Edwardsiellosis in fish: a brief review

Mohanty, B.R.; Sahoo, P. K.

doi:10.1007/s12038-007-0143-8

Cited by 338 publications

(139 citation statements)

References 103 publications

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“…This growth temperature profile of fish is in agreement with our findings on the temperature dependence of protein secretion from the E. tarda T3SS and T6SS, suggesting that the PhoP-PhoQ system of E. tarda is only activated at the optimum growth temperature of the host to ensure the highest level of virulence and the survival of the bacteria. These findings also agree with the observation that outbreaks of acute E. tarda infection are mostly found in channel catfish culture systems when the temperature rises due to overcrowding (44). On the other hand, Mg 2ϩ concentration detection by the PhoP-PhoQ system seems to provide E. tarda with cues that it located inside the host body.…”

Section: Discussionsupporting

confidence: 90%

Temperature and Mg2+ Sensing by a Novel PhoP-PhoQ Two-component System for Regulation of Virulence in Edwardsiella tarda

Chakraborty

Chatterjee

et al. 2010

Journal of Biological Chemistry

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The PhoP-PhoQ two-component system is commonly used by bacteria to sense environmental factors. Here we show that the PhoP-PhoQ system of Edwardsiella tarda detects changes in environmental temperature and Mg 2؉ concentration as well as regulates the type III and VI secretion systems through direct activation of esrB. Protein secretion is activated from 23 to 35°C or at low Mg 2؉ concentrations, but it is suppressed at or below 20°C, at or above 37°C, or at high Mg 2؉ concentrations. The effects of temperature and Mg 2؉ concentration are additive. The PhoQ sensor domain has a low T m of 37.9°C, and it detects temperatures through a conformational change of its secondary structure. Mutation of specific Pro or Thr residues increased the stability of the PhoQ sensor drastically, altering its temperature-sensing ability. The PhoQ sensor detects Mg 2؉ concentration through the direct binding of Mg 2؉ to a cluster of acidic residues (DDDSAD) and through changes that likely affect its tertiary structure. Here, we describe for the first time the use of PhoP-PhoQ as a temperature sensor for bacterial virulence control.

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Section: Discussionsupporting

confidence: 90%

Temperature and Mg2+ Sensing by a Novel PhoP-PhoQ Two-component System for Regulation of Virulence in Edwardsiella tarda

Chakraborty

Chatterjee

et al. 2010

Journal of Biological Chemistry

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“…As the findings of Alcorn et al (2002) indicate that there are actually quite marked temperature effects on rainbow trout immune functions, this also supports the explanation made in this study on virulence category. Warming temperatures often increase the abundance and virulence of pathogenic bacteria such as Flavobacterium columnare (Skrodenyté-Arbačiauskiené et al, 2006), Edwardsiella tarda (Mohanty and Sahoo, 2007), Aeromonas salmonicida, A. hydrophila and Vibrio anguillarum (McCullough, 1999). In order to prevent and control A. cryaerophilus infections, the relationship between virulence of the pathogen and water temperature should be considered in further studies.…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

Gross pathology, blood chemistry, lipid and peroxide contents in rainbow trout ( Oncorhynchus mykiss Walbaum) affected by experimental Arcobacter cryaerophilus infection at low water temperature

Aydin

Gure²,

Çakici

et al. 2009

Acta Veterinaria Hungarica

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Arcobacter cryaerophilus was isolated from naturally infected rainbow trout ( Oncorhynchus mykiss Walbaum), and its pathogenicity was tested by intramuscular injection using healthy 1-year-old rainbow trout under cold-water conditions (at 5 °C). The lethal dosage of 50% end point (LD 50 ) for A. cryaerophilus was calculated as 7.79 × 10 5 viable cells. Experimental infection caused gross clinical abnormalities such as fallen scales, exophthalmia, oedema in injection region and at the base of fins, pale gills, kidney necrosis, hyperaemic areas in pale liver, haemorrhagic spots in heart, elongated spleen and swollen gallbladder. Activities of aspartate aminotransferase and alkaline phosphatase, and concentrations of glucose, total protein, albumin, cholesterol, triglyceride and calcium in the serum of the experimentally infected rainbow trout were significantly decreased compared with the healthy fish. Positive correlations were observed among blood parameters. Total lipid weights increased in the brain, muscle and liver tissues of infected fish and dropped in the gill and spleen tissues. Lipid peroxide contents in the brain, liver, kidney, spleen, muscle and gill tissues of infected rainbow trout were significantly higher than in healthy animals. The present work shows that A. cryaerophilus can be moderately virulent for rainbow trout at low water temperature, and changes in lipid and lipid peroxide contents of tissues and blood indices can highlight barely detectable effects of A. cryaerophilus infection in rainbow trout under laboratory conditions. However, the application of these indices in farm biomonitoring using rainbow trout will need more detailed studies and a careful consideration of the environmental parameters.

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“…Edwardsiellosis is the name of the disease caused by this pathogen, and is characterized by septicaemia, haemorrhages, internal abscesses and skin lesion in various fish taxa [2].…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence

Buján

Hernández-Haro

Monteoliva

et al. 2015

Journal of Proteomics

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Edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. This species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. In this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the Norwest of Spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. One hundred and three proteins that presented different abundance were successfully identified and classified into 11 functional categories according to their biological processes: amino acid, carbohydrate and lipid metabolism, tricarboxylic cycle, stress response and protein fate, protein synthesis, biogenesis of cellular components, cell rescue defence and virulence, cell membrane and transport, signal transduction and purine and pyrimidine metabolism. Twenty three protein spots detected only in turbot isolate were identified. It was shown that the same proteins appeared in different spots in the two isolates. Mass spectra obtained by MALDITOF/TOF of some of these proteins and DNA sequencing explained the changes as a result of different amino acid sequences. Several proteins related with the virulence of E. tarda (FliC, ArnA or FeSODI) were only detected in the turbot European isolate.

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Edwardsiellosis in fish: a brief review

Cited by 338 publications

References 103 publications

Temperature and Mg2+ Sensing by a Novel PhoP-PhoQ Two-component System for Regulation of Virulence in Edwardsiella tarda

Temperature and Mg2+ Sensing by a Novel PhoP-PhoQ Two-component System for Regulation of Virulence in Edwardsiella tarda

Gross pathology, blood chemistry, lipid and peroxide contents in rainbow trout ( Oncorhynchus mykiss Walbaum) affected by experimental Arcobacter cryaerophilus infection at low water temperature

Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence

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