2 (5 µg/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA 2 showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the enzymatic and hemolytic activities of this enzyme may be neutralized during antivenom therapy. These results indicate that B. lanceolatus venom and its PLA 2 can cause hemolysis in vitro.

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Section: Discussionsupporting

confidence: 82%

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom

Martins¹,

Araújo²,

Bon³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Therefore, the absence of hemorrhagic activity in these venoms may suggest that the edema is induced by proteins different from these proteinases with edematous activity like myotoxins and phospholipases A 2 (19,20,30). In previous studies with C. durissus venoms from other South American regions, no edematous or hemorrhagic activity was observed (9)(10)(11)31). This is in contrast to the positive results reported for C. durissus snakes from Central and North America (11).…”

Section: Discussioncontrasting

confidence: 55%

Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents

Céspedes

Castro

Jimenez³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:Crotalus durissus cumanensis, a rattlesnake endemic to Colombia and Venezuela, is considered one of the most lethal snake species in Latin America. The aim of the present study was to compare the protein content and biological activity of the venom obtained from eight specimens of C. durissus cumanensis, namely two adults from different localities of Colombia and six offspring born in captivity. Protein profiles of crude venoms were analyzed by SDS-PAGE and RP-HPLC, and biological activities were evaluated for lethality, edema, defibrination, hemolytic and coagulant activities to assess individual venoms of adults and a pool of young snake venoms. Transient edema appeared rapidly after venom inoculation, whereas hemorrhagic effect was not observed. Differences in protein profiles, lethality, hemolytic, coagulant and defibrinating activities between both adult snake venoms were observed; those from the mother snake exhibited higher activities. Venoms from young snakes were similar to the one obtained from the mother, but the coagulant effect was stronger in offspring venoms. Notably, biological effects of the father snake venom were not comparable to those previously described for C. durissus cumanensis from Venezuela and C. durissus terrificus from Brazil, confirming the high variability of the venom from Crotalus species.

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“…[17][18][19] Esses venenos induzem reações locais imediatas como dor, edema, hemorragia e mionecrose, 20 podendo levar à perda tecidual abundante, assim como de parte ou de todo o membro atingido. Dentre as atividades biológicas sistêmicas destacam-se principalmente a coagulante e a hemorrágica.…”

Section: Introductionunclassified

Constituintes químicos e atividade antiedematogênica de Peltodon radicans (Lamiaceae)

Costa¹,

Dos-Santos²,

Alcântara³

et al. 2008

Quím. Nova

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ArtigoDedicamos este trabalho à Helena Ferraz, que nasceu e cresceu, e que me viu também nascer e crescer, em um daqueles muitos rincões mineiros encostados na Serra da Mantiqueira. Mantiqueira -do tupi "Amantikir" -a montanha que chora, agora chora e choramos ainda mais, por você, Helena. *e-mail: aalcantara@zeus.qui.ufmg.br Recebido em 4/6/07; aceito em 11/10/07; publicado na web em 10/3/08 CHEMICAL CONSTITUENTS AND ANTIEDEMATOGENIC ACTIVITY OF Peltodon radicans (Lamiaceae). Most of the snakebite incidents in the Amazon region involve Bothrops atrox, whose venom presents the most potent edematogenic and necrotic activities in the genus. This work describes the studies of isolation of the chemical constituents and antiedematogenic activity of the species Peltodon radicans (Lamiaceae), which is used in the treatment of snakebites and scorpion stings in the region. The extracts presented aliphatic hydrocarbons, 3β-OH,β-amirin (1), 3β-OH,α-amirin (2), β-sitosterol (3), stigmasterol (4), ursolic acid (5), 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid (tormentic acid, 6), methyl 3β-hydroxy,28-methyl-ursolate (7), sitosterol-3-O-β-D-glucopyranoside (8), and stigmasterol-3-O-β-D-glucopyranoside (9). The flower extracts presented the higher antiedematogenic activity. This is the first report on the study of the flowers, stem, and roots of this plant. CONSTITUINTES QUÍMICOS E ATIVIDADE ANTIEDEMATOGÊNICA DE Peltodon radicans (LAMIACEAE)HabdelKeywords: Peltodon radicans; antiedematogenic activity; triterpenes. INTRODUÇÃOSegundo a Organização Mundial da Saúde, anualmente cerca de 2,5 milhões de pessoas sofrem acidentes ofídicos no mundo, com 125 mil mortes.1 Esses acidentes representam um enorme problema de saúde pública, especialmente em países tropicais.2-4 Na África, são registrados cerca de 500 mil acidentados por ano, com 40% dos casos hospitalizados e 20.000 óbitos.5 Na Ásia há entre 25 e 35 mil óbitos por ano.6 O Ministério da Saúde do Brasil registra entre 19 e 22 mil acidentes ofídicos por ano, com aproximadamente 0,45% de letalidade.7 Quando informado o gênero da serpente, Bothrops foi responsável por 90,5% dos acidentes ofídicos, envolvendo 0,31% de óbito.Bothrops atrox (jararaca) é encontrada vastamente na região Norte do Brasil, 8,9 habitando ambientes úmidos de zonas rurais ou periféricas das grandes cidades, onde haja facilidade para proliferação de roedores.10-13 Na Amazônia essa espécie ocorre em altas densidades, principalmente em florestas, apresentando hábitos noturnos. Os jovens ingerem presas exotérmicas e os adultos, presas endotérmicas, ocorrendo portanto variação ontogenética para a peçonha dessa espécie.14-16 Os venenos de B. atrox são constituídos principalmente por proteínas com atividade enzimática, apresentando variações tanto inter quanto intraespecíficas. [17][18][19] Esses venenos induzem reações locais imediatas como dor, edema, hemorragia e mionecrose, 20 podendo levar à perda tecidual abundante, assim como de parte ou de todo o membro atingido. Dentre as atividades biológicas sistêmicas des...

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Efectos farmacologicos y enzimaticos de los venenos de serpientes de Antioquia y Choco (Colombia)

Cited by 53 publications

References 10 publications

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom

Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents

Constituintes químicos e atividade antiedematogênica de Peltodon radicans (Lamiaceae)

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