EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells

Galler, Kerstin M.; Widbiller, Matthias; Buchalla, Wolfgang; Eidt, Andreas; Hiller, K-A; Hoffer, P C; Schmalz, Gottfried

doi:10.1111/iej.12492

Cited by 166 publications

(171 citation statements)

References 35 publications

(33 reference statements)

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“…2 In contrast, a smaller amount of TGF-b1 was found in the NaOCl treatment samples. Although our data broadly corroborate those of other studies showing that EDTA conditioning of dentine promotes migration of dental pulp stem cells, 3 we noticed differences in relation to published data regarding cell migration toward alpha-MEM supplemented with 20% FBS (Control 1). Whereas our study found that the rates of migration toward the media conditioned by tooth slices containing predentin layer and treated with 10% EDTA were similar to those of Control 1, 3 Galler et al (2016) found that cell migration toward the pretreated dentine surface amounted to 70% of the positive migration control (alpha-MEM with 20% FBS).…”

Section: Discussionsupporting

confidence: 83%

“…Several studies have focused on the regenerative potential of different cytokines and growth factors designed to improve cellular chemotaxis and cell homing into the emptied dental pulp space in vivo. 1,2,3,4,5,6 The dentin matrix is a reservoir of soluble cytokines and growth factors secreted by odontoblasts and pulp fibroblasts, which could be released during tissue demineralization. 1,2,3,4 The dissolution of these cytokines and growth factors can occur pathologically during dental caries and following restorative procedures.…”

Section: Introductionmentioning

confidence: 99%

“…1,2,3,4,5,6 The dentin matrix is a reservoir of soluble cytokines and growth factors secreted by odontoblasts and pulp fibroblasts, which could be released during tissue demineralization. 1,2,3,4 The dissolution of these cytokines and growth factors can occur pathologically during dental caries and following restorative procedures. 2,3 Notably, during severe pulp disease or trauma, primary odontoblasts may not survive, and formation of a Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.…”

Section: Introductionmentioning

confidence: 99%

“…17 EDTA condit ion i ng of dent i ne promotes adhesion, migration and differentiation of the pulp stem cells from permanent teeth. 3 In addition, various soluble proteins extracted from the dentine matrix are able to exert a direct biological effect on dental pulp stem cells in promoting mineralized tissue repair mechanisms. 19 Nevertheless, the amount of TGF-b1 released from dentin by different irrigants used in endodontic procedures (such as EDTA and NaOCl) has not been clearly established.…”

Section: Introductionmentioning

confidence: 99%

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Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

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Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

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“…8,12 Without these growth factors, the intrinsic signals embedded in dentin and liberated by root canal irrigants such as ethylenediaminetetraacetic acid (EDTA) can also produce an apparently functional pulp. 10,13 As a result, the application of the cell homing concept appears efficient to induce repair/ regenerative processes within the pulp space and can therefore circumvent some disadvantages of a natural blood clot.…”

Section: Introductionmentioning

confidence: 99%

Effects of Stem Cell Factor on Cell Homing During Functional Pulp Regeneration in Human Immature Teeth

Ruangsawasdi

Zehnder

Patcas

et al. 2017

Tissue Engineering Part A

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Conventional root canal treatment in immature permanent teeth can lead to early tooth loss in children because root formation is discontinued. We investigated whether the stem cell factor (SCF) could facilitate cell homing in the pulpless immature root canal and promote regeneration of a functional pulp. In vitro, human mesenchymal stem cells (hMSCs) were exposed to SCF at various concentrations for assessing cell migration, proliferation, and differentiation toward odonto/osteoblasts by 3D-chemotaxis slides, WST-1 assay, and alkaline phosphatase activity, respectively. Fibrin gels were used to deliver 15 g/mL SCF for in vivo experiments. The release kinetic of SCF was assessed in vitro. Two corresponding human immature premolars, with or without SCF, were placed at rat calvariae for 6 and 12 weeks. All tooth specimens were either analyzed histologically and the percentage of tissue ingrowth determined or the cells were extracted from the pulp space, and the mRNA level of DMP1, DSPP, Col1, NGF, and VEGF were assessed by quantitative polymerase chain reaction. In the presence of SCF, we saw an increase in hMSCs directional migration, proliferation, and odonto/osteogenic differentiation. SCF also increased the extent of tissue ingrowth at 6 weeks but not at 12 weeks. However, at this time point, the formed tissue appeared more mature in samples with SCF. In terms of gene transcription, DMP1, Col1, and VEGF were the significantly upregulated genes, while DSPP and NGF were not affected. Our results suggest that SCF can accelerate cell homing and the maturation of the pulp-dentin complex in human immature teeth. Conventional root canal treatment in immature permanent teeth can lead to early tooth loss in children because root formation is discontinued. We investigated whether the stem cell factor (SCF) could facilitate cell homing in the pulpless immature root canal and promote regeneration of a functional pulp. In vitro, human mesenchymal stem cells (hMSCs) were exposed to SCF at various concentrations for assessing cell migration, proliferation, and differentiation toward odonto/osteoblasts by 3D-chemotaxis slides, WST-1 assay, and alkaline phosphatase activity, respectively. Fibrin gels were used to deliver 15 mg/mL SCF for in vivo experiments. The release kinetic of SCF was assessed in vitro. Two corresponding human immature premolars, with or without SCF, were placed at rat calvariae for 6 and 12 weeks. All tooth specimens were either analyzed histologically and the percentage of tissue ingrowth determined or the cells were extracted from the pulp space, and the mRNA level of DMP1, DSPP, Col1, NGF, and VEGF were assessed by quantitative polymerase chain reaction. In the presence of SCF, we saw an increase in hMSCs directional migration, proliferation, and odonto/ osteogenic differentiation. SCF also increased the extent of tissue ingrowth at 6 weeks but not at 12 weeks. However, at this time point, the formed tissue appeared more mature in samples with SCF. In terms of gene transcription, DMP1, Col1, a...

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Treatment of Immature Teeth with Pulp Necrosis

Cooper

Duncan

Widbiller

et al. 2021

Endodontic Materials in Clinical Practice

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EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells

Cited by 166 publications

References 35 publications

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

Effects of Stem Cell Factor on Cell Homing During Functional Pulp Regeneration in Human Immature Teeth

Treatment of Immature Teeth with Pulp Necrosis

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