Efecto del volumen de empaque sobre la tasa de congelación-descongelación y la fertilidad de semen crioconservado de yamú (Brycon amazonicus)

Abstract

SUMMARYThe aim of this study was to evaluate the freezing-thawing conditions of yamú (Brycon amazonicus) sperm packaged in straws of different volume and their effect on the spermatic motility and fertility post-thawing. The induction to gonadal final maturation was made with carp pituitary extract. The sperm obtained was evaluated and diluted (1:4) in a solution with 5.5% glucose, 12% egg yolk and 10% dimethyl sulfoxide, packaged in 0.5, 1.8, 2.5 or 4.0 ml straws and frozen in nitrogen vapors. The thawing was carried out in a 35ºC, 60ºC or 80ºC water bath during different times. All straws showed a similar overall freezing rate (7.5 to 12.9ºC min -1 ). On the other hand, the overall thawing rate was faster at a higher water bath temperature. The 4.0 ml straws thawed at 35ºC showed the highest motility and activation time post-thaw (47.0 ± 1.6% and 60.2 ± 2.4 sec). The fertility obtained with cryopreserved sperm in all straw sizes was significantly lower (P < 0.05) than with fresh sperm (65 ± 4%). The highest fertility was observed with 0.5 ml straw thawed at 35ºC (52 ± 3%), followed by 2.5, 4.0 and 1.8 ml straws (51 ± 3, 48 ± 3 and 47 ± 3%, respectively). It was concluded that 4.0 ml straws thawed at 35ºC showed the best post-thaw sperm quality and more homogenous freezing-thawing rates in comparison to the other packaging systems. On the other hand, no effects on fertility were observed in all straw sizes thawed at 35 ºC.Palabras clave: fertilidad, pajilla, semen, tasa de congelación-descongelación.

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

“…After approximately 10 min of equilibration, the support was transferred into a dry shipper (CP100 Taylor‐Wharton, Theodore, Alabama, USA) for 30 min. The freezing rate was ~7.5ºC/min (Medina‐Robles et al 2007). Finally, the straws were stored for 15 d in liquid nitrogen in a cryogenic tank (Taylor‐Wharton HC 35).…”

Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae (Pisces, Characiformes)

“…Nevertheless, when P. magdalenae had rapid freezing (like P. lineatus) and rapid thawing (60°C for 8 seconds) there were total motilities of up to 54.93% on average, which significantly changed when a slow thawing rate was used (0% of total motility). Similarly, for another Characiforme such as Yamú Brycon amazonicus, a rapid freezing rate with a slow thawing rate generates higher total motilities (35.3%) than those originated when rapid thawing rates were used (7.2%) (25). This is evidently opposite to what results with P. magdalenae, where the rapid thawing always generates the greatest results.…”

Crioconservación seminal de Colossoma macropomum como estrategia de producción y conservación en la Orinoquia Colombiana

“…Con respecto a la evaluación de la motilidad postdescongelación, no fue observado un comportamiento homogéneo en el semen diluido y empacado en pajillas de 0.5 y 2.0 ml. Lo anterior, indica que el sistema de empaque podría tener incidencia en los resultados de motilidad postdescongelación para la especie de estudio como ha sido observado en otros trabajos (Medina et al, 2007;Ramírez-Merlano et al, 2011). Esto contrasta con los estudios realizados por María et al, (2015), donde se evaluaron empaques con diferentes volúmenes (1.6 y 4.5 ml) sin diferencias significativas para esta misma característica seminal.…”