<p><strong>Background</strong>: Peru is considered the third largest exporter of mango in the world and Piura is the first mango producing region representing 68% of the national production; however, the fungus <em>Lasiodiplodia theobromae</em> has been associated with dieback and the presence of cankers in mango cultivation, reducing fruit production and quality. <strong>Objective</strong>: To evaluate sustainable alternatives for <em>in vitro</em> and <em>in vivo</em> control of <em>L. theobromae</em> in mango, using products based on copper sulfate pentahydrate and a liquid biofertilizer. <strong>Methodology</strong>: Treatments were applied: T0 (Control), T1 (Vacun-Q organic 0.35 mL100 mL<sup>-1</sup>), T2 (Vacun-Q organic 0.175 mL 100 mL<sup>-1</sup>), T3 (Phyton 27® 0.35 mL100 mL<sup>-1</sup>), T4 (Phyton 27® 0.175 mL100 mL<sup>-1</sup>), T5 (Biol 10%) and T6 (Biol 20%). Radial growth and mycelial growth inhibition percentage (GIP) of the pathogen were evaluated in the <em>in vitro</em> phase with poisoned media, and necrotic area progression was assessed in the <em>in vivo </em>phase on mango leaves. <strong>Results</strong>: At 72 h after inoculation, treatments T3, T5 and T6 showed the lowest radial growth of <em>L. theobromae</em>, statistically equal to each other and inferior to the control that completed its development in the Petri dish; likewise, they showed 100% GIP. Five days after inoculation of mango leaves, T5 and T6 presented the smallest necrotic areas with 1.44 cm2 and 1.61 cm2 respectively, followed by T4 with 1.64 cm<sup>2</sup> and T2 with 1.69 cm<sup>2</sup> compared to the control with 2.05 cm<sup>2</sup>. <strong>Implications</strong>: In view of the constant restrictions of active molecules, sustainable and innocuous alternatives for humans and the environment are sought for the control of <em>L. theobromae</em>. <strong>Conclusion</strong>: Treatments T5 and T6 based on liquid biofertilizers showed to be effective in the <em>in vitro</em> and <em>in vivo</em> control of <em>L. theobromae</em>.</p>