This research aimed to determine the extent of the relevance of 2nd Biology laboratory practice subject matter with biology material. Laboratory practice is part of learning to test and apply a concept in the form of learning activities or in high school. The method used is descriptive. Data was obtained from Biology Education students who have taken 2nd Biology Laboratory practice courses towards students. Instrument used is questionnaire, and analyze data used is descriptive. The results obtained on the relevance of the experimental substance to improve the competence of practicing laboratory practice in schools are that 44.21% strongly agree, 42.41% agree, and 10.96% disagree. The usefulness of the substance of the experiment in improving the competence of carrying out biology laboratory practice in schools is that on average as much as 55.04% strongly agree, and as much as 43.84% agree. The conclusions and suggestions from this study need to include other alternative materials for the experiment, bearing in mind that not all laboratories in the schools have complete laboratory tools and materials.