EF-1&amp;#945; gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains

Silva, Fabiana Pires da; Vechiato, M. H.; Harakava, Ricardo

doi:10.1590/s1982-56762014000100008

Cited by 23 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The F1 fragment was firstly used in the Elata Clade of the genus Morchella for population studies and some potential hybridization or gene transfer events were revealed in several species (Du et al 2016). The phylogeny of intergenic spacer region (IGS) was reported to conflict with the phylogeny of EF1-a in the genus Fusarium (Mbofung et al 2006;Silva et al 2014). By comparing the reproductive (MAT1-1-1 and MAT1-2-1) and the non-reproductive (F1 and IGS) datasets, we tested whether the conflicts are inherent only to the reproductive genes or they also exist in other parts of the genome in species of the Esculenta Clade.…”

Section: Conflicts Between Non-reproductive Genes (F1 and Igs) And Thmentioning

confidence: 99%

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

Kang

et al. 2020

IMA Fungus

View full text Add to dashboard Cite

Mating-type genes are central to sexual reproduction in ascomycete fungi and result in the establishment of reproductive barriers. Together with hybridization, they both play important roles in the evolution of fungi. Recently, potential hybridization events and MAT genes were separately found in the Elata Clade of Morchella. Herein, we characterized the MAT1-1-1 and MAT1-2-1 genes of twenty-two species in the Esculenta Clade, another main group in the genus Morchella, and proved heterothallism to be the predominant mating strategy among the twenty-two species tested. Ascospores of these species were multi-nuclear and had many mitochondrial nucleoids. The number of ascospore nuclei might be positively related with the species distribution range. Phylogenetic analyses of MAT1-1-1, MAT1-2-1, intergenic spacer (IGS), and partial histone acetyltransferase ELP3 (F1) were performed and compared with the species phylogeny framework derived from the ribosomal internal transcribed spacer region (ITS) and translation elongation factor 1-alpha (EF1-a) to evaluate their species delimitation ability and investigate potential hybridization events. Conflicting topologies among these genes genealogies and the species phylogeny were revealed and hybridization events were detected between several species. Different evolutionary patterns were suggested for MAT genes between the Esculenta and the Elata Clades. Complex evolutionary trajectories of MAT1-1-1, MAT1-2-1, F1 and IGS in the Esculenta Clade were highlighted. These findings contribute to a better understanding of the importance of hybridization and gene transfer in Morchella and especially for the appearance of reproductive modes during its evolutionary process.

show abstract

Section: Conflicts Between Non-reproductive Genes (F1 and Igs) And Thmentioning

confidence: 99%

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

Kang

et al. 2020

IMA Fungus

View full text Add to dashboard Cite

show abstract

“…One of these patients presented isolates of 2 different species one of which was related to the environmental isolates (F.oxysporum). We believe that the typing method used in our study was discriminatory [14,22].…”

Section: Discussionmentioning

confidence: 67%

An outbreak of invasive fusariosis in a children’s cancer hospital

Litvinov

Silva

Heijden

et al. 2015

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Fusarium is considered an emerging pathogen, and there are few reports of fusariosis in children. The objective of this study was to describe an outbreak of invasive fusariosis in a children's cancer hospital. A neutropenic 17-year-old male patient hospitalized for 10 days for a relapse of acute myeloid leukaemia, under chemotherapy, presented fever without any other symptoms; a thoracic computerized tomography showed bilateral pulmonary nodules. During voriconazole treatment, 1-cm reddened and painful subcutaneous nodules appeared on arms and legs and the culture of a skin biopsy revealed F. solani. Another case occurred 11 days later and started an outbreak investigation. Water samples for cultures were collected from taps, showers and water reservoirs. Air from all patient rooms was sampled. Faucets and the drains of sinks and showers were swabbed and cultured. Environmental and clinical isolates were typed. There were 10 confirmed cases of infection caused by Fusarium spp. F. oxysporum and F. solani were isolated from water, swabs and air in patient rooms. Many control measures were instituted, but the outbreak was only controlled 1 year after the first case, when water filters filtering 0.2 μm were installed at the exit of all faucets and showers in all patient rooms (points-of-use). Typing demonstrated that clinical isolates of F. oxysporum were similar to those of the environment. In conclusion, to our knowledge this is the first reported outbreak of invasive fusariosis in children with oncohaematologic disease. It was controlled using 0.2-μm filters in all tap faucets and showers.

show abstract

“…Molecular identification of Fusarium species complex and species were performed by sequencing the internal transcribed spacer (ITS) region of ribosomal DNA [29] and the elongation factor 1 alpha (EF-1α) gene, respectively [30]. Mycelia were produced by inoculating microconidia of clinical isolates into Sabouraud-dextrose broth media and incubated at 28 • C at 120 rpm (MAX Q 4000 Benchtop Orbital Shaker Incubator (Thermo Fisher Scientific, Waltham, MA, USA) for 3 days.…”

Section: Molecular Identification Of Fusarium Sppmentioning

confidence: 99%

“…The polymerase chain reactions (PCR) amplifications were carried out with TransTaq ® DNA Polymerase High Fidelity (TransGen Biotech, Beijing, China) and primers ITS1 (5 TCC GTA GGT GAA CCT GCG G 3 ) and ITS4 (5 TCC TCC GCT TAT TGA TAT GC 3 ) [29]; and EF-1α forward (5 ATG GGT AAG GA(A/G) GAC AAG AC 3 ) and EF-1α reverse (5 GGA (G/A)GT ACC AGT (G/C)AT CAT GTT-3') [30]. Purified PCR products were sequenced with the respective primers in ABI 3730 DNA Analyzer (Life Technologies-Applied Biosystems, Waltham, MA, USA).…”

Section: Molecular Identification Of Fusarium Sppmentioning

confidence: 99%

See 1 more Smart Citation

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

View full text Add to dashboard Cite

The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.

show abstract

EF-1α gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains

Cited by 23 publications

References 26 publications

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

An outbreak of invasive fusariosis in a children’s cancer hospital

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Contact Info

Product

Resources

About