Editorial for special issue on green biomanufacturing

Lv, Yongqin; Su, Haijia; Tan, Tianwei

doi:10.1016/j.synbio.2020.10.006

Cited by 7 publications

(12 citation statements)

References 11 publications

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“…Green biomanufacturing is a significant landmark for upgradation of traditional chemical industry as it employs industrial biotechnology for cleaner production of biochemicals, biofuels, and bioproducts with enhanced energy by sustainable biosystems (Lv et al, 2020). In green biomanufacturing system, industrial biotechnology, and chemical engineering technology contribute to yield useful products of interest by utilizing biomass and other renewable raw materials.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing

Zhang

Guo

et al. 2022

Biotech & Bioengineering

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Green biological manufacturing is a revolutionary industrial model utilizing yeast as a significant microbial cell factory to produce biofuels and other biochemicals. However, biotransformation efficiency is often limited owing to several stress factors resulting from environmental changes or metabolic imbalance, leading to the slow growth of cells, compromised yield, and enhanced energy consumption. These factors make biological manufacturing competitively less economical. In this regard, minimizing the stress impact on microbial cell factories and strong robust performance have been an interesting area of interest in the last few decades. In this review, we focused on revealing the stress factors and their associated mechanisms for yeast in biological manufacturing. To improve yeast tolerance, rational and irrational strategies were introduced, and the molecular basis of genome evolution in yeast was also summarized. Furthermore, strategies of genome‐directed evolution such as homology directed repair and nonhomologous end‐joining, and the synthetic chromosome recombination and modification by LoxP‐mediated evolution and their association with stress tolerance was highlighted. We hope that genome evolution provides new insights for solving the limitations of the natural phenotypes of microorganisms in industrial fermentation for the production of valuable compounds.

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Section: Introductionmentioning

confidence: 99%

Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing

Zhang

Guo

et al. 2022

Biotech & Bioengineering

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“…As a new, low-cost, and high-capacity industrial production model, green bio-manufacturing has emerged as a leading force in the manufacturing industry due to its advantages in sustainable development. 2 With continuous technological innovation and progress, the product space of green bio-manufacturing has been further expanded, and the development prospects are very promising. 3 However, there are also challenges to address.…”

Section: Introductionmentioning

confidence: 99%

Improving thermo-tolerance of Saccharomyces cerevisiae by precise regulation of the expression of small HSP

Zhang,

et al. 2023

RSC Adv.

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“…There needs to be a global transition from an overreliance on petrochemical feedstocks, polluting chemical processes and non-sustainable practices towards green biomanufacturing using microorganisms to access renewable carbon sources ( Lv et al, 2020 ). A surge in the development of green biomanufacturing technologies over the past decade, in part realized through the engineering of biology, has encompassed diverse applications within the biopharmaceutical, energy, chemicals, and advanced synthetic fuels sectors.…”

Section: Introductionmentioning

confidence: 99%

“…A surge in the development of green biomanufacturing technologies over the past decade, in part realized through the engineering of biology, has encompassed diverse applications within the biopharmaceutical, energy, chemicals, and advanced synthetic fuels sectors. This interest has led to the use of robust industrial microbial hosts, de novo pathway constructions for new and existing chemicals production and utilization of renewable and sustainable carbon sources as feedstocks ( Lv et al, 2020 ). Biomanufacturing routes have the potential to make virtually any chemical from biogenic waste feedstocks.…”

Section: Introductionmentioning

confidence: 99%

Bioproduction of Linalool From Paper Mill Waste

Rinaldi

Tait

Toogood

et al. 2022

Front. Bioeng. Biotechnol.

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A key challenge in chemicals biomanufacturing is the maintenance of stable, highly productive microbial strains to enable cost-effective fermentation at scale. A “cookie-cutter” approach to microbial engineering is often used to optimize host stability and productivity. This can involve identifying potential limitations in strain characteristics followed by attempts to systematically optimize production strains by targeted engineering. Such targeted approaches however do not always lead to the desired traits. Here, we demonstrate both ‘hit and miss’ outcomes of targeted approaches in attempts to generate a stable Escherichia coli strain for the bioproduction of the monoterpenoid linalool, a fragrance molecule of industrial interest. First, we stabilized linalool production strains by eliminating repetitive sequences responsible for excision of pathway components in plasmid constructs that encode the pathway for linalool production. These optimized pathway constructs were then integrated within the genome of E. coli in three parts to eliminate a need for antibiotics to maintain linalool production. Additional strategies were also employed including: reduction in cytotoxicity of linalool by adaptive laboratory evolution and modification or homologous gene replacement of key bottleneck enzymes GPPS/LinS. Our study highlights that a major factor influencing linalool titres in E. coli is the stability of the genetic construct against excision or similar recombination events. Other factors, such as decreasing linalool cytotoxicity and changing pathway genes, did not lead to improvements in the stability or titres obtained. With the objective of reducing fermentation costs at scale, the use of minimal base medium containing paper mill wastewater secondary paper fiber as sole carbon source was also investigated. This involved simultaneous saccharification and fermentation using either supplemental cellulase blends or by co-expressing secretable cellulases in E. coli containing the stabilized linalool production pathway. Combined, this study has demonstrated a stable method for linalool production using an abundant and low-cost feedstock and improved production strains, providing an important proof-of-concept for chemicals production from paper mill waste streams. For scaled production, optimization will be required, using more holistic approaches that involve further rounds of microbial engineering and fermentation process development.

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Editorial for special issue on green biomanufacturing

Cited by 7 publications

References 11 publications

Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing

Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing

Improving thermo-tolerance of Saccharomyces cerevisiae by precise regulation of the expression of small HSP

Bioproduction of Linalool From Paper Mill Waste

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