Edwardsiellosis in fish: a brief review

Mohanty, B.R.; Sahoo, P. K.

doi:10.1007/s12038-007-0125-x

Cited by 80 publications

(106 citation statements)

References 102 publications

Supporting

Mentioning

103

Contrasting

Unclassified

Order By: Relevance

“…Edwardsiella tarda is considered as an important pathogen of fish and has been isolated from a variety of cultured fishes, including eel, channel catfish, mullet, seabream, chinook salmon, tilapia, tropical fish black skirt tetra and angelfish (Austin and Austin 2007; Mohanty and Sahoo 2007). Recently, Edw.…”

Section: Introductionmentioning

confidence: 99%

Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4

Yin

et al. 2010

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: The aim of this study was to elucidate the potential quorum‐sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB‐4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double‐layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N‐acylhomoserine lactone (AHL)‐related compounds in bacteria. LTB‐4 was found to have two QS systems, one was based on the AHLs and the other was based on the autoinducer‐2 (AI‐2). The AI‐2 activity produced by LTB‐4 was growth phase dependent and topped at OD600 of 1·0. The protocol to detect cholerae autoinducer 1 (CAI‐1) activity in bacteria was modified, lowering the background luminescence of biosensor strain Vibrio harveyi JAF375. CAI‐1 activity could not be detected in LTB‐4. Conclusion: Edwardsiella tarda LTB‐4 produced at least four kinds of AHLs during its whole growth phase. In comparison with the AHL‐inducing QS, AI‐2 may be the first predominant signal, functioning at early exponential phase. LTB‐4 did not produce any CAI‐1 activity. Significance and Impact of the Study: Different QS signal molecules of Edw. tarda LTB‐4 were clarified by improved bioassays. In contrast to earlier studies detecting two types of AHLs, strain LTB‐4 produced at least four kinds of AHLs, which seemed to be C4‐HSL, C6‐HSL, 3‐oxo‐C6‐HSL and an uncharacterized AHL molecule.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4

Yin

et al. 2010

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Edwardsiella tarda is a Gram‐negative bacterium and a causative agent of septicaemia in freshwater and marine fish which is responsible for significant economic loss in the aquaculture industry (Thune et al. 1993; Abbott and Janda 2006; Mohanty and Sahoo 2007; Ye et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of mltA in Edwardsiella tarda reduces resistance to antibiotics and enhances lethality in zebra fish

Liu

Dong

Zhang

2012

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: The aim of this study was to investigate the role of membrane‐bound lytic murein transglycosylase A (MltA) in a bacterial fish pathogen Edwardsiella tarda. Methods and Results: An mltA in‐frame deletion mutant (ΔmltA) and an mltA overexpression strain (mltA+) of Edw. tarda were constructed through double‐crossover allelic exchange and by transformation of a low‐copy plasmid carrying the intact mltA into the ΔmltA mutant, respectively. Either inactivation or overexpression of MltA in Edw. tarda resulted in elevated sensitivity to β‐lactam antibiotics and lower viability in oligotrophic or high osmotic environment than wild‐type strain. Autolysis induced by EDTA was reduced in ΔmltA strain, while mltA+ strain was virtually flimsy, indicating that MltA is responsible for the lysis effect. Moreover, mltA+ strain exhibited significant increases in lipopolysaccharide (LPS) biosynthesis and virulence to zebra fish compared with wild‐type strain. Conclusions: The results indicated that MltA plays essential roles in β‐lactam antibiotics and environmental stresses resistance, autolysis, LPS biosynthesis and pathogenicity of Edw. tarda. This is the first report that MltA has a virulence‐related function in Edw. tarda. Significance and Impact of the Study: This study provided useful information for further studies on pathogenesis of Edw. tarda.

show abstract

“…2005; Castro et al. 2006; Agnew and Barnes 2007; Mohanty and Sahoo 2007). In China, both species are severe pathogens to a large number of economic fish species including Japanese flounder ( Paralichthys olivaceus ) and turbot ( Scophthalmus maximus ) (Zhang et al.…”

Section: Introductionmentioning

confidence: 99%

AStreptococcus iniaeDNA vaccine delivered by a live attenuatedEdwardsiella tardavia natural infection induces cross-genus protection

Sun

Liu

et al. 2012

Letters in Applied Microbiology

View full text Add to dashboard Cite

Edwardsiella tarda and Streptococcus iniae are important fish pathogens. We have reported previously a live E. tarda vaccine based on the attenuated strain TX5RM and a S. iniae DNA vaccine based on the antigen Sia10. In this study, we examined the possibility of constructing a cross‐genus vaccine by taking advantage of the residual infectivity of TX5RM and using it as a carrier host for the natural delivery of a S. iniae DNA vaccine. For this purpose, the recombinant TX5RM, TX5RMS10, was created, which harbours and retains stably the DNA vaccine plasmid pCS10 that expresses Sia10. When flounder were vaccinated with TX5RMS10 via oral and immersion routes, TX5RMS10 was detected in multiple tissues within 12–14 days postvaccination (p.v.). At 7 and 14 days p.v., expression of the DNA vaccine was detected in spleen, kidney and liver. Following E. tarda and S. iniae challenge at one and 2 months p.v., the vaccinated fish exhibited relative per cent survival rates of 69–83%. Immunological analysis indicated that TX5RMS10‐vaccinated fish produced specific serum antibodies and exhibited enhanced expression of a wide range of immune genes.

show abstract

Edwardsiellosis in fish: a brief review

Cited by 80 publications

References 102 publications

Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4

Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4

Overexpression of mltA in Edwardsiella tarda reduces resistance to antibiotics and enhances lethality in zebra fish

AStreptococcus iniaeDNA vaccine delivered by a live attenuatedEdwardsiella tardavia natural infection induces cross-genus protection

Contact Info

Product

Resources

About